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High-temperature acid-resistant alpha-amylase mutant strain and construction method thereof

A construction method and amylase technology, applied in the field of bioengineering, can solve problems such as loss of enzyme activity and inability to adapt, and achieve the effects of stable enzyme activity, saving industrial grain, good thermal stability and acid stability

Active Publication Date: 2011-05-04
TIANJIN UNIV OF SCI & TECH
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Problems solved by technology

[0007] It can be seen that the optimum temperature of acid α-amylase in China is lower than 70°C at present. Because the enzyme activity will be greatly lost when the hydrolysis reaction exceeds 70°C, it cannot adapt to the starch deep processing industries such as alcohol and starch saccharification in my country. Require

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  • High-temperature acid-resistant alpha-amylase mutant strain and construction method thereof
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  • High-temperature acid-resistant alpha-amylase mutant strain and construction method thereof

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[0030] The following examples are used to further illustrate the present invention. The following examples are illustrative and not restrictive. The following examples cannot be used to limit the protection scope of the present invention.

[0031] The present invention combines the obtained high-temperature acid-resistant alpha-amylase (acid-resistant modification of alpha-amylase and its secretion expression in Bacillus subtilis. Food and Fermentation Industry, 2005, 31(10): 33-36. High-temperature alpha-amylase and its preparation method, patent application number: 200510013865.2), in order to improve the high-temperature resistant alpha-starch isolated from Bacillus licheniformis (China Industrial Microbial Culture Collection and Management Center, CICC, preservation number, 10181) The acid resistance of the enzyme is a mutant gene obtained by changing L-leucine at position 134 of the mature peptide of this gene to L-arginine and changing L-serine at position 320 to L-alanine; ...

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Abstract

The invention relates to a mutant strain of a high temperature acid-proof Alpha-amylase and a method for producing the mutant strain. The high temperature acid-proof Alpha-amylase gene is obtained by mutating the Bacillus licheniformis high-temperature resistant Alpha-amylase gene (Leu134 Arg and Ser320 Ala); the high temperature acid-proof Alpha-amylase gene is integrated into the genom DNA of the parent strain Bacillus licheniformis by establishing a target carrier and by adopting the method of homologous recombination so that the Bacillus licheniformis mutate strain is obtained to realize the homology effective expression of the high temperature acid-proof Alpha-amylase gene. The Alpha-amylase produced by the mutate strain is resistant to high temperature and has the acid stability improved evidently, thereby providing good technique condition for the starch deep processing industry such as alcohol and mashing in China, lowering the cost, saving the energy and the industrial grain,and satisfying the requirements of the starch raw material liquefying technique on acid conditions.

Description

Technical field [0001] The invention relates to the field of bioengineering, in particular to a mutant strain of high-temperature and acid-resistant alpha-amylase and a construction method thereof. Background technique [0002] α-Amylase (α-1,4-glucan-glucanhydrolasa EC 3.2.1.1) is also known as liquefaction type amylase, which can arbitrarily cut α-1,4 bonds from the inside of starch molecules to produce maltose with a relatively small molecular weight dextrin. Under the action of α-amylase, starch molecules are rapidly degraded, and the viscosity decreases, and liquefaction is completed. Alpha-amylase has considerable commercial value and is widely used in starch deep processing industry, alcohol industry, beer industry, citric acid industry, monosodium glutamate and starch saccharification industry, pharmaceutical industry and textile industry. Alpha-amylase can be produced by fermentation of microorganisms, and can also be extracted from plants and animals. At present, mos...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C12N15/56C12N15/75C12N15/90C12R1/10
Inventor 路福平杜连祥刘逸寒李玉王建玲王春霞
Owner TIANJIN UNIV OF SCI & TECH
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