Method for simultaneously separating soy protein and oil fat with inverse micelle abstraction technique
A technology of simultaneous separation and extraction technology, applied in the field of high-efficiency separation technology of new soybean protein and oil, can solve the problems of unstable product quality, large consumption of acid and alkali, difficult control, etc., to reduce production costs and investment in fixed assets, Simplify the production process and solve the effect of polluting the environment
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Embodiment 1
[0037] Add 80g of pretreated full-fat soybean powder into 4L80g / L of AOT reverse micelles solution, control the conditions, the buffer in the reverse micelles solution is NaNO 3 - Phosphate buffer, control condition W 0 9. NaNO 3 The concentration is 0.05mol / L, the pH is adjusted to 8.0, the extraction temperature is 45°C, and mechanically stirred at a speed of 180r / min for 2h. The pre-extraction rate of protein is 60.0%.
[0038] Add an equal volume of 1.0mol / L NaCl-phosphate buffer solution to adjust the pH value to 7.7, shake at a speed of 300r / min for 1.5h, centrifuge for 0.5h, and statically separate to obtain the upper layer of mixed oil and the lower layer of protein solution. The post-extraction rate of protein is 87.1%. The oil extraction rate is 90.0%.
[0039] Overall, the protein extraction rate was 52.3%, and the oil extraction rate was 90.0%. The foamability, emulsification and emulsification stability of soybean protein are better than those produced by alk...
Embodiment 2
[0041] 0.3g of pretreated full-fat soybean flour was added to 20mL of reverse micellar solution with AOT concentration of 120g / L, the buffer in the reverse micellar solution was KCl-phosphate buffer, and the control condition was W 0 18. KCl concentration 0.05mol / L, adjust pH 7.5, extraction temperature 40°C, shake at 180r / min for 1h. The pre-extraction rate of protein is 64.6%.
[0042] Add an equal volume of 1.0mol / L KCl-phosphate buffer solution to adjust the pH value to 7.0, shake in the shaker at a speed of 250r / min or more for 2 hours, centrifuge for 15 minutes, and statically separate to obtain the upper layer of mixed oil and the lower layer of protein solution. The post-extraction rate of protein is 89.0%. The oil extraction rate is 86.0%. Overall, the protein extraction rate was 57.5%, and the oil extraction rate was 86.0%.
Embodiment 3
[0044] 15g of pretreated full-fat soybean flour was added to 1L of reverse micelles solution with AOT concentration of 80g / L, and 2.0% Alcalase alkaline protease was added at the same time, and the control condition was W 0 20, KCl concentration 0.10mol / L, adjust pH 7.0, extraction temperature 45°C, extraction 2h. The pre-extraction rate of protein was 73.8%.
[0045] Add an equal volume of 0.8mol / L KCl-phosphate buffer to adjust the pH value to 8.0, shake in the shaker at a speed of 250r / min or more for 1.5h, centrifuge for 20min, and statically separate to obtain the upper layer of mixed oil and the lower layer of protein solution. The post-extraction rate of protein is 82.8%. The oil extraction rate is 87.5%.
[0046] Overall, the protein extraction rate was 61.1%, and the oil extraction rate was 86.0%. Adding Alcalase alkaline protease for extraction, the degree of proteolysis is about 4%, and the extraction rate is improved.
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