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Method for latency fingerprint appearance of surface functionalization nano-gold particle

A gold nanoparticle and gold nanoparticle technology, which is applied in the surface functionalized gold nanoparticle and potential fingerprint display fields, can solve the problems of DNA test sensitivity and result interference, health hazards of professional and technical personnel, and no breakthrough progress, etc. , to achieve the effects of no toxic side effects, clear contrast, and safe use

Inactive Publication Date: 2008-09-24
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common weakness of these methods is low sensitivity, some of which interfere with the sensitivity and results of DNA testing
There are still hidden dangers in the existing visualization reagents and visualization methods, such as the small particle size dust suspended in the air when the brush visualization method is adopted, some carcinogenic fluorescent dyes, and toxic and harmful gases generated by the decomposition of substances during the fumigation method. cause serious harm to the health of professional and technical personnel
The most widely used powder method at present, the fatal weakness of this method is that it cannot reveal old fingerprints
Due to the limitation of safety and cost, the development of new gasification agent has not made breakthrough progress
The background fluorescence produced by the photoluminescence technology currently used at the same time has a great interference with the conventional photoluminescence bioprinting recognition, and it is difficult to meet the expected requirements.

Method used

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  • Method for latency fingerprint appearance of surface functionalization nano-gold particle
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  • Method for latency fingerprint appearance of surface functionalization nano-gold particle

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Preparation of thiol-modified gold nanoparticles, i.e. probe 1, and fingerprint development method-(1) Preparation of dodecylthiol-modified gold nanoparticles:

[0034] (1) at room temperature, the chloroauric acid aqueous solution of 30mmol / L and the toluene solution of the tetraoctylammonium bromide of 50mmol / L are mixed and stirred, until chloroauric acid is completely transferred in the organic phase toluene;

[0035] (2) adding dodecyl mercaptan in the organic phase;

[0036] (3) With vigorous stirring, the newly prepared 0.4mol / L sodium borohydride ice solution was slowly added to the mixed solution, and the organic phase was separated after continuing to stir for 3 hours;

[0037] (4) The remaining solution was rotary evaporated at 40°C, then ethanol was added and kept at -18°C for 4 hours, and the obtained purple-black precipitate was the hydrophobized dodecylthiol-protected gold nanoparticles.

[0038]Fingerprint development process: At room temperature, probe...

Embodiment 2

[0041] Preparation of thiol-modified gold nanoparticles, i.e. probe 1, and fingerprinting method-(2) Preparation of gold nanoparticles modified with octadecylthiol and fingerprinting method: except that octadecylthiol is used instead of dodecane Except for dodecyl mercaptan, the rest of the preparation process is the same as the preparation process of gold nanoparticles protected by dodecyl mercaptan;

[0042] Fingerprint development process: the same as gold nanoparticles functionalized with dodecyl mercaptan (attached figure 2 ).

Embodiment 3

[0044] Preparation of hydrophobized colloidal gold modified by surfactant CTAB, that is, probe 2, and fingerprint development process:

[0045] Preparation: (1) At room temperature, mix 2mM hexadecyltrimethylammonium bromide toluene solution with 1% chloroauric acid aqueous solution, stir vigorously for 5 minutes, then separate the toluene layer into another container (2) add the hexadecyltrimethylammonium bromide toluene solution of same concentration again in remaining aqueous auric acid chloride solution and still can make the aqueous auric acid chloride solution be transferred in the organic solvent toluene, repeat above process and can make chlorine Auric acid aqueous solution is all transferred in toluene; (3) in the toluene solution containing chloroauric acid, add 0.1M sodium borohydride ice water solution dropwise under stirring, until the yellow toluene solution turns red, obtains the hydrophobicity of CTAB protection. Colloidal gold (probe 2).

[0046] Fingerprint ...

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Abstract

The invention belongs to the technical field of trace amount detection, in particular relates to a method that surfaces functionalized nanogold particles are used for potential fingerprint appearance. The invention provides the method that the different surface functionalized nanogold particles (probe) are used for the potential fingerprint appearance. The particles are the hydrophobixated nanogold particles decorated by alkyl hydrosulfide, the hydrophobixated nanogold particles protected by surface active agent cetane trimethyl ammonium bromide (CTAB), the water-soluble nanogold particles protected by the CTAB and the water-soluble nanogold particles protected by L-cysteine respectively. The probe and the ingredients in the residual sweat in the potential fingerprint generate the absorption and static functions or the condensation reaction, then an argentation is utilized to lead potential fingerprint samples to colorate in the argentation liquid, the nanogold particle signals for the ingredient identification in the fingerprint are magnified and the reduced argentum particles deposite at the grain position of the fingerprint samples to further present black, thereby forming the clear fingerprint image that can be observed by naked eyes. The method is simple, fast and high in sensitivity and has no harmful side effects.

Description

technical field [0001] The invention belongs to the technical field of trace detection, and in particular relates to a method for surface-functionalized nano-gold particles for displaying latent fingerprints. Background technique [0002] Fingerprints are different from person to person, remain unchanged throughout life, and leave traces on objects. Therefore, they play an important role in evidence in criminal proceedings. Scientifically and correctly discovering, extracting, and displaying identification fingerprints have important practical functions for carrying out investigation work and punishing crimes. Fingerprint identification is one of the most reliable methods for personal identification. In forensic science, in the investigation and handling of accidents, fingerprint extraction can often provide exact identification. And with the popularization of computer technology and the application of automatic fingerprint identification system, fingerprint technology is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/117G01N1/30A61B5/1172
Inventor 苏忠民柴芳王春刚兰亚乾
Owner NORTHEAST NORMAL UNIVERSITY
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