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Mixed endophyte and method for preparing huperzine A by using the endophyte

A technology of huperzine A and endophyte, which is applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of difficulty in artificial synthesis, high price, and low content of whole grass.

Inactive Publication Date: 2008-10-01
洪亚辉
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AI Technical Summary

Problems solved by technology

[0004] However, due to the complex structure of huperzine A, artificial synthesis is very difficult and the content in the whole plant is very small, and the content in Melaleuca is only about one ten thousandth, and the plant source of Melaleuca is difficult and the growth cycle is as long as 8 to 10 years , has not yet been cultivated artificially. At present, the production of medicines is completely dependent on wild resources, and the price is expensive.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Endophyte Isolation

[0017] Take the skinned stem of Melaleuca pagoda, disinfect it with 75% alcohol for 5 minutes, then disinfect it with 0.1% mercury liter for 10 minutes, rinse it several times with sterile water, and inoculate.

[0018] Inoculate the sterilized material onto MS medium. (1000ml: agar 7g, sucrose 30g, inorganic large amount 100ml, inorganic trace 10ml, organic matter 10ml, iron salt 10ml, 1mg / lKT, 0.1mg / LNAA, PH5.8) 26 ℃ constant temperature culture for about 10 days, pick different forms The spores of the bacterial colony are transferred to PDA medium (1000ml: 200g of peeled potatoes, 20g of glucose, 15-20g of agar) on the PDA medium for constant temperature cultivation at 26°C. Endophytes were induced by culture, and spores of colonies with different shapes were picked and cultured on PDA medium to obtain endophytes.

[0019] Strain fermentation culture

[0020] Pick a small amount of mycelium and carry out fermentation culture respectively.

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Abstract

The present invention provides a mixing endophyte, especially an endophyte separated from plants. The invention also relates to the use of the endophyte. The mixing endophyte of the invention is separated from the plant huperzia serrata. The invention also provides a method of huperzine a prepared by the obtained endophyte. The invention provides a separating and culturing endophyte from the huperzia serrata plant for the first time, a huperzine a compound is extracted from the broth and the mycelium when the mixing endophyte is cultured and fermented. The invention provides a simple path for the compound preparation, having a low cost, the rare plant is not damaged.

Description

technical field [0001] The present invention relates to a mixed endophyte, especially endophyte isolated from plants. [0002] The present invention also relates to the use of the endophyte, that is, the method for preparing huperzine A by using the endophyte. Background technique [0003] Huperzine A (Hup A for short), a piperidine derivative, also known as fordine, is a natural alkaloid isolated from Melaleuca tower, which is a potent reversible acetylcholinesterase (ACHE ) inhibitor, which has a good effect on improving learning and memory and memory function of the elderly, and has a significant effect on the treatment of myasthenia gravis and senile dementia. Clinically, huperzine A (drug name Haberin) has been used to treat memory loss and early senile dementia in the elderly for various reasons and in different age groups. It is currently the most effective drug for the above diseases at home and abroad. Due to its high therapeutic index and long duration of action,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
Inventor 洪亚辉刘仲华肖浪涛
Owner 洪亚辉
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