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Continuous culture process of anaerobe strain

A cultivation method and technology of anaerobic bacteria, applied in the field of continuous cultivation of caproic acid bacteria, can solve the problems of microbial domestication, slow growth rate, and low number of beneficial microorganisms, and achieve the effects of simple operation, enhanced anti-bacteria ability, and continuous cultivation

Inactive Publication Date: 2008-11-26
周剑平
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, although the cultivation technology of artificial old pit mud has become more and more mature, overall, the artificially cultivated fermentation tanks cannot achieve high-quality and high-yield effects in a short period of time.
The main reasons are microbial domestication, slow growth rate, and low number of beneficial microorganisms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The carrier of immobilized cells—polyvinyl alcohol (PVA) composite gel is synthesized as follows: slowly dissolve 9 grams of polyvinyl alcohol into 100 ml of geothermal water at 85°C to 95°C, stir while adding, and wait until the polyethylene After the alcohol is completely dissolved, add 4 ml of glycerol, stir evenly, then add 1 gram of borax that has been dissolved (dissolved with 1:1 hot water) while stirring continuously, and stir while adding to form a gel. Chemical treatment, the crosslinking time is 2 to 15 minutes, then slowly add 1 gram of maleic anhydride (dissolved with 1:1 hot water), and keep stirring until there is no floc coagulation in the gel solution, The obtained transparent, slightly milky white viscous sol is polyvinyl alcohol composite gel.

[0013] In the anaerobic box, add 10mL of caproic acid bacteria suspension to 90mL of the above polyvinyl alcohol composite gel solution, stir evenly, pour it into a petri dish and spread it into a flat plate, ...

Embodiment 2

[0015] Repeat embodiment 1, have following difference: in anaerobic box, 5mL caproic acid bacteria suspension is added in the composite gel solution, make immobilized caproic acid bacteria, then cultivate in sodium acetate medium, temperature is 37°C. The number of caproic acid bacteria in the proliferation solution reached 0.8×10 9 per mL, the acidity reached 0.1g / 100mL.

Embodiment 3

[0017] Repeat embodiment 1, have following difference: in anaerobic box, 15mL caproic acid bacteria suspension is added in the above-mentioned composite gel solution, make immobilized caproic acid bacteria, then cultivate in sodium acetate medium, The temperature was 37°C. The number of caproic acid bacteria in the proliferation solution reached 1.2×10 9 per mL, the acidity reached 0.46g / 100mL.

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Abstract

The invention discloses a continuous culture method for anaerobic strains which take polyvinyl alcohol complex gel as embedding material. The method comprises the following steps: a) mixing: 5 to 15mL caproic acid bacteria suspension fluid is added into 90mL polyvinyl alcohol complex gel solution in an anaerobic box; after being stirred evenly, the mixture is poured into a culture dish and paved into a plate; b) freezing and cutting into blocks: the mixture is frozen for 48 hours at the temperature of minus 20 DEG C, after taking shape, the mixture is taken out and cut into blocks with a boundary dimension of 12mm*10mm*12mm; c) culture: then the mixture is cultured in sodium acetate medium at the temperature of 37 DEG C. The method can realize continuous culture with simple operation and significant function of increasing the caproic acid bacteria and decreasing emulsion, so the method is suitable for extensive large industrialized production.

Description

technical field [0001] The invention relates to a method for continuously culturing anaerobic bacteria strains, in particular to a method for continuously cultivating caproic acid bacteria using polyvinyl alcohol composite gel as an embedding material. Background technique [0002] Anaerobic bacteria cannot grow in the presence of oxygen. To cultivate anaerobic bacteria, an oxygen-free environment must be created. Usually, reducing agents are added to the medium, or free oxygen in the environment is removed by physical and chemical methods to reduce the redox potential. Such as sodium thioglycolate medium, bovine heart and brain infusion medium, etc. Commonly used anaerobic culture methods include anaerobic cylinder method, anaerobic bag, anaerobic box, biological oxygen consumption method, etc., which can be selected according to the actual situation. [0003] In recent years, although the cultivation technology of artificial old pit mud has become more and more mature, g...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 周剑平王治业杨晖魏甲乾
Owner 周剑平
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