Preparation and application of synthetic polypeptide medicaments for treating tubercle bacillus infection contamination
A technology of bacillus and antimicrobial peptides, applied in the field of biomedicine, can solve the problem that there are no relevant literature reports on the pharmacological active ingredients of Tianlong's anti-tuberculosis, and achieve the effect of growth inhibition
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Embodiment 1
[0018] Example 1: The preparation method of Tianlong antimicrobial peptide
[0019] Crush the dried medicinal materials of Tianlong through an 80-mesh sieve, weigh 2500g of the medicinal powder, mix it with 4500ml of distilled water to swell, leave it in a 4-degree refrigerator for 24 hours, centrifuge at low temperature and high speed to obtain 800ml of supernatant, and filter it with a 0.45um membrane to obtain the crude extraction of Tianlong antimicrobial peptide The crude extract was passed through a solid phase extraction column ODS-SPE (C18, capped), eluted with water and methanol respectively, and freeze-dried to obtain a water-soluble part and an alcohol-soluble part, which were analyzed by HPLC chromatography (ODS C18 column, SHIMADZU 150L* 4.6), the methanol-soluble part was separated and purified by gel filtration column chromatography, dialysis desalination and reversed-phase high-pressure liquid chromatography to obtain the polypeptide.
[0020] in:
[0021] Gel...
Embodiment 2
[0026] Example 2 Antimicrobial Peptides Research on the Bacteriostasis of Mycobacteria
[0027] 1. Medium
[0028] 7H9 agar semi-flow medium; Middlebrook 7H9 dry powder (SIGMA M0178) 4.7g, glycerol 4ml, agar powder 0.5g, add distilled water to 900ml, 15 pounds 121 ℃ autoclave for 10 minutes, add OADC to 1000ml after cooling, wait use.
[0029] Sutong semi-fluid medium: weigh 4.0g sodium glutamate, 1g citric acid, K 2 HPO 4 0.25g, MgSO 4 ·7H 2 O 0.25g, ferric ammonium citrate 0.025g, glycerol 15ml, agar powder 0.25g, add distilled water and heat to make it dissolve completely, adjust pH to 7.2 with 8% NaOH after cooling, dilute to 500ml with distilled water, pack, Autoclave at 121°C for 20 minutes and set aside.
[0030] 2. Drugs: The control drug is isoniazid; the test drug is Denon antimicrobial peptide.
[0031] 3. Strains: Standard strain of Mycobacterium tuberculosis (Mycobacterium tuberculosis H 37 Rv, ATCC 27294); Mycobacterium aureus (M.aurum, ATCC 23366); Isoni...
Embodiment 3
[0041] Female, 41 years old, was diagnosed with pulmonary tuberculosis in 2000 due to cough and sputum. After treatment with isoniazid, resifopin, pyrazinamide and ethambutol, the condition did not improve significantly, and the drug sensitivity test was polyresistant Drug-refractory tuberculosis (resistant to isoniazid and resifopin), cough, sputum, positive sputum bacteria, and empty lungs. The antibacterial peptide 20 mg was taken orally, tid, and maintained for 3 weeks, the cavity was closed, the sputum bacteria turned negative, and the clinical symptoms disappeared, so the drug was stopped.
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