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In-vitro propagation method of tuberose virus

A technology of in vitro reproduction and tuberose, applied in the field of in vitro reproduction of tuberose virus, can solve the problems of labor-consuming, occupying greenhouse space, etc., so as to improve work efficiency, maintain infectivity, simplify reproduction and preserve virus. The effect of the source step

Inactive Publication Date: 2011-06-15
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Not only consume a lot of labor, but also take up a lot of greenhouse space

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1), the preparation of the culture medium, including each component of the basic culture medium and the culture medium of each stage of tissue culture and the weight per liter are:

[0047] (1) Basic medium: MS medium for induction, proliferation and growth medium; 1 / 2MS medium for rooting medium; wherein, agar 8g / L, pH5.8;

[0048] (2) Induction medium: MS+BA 2.0mg / L and IAA 2.0mg / L+sucrose 30g / L;

[0049] (3) Proliferation medium: MS+BA 1.0mg / L and IAA 1.0mg / L+sugar 30g / L;

[0050] (4) Growth medium: MS+BA 0.5mg / L and IAA 0.5mg / L+sugar 40g / L;

[0051] (5) Rooting medium: 1 / 2MS+IBA 1.0mg / L and IAA 0.25mg / L+white sugar 20g / L.

[0052] 2), tissue culture of poison source tuberose:

[0053] (1) Selection and sterilization of explants: select poison source bulbs, peel off the bulb epidermis and rinse with tap water, after sterilization, under aseptic conditions, cut young shoots as explants for tissue culture;

[0054] (2) Induction culture: the young shoots are inocul...

Embodiment 2

[0074] In this example, the agar 7g / L of its basic medium, pH5.6; Induction medium: MS+BA 1.0mg / L and IAA 1.0mg / L+sucrose 30g / L; Proliferation medium: MS+BA 0.5mg / L L and IAA 0.5mg / L+sugar 30g / L; growth medium: MS+BA 0.1mg / L and IAA 0.1mg / L+sugar 40g / L; rooting medium: 1 / 2MS+IBA 0.5mg / L and IAA0. 1mg / L+white sugar 20g / L. The young shoots were cut out, soaked in 75% alcohol for 0.5 min, then soaked in 0.1% mercuric chloride aqueous solution for 10 min, and finally rinsed with sterile water for 3 to 5 times for sterilization, and used as explant materials for tissue culture. All the other steps and conditions are the same as in Example 1.

Embodiment 3

[0076] In this example, the agar 9g / L of its basic medium, pH6.0; Induction medium: MS+BA 3.0mg / L and IAA 3.0mg / L+sucrose 30g / L; Proliferation medium: MS+BA 2.0mg / L L and IAA 2.0mg / L+sugar 30g / L; growth medium: MS+BA 1.0mg / L and IAA 1.0mg / L+sugar 40g / L; rooting medium: 1 / 2MS+IBA 2.0mg / L and IAA0. 5mg / L+ white sugar 20g / L. Cut the bulbs into 0.5cm×0.5cm×0.5cm cubes, soak in 75% alcohol for 1.0min, then soak in 0.1% mercuric chloride aqueous solution for 15min, and finally rinse with sterile water for 3 to 5 times for sterilization. As a material for explants for tissue culture. All the other steps and conditions are the same as in Example 1.

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Abstract

The invention relates to a method for propagating tuberose virus in vitro, which belongs to the technical field of plant virus propagation and comprises following steps: preparing culture medium, culturing tissues of a drug source tuberose, checking virus, and propagating the drug source tuberose virus in vitro. The method of the invention has the advantages that a plant tissue culture technique is utilized to propagate the tuberose virus in vitro, the tuberose virus can be propagated without limit, and the original infecting potential is maintained, which not only solves the problem that thedrug source is captured in imported virus disease quarantine, but also greatly simplifies the steps of propagation and drug source storage, the work for seeding differential hosts repeatedly, for reviving the drug source repeatedly, for transferring repeatedly, and for disinfecting soil before seeding to store some drug source is saved, the working efficiency is increased in times, and infectioustiter is easily extracted and prepared, which provides the virus with high purity for preparing virus detection antiserum.

Description

technical field [0001] The invention relates to the technical field of in vitro propagation of plant viruses, in particular to a method for in vitro propagation of tuberose virus. Background technique [0002] Tuberose (Polianthes tuberosa L.) was originally a plant of Amaryllidaceae wild in Mexico and South America, and now it is cultivated all over the world. Its cut flowers and essential oils have high economic value. Since it was introduced to China more than 300 years ago, it has become an important ornamental plant, especially in Taiwan. The occurrence of tuberose virus disease is very common. The existence of the virus in the tuberose plant makes the growth of the tuberose plant slow, the number of inflorescences per plant, the flower seat rate decrease, the flowering period is shortened, the flowers are small and the color is light, and the commodity The value is greatly reduced. [0003] Zhejiang Academy of Agricultural Sciences found mild mottled symptoms from Ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00C12N7/02
Inventor 陈剑平徐刚汪一婷吕永平牟豪杰郑红英林林
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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