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Method for testing microbial activity of artificial wet land sewage treatment system

A sewage treatment system and microbial activity technology, applied in biochemical equipment and methods, microbial determination/inspection, electrical digital data processing, etc., to avoid incomplete extraction, simplify operation steps, and ensure health.

Active Publication Date: 2008-08-27
SHANGHAI MUNICIPAL ENG DESIGN INST GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] The purpose of the present invention is to provide a method for detecting microbial activity in constructed wetlands, which solves the above-mentioned deficiencies in the existing methods for detecting the activity of microbial electron transfer systems in constructed wetland sewage treatment systems, and achieves rapid, accurate and safe detection of the activity of microbial electron transfer systems in constructed wetlands. the goal of

Method used

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  • Method for testing microbial activity of artificial wet land sewage treatment system
  • Method for testing microbial activity of artificial wet land sewage treatment system
  • Method for testing microbial activity of artificial wet land sewage treatment system

Examples

Experimental program
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Effect test

Embodiment 1

[0061] (1) Take out the plant roots attached to microorganisms from the constructed wetland sewage treatment system, first ultrasonically vibrate them for 10 minutes, then collect the fallen biofilms, and dilute them with sterile water for later use;

[0062] (2) Clean the biological activity test tube with sterile water, dry it at (105±1)°C to constant weight, and record the mass W 1 ;

[0063] (3) Add 1 mL of microbial suspension, 1.5 mL of Tris-HCl buffer solution with a pH of 8.4 and 1 mL of INT solution with a weight concentration of 2 mg / mL in a 10 mL test tube;

[0064] (4) Place the prepared sample in a water bath shaker at (37±1)°C, and shake it in the dark for 2 hours;

[0065] (5) After the cultivation process is over, add 1mL of formaldehyde solution with a volume concentration of 37% to the test tube to terminate the enzyme reaction, then centrifuge the sample at 4000r / min for 5min, and discard the supernatant;

[0066] (6) Add 5 mL of ethanol to the sample, mix...

Embodiment 2

[0093] (1) Take out the wetland substrate with attached microorganisms from the constructed wetland sewage treatment system, first vibrate it ultrasonically for 5 minutes, then collect the fallen biofilm, and dilute it with sterile water for later use;

[0094] (2) Clean the biological activity test tube with sterile water, dry it at (105±1)°C to constant weight, and record the mass W 1 ;

[0095] (3) Add 1 mL of microbial suspension, 2 mL of Tris-HCl buffer solution with a pH of 8.4 and 2 mL of INT solution with a weight concentration of 2 mg / mL in the test tube of 10 mL;

[0096] (4) Place the prepared sample in a water bath shaker at (37±1)°C, and shake it in the dark for 1 hour;

[0097] (5) After the cultivation process is over, add 1mL of formaldehyde solution with a volume concentration of 37% to the test tube to terminate the enzyme reaction, then centrifuge the sample at 4000r / min for 5min, and discard the supernatant;

[0098] (6) Add 5 mL of ethanol to the sample,...

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Abstract

The present invention provides a microorganism activity detection method for constructed wetland sewage treatment system, comprising the following basic steps of constructed wetland microorganism sampling, cultivation, extraction, measurement and calculation. The present invention adopts iodine nitro group tetrazolium as an electron acceptor, cancels oxygen removal step on the basis of the strongelectron affinity of INT, adopts 2h as the cultivation time, and uses ethanol as extractant to extract INTF inside microorganism cells. The detection method has the advantages that: the INT is strongin electron affinity and the ability of competing with oxygen for electrons; the present invention can be used to detect the electron transport system activity of aerobic, anaerobic and denitrification nitrogen removal microorganisms in constructed wetland systems, finishes extraction process under the condition of normal temperature, and avoids incomplete extraction, extractant evaporation and other problems which are caused by high temperature; the microorganism dry weight direct determination method is combined with the detection process of microorganism electron transport activity, thereby avoiding errors caused by different matrixes and plant rhizosphere biomass as well as uneven sampling.

Description

technical field [0001] The invention relates to a method for detecting microbial activity, in particular to a method for detecting microbial activity in a sewage treatment system. Background technique [0002] The metabolic activity of microorganisms in the constructed wetland sewage treatment system is the key to maintaining the long-term purification effect of the constructed wetland, and microorganisms play an important role in the process of organic matter degradation and denitrification. [0003] Microbial activity is an important index to characterize the strength of microbial metabolism in constructed wetland system. This index can not only reflect the ability of microorganisms to remove pollutants in constructed wetland sewage treatment system from the perspective of molecular biology, but also measure the biological activity of pollutants from the level of biological activity. Degradation rate and evaluation and prediction of operating effect of constructed wetland ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/17G06F19/00C12Q1/04
Inventor 谭学军张辰唐利王国华
Owner SHANGHAI MUNICIPAL ENG DESIGN INST GRP
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