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Preparation and application of synthetic polypeptide medicaments for treating tubercle bacillus infection contamination

A technology of bacillus and antimicrobial peptides, applied in the field of biomedicine, can solve the problem that there are no relevant literature reports on the pharmacological active ingredients of Tianlong's anti-tuberculosis, and achieve the effect of growth inhibition

Inactive Publication Date: 2008-10-29
王鹤尧 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Yu Lijun et al.’s patent application “Traditional Chinese Medicine for Treating Tuberculosis and Its Preparation Method” (Patent No. 03132605.6) reported that Tianlong is used to treat scrofula and tuberculosis. Although Tianlong is effective in treating tuberculosis, there is no information about the pharmacological active ingredients of Tianlong against tuberculosis Related literature reports

Method used

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  • Preparation and application of synthetic polypeptide medicaments for treating tubercle bacillus infection contamination
  • Preparation and application of synthetic polypeptide medicaments for treating tubercle bacillus infection contamination

Examples

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Effect test

Embodiment 1

[0018] Example 1: The preparation method of Tianlong antimicrobial peptide

[0019] Crush the dried medicinal materials of Tianlong through an 80-mesh sieve, weigh 2500g of the medicinal powder, mix it with 4500ml of distilled water to swell, leave it in a 4-degree refrigerator for 24 hours, centrifuge at low temperature and high speed to obtain 800ml of supernatant, and filter it with a 0.45um membrane to obtain the crude extraction of Tianlong antimicrobial peptide The crude extract was passed through a solid phase extraction column ODS-SPE (C18, capped), eluted with water and methanol respectively, and freeze-dried to obtain a water-soluble part and an alcohol-soluble part, which were analyzed by HPLC chromatography (ODS C18 column, SHIMADZU 150L* 4.6), the methanol-soluble part was separated and purified by gel filtration column chromatography, dialysis desalination and reversed-phase high-pressure liquid chromatography to obtain the polypeptide.

[0020] in:

[0021] Gel...

Embodiment 2

[0026] Example 2 Antimicrobial Peptides Research on the Bacteriostasis of Mycobacteria

[0027] 1. Medium

[0028] 7H9 agar semi-flow medium; Middlebrook 7H9 dry powder (SIGMA M0178) 4.7g, glycerol 4ml, agar powder 0.5g, add distilled water to 900ml, 15 pounds 121 ℃ autoclave for 10 minutes, add OADC to 1000ml after cooling, wait use.

[0029] Sutong semi-fluid medium: weigh 4.0g sodium glutamate, 1g citric acid, K 2 HPO 4 0.25g, MgSO 4 ·7H 2 O 0.25g, ferric ammonium citrate 0.025g, glycerol 15ml, agar powder 0.25g, add distilled water and heat to make it dissolve completely, adjust pH to 7.2 with 8% NaOH after cooling, dilute to 500ml with distilled water, pack, Autoclave at 121°C for 20 minutes and set aside.

[0030] 2. Drugs: The control drug is isoniazid; the test drug is Denon antimicrobial peptide.

[0031] 3. Strains: Standard strain of Mycobacterium tuberculosis (Mycobacterium tuberculosis H 37 Rv, ATCC 27294); Mycobacterium aureus (M.aurum, ATCC 23366); Isoni...

Embodiment 3

[0041] Female, 41 years old, was diagnosed with pulmonary tuberculosis in 2000 due to cough and sputum. After treatment with isoniazid, resifopin, pyrazinamide and ethambutol, the condition did not improve significantly, and the drug sensitivity test was polyresistant Drug-refractory tuberculosis (resistant to isoniazid and resifopin), cough, sputum, positive sputum bacteria, and empty lungs. The antibacterial peptide 20 mg was taken orally, tid, and maintained for 3 weeks, the cavity was closed, the sputum bacteria turned negative, and the clinical symptoms disappeared, so the drug was stopped.

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Abstract

The invention provides a preparation method of a derivative of an antimicrobial peptide derived from Gekko sp. for treating Mycobacterium tuberculosis infection, belonging to the field of biomedicine. The antimicrobial peptide derivative is a single chain polypeptide synthesized from a natural polypeptide isolated from Chinese amphibian Gekko sp. The primary complete sequence of the polypeptide is: Val-Ser-Asn-Ala-Ala-Thr-His-Leu-Cys-Lys-His-Ala-His-Cysteine-His-Phe-Arg-Asp-Val-Cys-His-Asn-Trp-Met-Arg-His-Phe-Thr-Cys-Arg (NH2-VSNAATH LCKH AHC HF RDVCHNW MRHFTCR-COOH). The inventive antimicrobial peptide derivative can remarkably inhibit the growth of Mycobacterium tuberculosis including multi-drug resistant strains, and can be used in preparing drugs for treating infection of Mycobacterium tuberculosis including multi-drug resistant strains.

Description

Technical field: [0001] The invention provides a Tianlong antimicrobial peptide medicine and a preparation method thereof, which are used for treating multidrug-resistant Mycobacterium tuberculosis infection, and belong to the technical field of biomedicine. technical background: [0002] (1) Current status of multi-drug resistant tuberculosis [0003] Tuberculosis is one of the infectious diseases that threaten human health the most. According to WHO statistics, currently about 2 billion people in the world are infected with Mycobacterium tuberculosis, with about 8 million new cases each year and about 3 million deaths, accounting for 7% of the population mortality rate. One of the main reasons for the increase in the incidence and mortality of tuberculosis is the emergence and spread of drug-resistant strains of conjugating bacilli. According to reports, about 36% of global tuberculosis patients are infected with drug-resistant bacteria, and this phenomenon is particular...

Claims

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Application Information

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IPC IPC(8): C07K14/46A61K38/17A61P31/04
Inventor 郑文婕王鹤尧孔繁翠顾昊邱葵
Owner 王鹤尧
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