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Method for agro-bacteriumrhizogenes transferred plant rooting to cultivating plant parasite wireworm

A technique for transforming plants with Agrobacterium rhizogenes, which is applied in the field of cultivating plant parasitic nematodes by using Agrobacterium rhizogenes to transform plant hairy roots. The effect of convenient root subculture, avoiding the decline of nematode vigor, and convenient separation

Inactive Publication Date: 2006-02-22
INST OF PLASMA PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

[0007] Since the vast majority of plant parasitic nematodes cannot be cultured without exogenous sources, a large number of nematodes can be isolated from the host plant culture as research objects. The disadvantage is that due to the complexity of the relationship between plant parasitic nematodes and plant hosts, it is difficult to study their biochemistry; callus Single exogenous culture on tissue or isolated root is a commonly used method to study the biochemical and genetic development characteristics of plant parasitic nematodes. The disadvantage is that the number of cultured nematodes is small and cannot be subcultured, which affects the continuity of the experiment.

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  • Method for agro-bacteriumrhizogenes transferred plant rooting to cultivating plant parasite wireworm

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Embodiment Construction

[0045] Because Agrobacterium rhizogenes can infect most dicotyledonous plants, a few monocotyledonous plants and individual gymnosperms, and induce the injured parts of infected plants to grow hair roots, it is possible to establish important crops that Agrobacterium rhizogenes can infect , economic crops and fruit trees, etc. root system. And applied to the cultivation and identification of plant parasitic nematodes. Cultivating the sweet potato stem nematode below with carrot hairy root is embodiment, and its process is as follows:

[0046]1. Induction of carrot hair roots

[0047] Wash the carrots, peel off the outer skin of the carrots, disinfect the surface with 0.05% sodium hypochlorite for 20 minutes, rinse with sterile distilled water 5 times, and then cut into 0.5 - 1cm thick, inoculate carrot slices in 1% water agar medium (the bottom of carrot slices is immersed in 1% water agar medium, and the upper surface is exposed), and take a drop of activated Agrobacterium ...

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Abstract

The invention discloses a method for culturing plant-parasitic nematodes through conversing plant hairy roots with agrolbacterium, which comprises, under asepsis conditions, promoting rapid roots growth of agrolbacterium conversion plants by utilizing culture-medium containing no hormone, providing sufficient nutriment for the growth, development and breeding of plant parasitic nematodes.

Description

technical field [0001] The invention belongs to a method for cultivating plant parasitic nematodes, in particular to a method for cultivating plant parasitic nematodes by transforming plant hairy roots with Agrobacterium rhizogenes. Background technique [0002] Plant parasitic nematodes are an important class of pathogenic organisms, many of which are internationally recognized as devastating pests such as pine wood nematode (Bursaphelenchus xylophilus), banana nematode (Radopholussimilis), potato white nematode (G.loboderapallida) and potato gold nematode (G.rostochiensis), etc., the annual economic losses caused by plant parasitic nematodes in the world exceed 100 billion US dollars. The harm of plant parasitic nematodes to plants is not only manifested in the mechanical damage caused by plundering host plant nutrition and feeding activities, but more importantly, its esophageal gland secretions cause a series of pathological changes in host plants and spread other pathog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12N5/06A01H4/00
Inventor 阮龙肖翔王钰刘兵吴跃进许安余增亮
Owner INST OF PLASMA PHYSICS CHINESE ACAD OF SCI
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