Method for extracting taurine from sea cucumber sausage

Abstract

A method for extracting taurine from sea cucumber intestines, which is characterized in that it consists of the following steps: (1) heating and extracting sea cucumber intestines in water, and collecting the sea cucumber intestine extract; (2) filtering the sea cucumber intestine extract through plate and frame or The supernatant is obtained by centrifugation in a decanter centrifuge, a disc centrifuge and a tube centrifuge in sequence; (3) The supernatant is electrodialyzed by an electric drive device, desalinated, and the conductance reaches 300-1000μS / cm to stop, and the desalinated supernatant is obtained. (4) The desalted clear liquid is ultrafiltered with a membrane with a molecular weight of 1000Da, and the filtrate is collected; (5) After the filtrate is concentrated under reduced pressure for 20-40 times the volume, ethanol with a concentration of more than 95% is added to make the final concentration When it reaches 50-60%, let it stand at 4°C, and collect the precipitate by suction filtration or centrifugation. (6) After the precipitate is dissolved in water, add ethanol with a concentration of more than 95% to make the final concentration reach 50-60%, carry out recrystallization, repeat this step once, and carry out recrystallization again to obtain pure taurine.

Description

technical field [0001] The invention relates to a method for extracting taurine from sea cucumber intestines. Background technique [0002] Sea cucumber sausage is a by-product resource produced in the process of fresh sea cucumber processing. Because it contains a large amount of sediment, it is generally used to remove the sediment manually, but it is difficult to use it on a large scale. Studies have reported that sea cucumber intestines contain not only proteins, polysaccharides, and saponins, but also a large amount of taurine (Analysis and comprehensive evaluation of nutritional components in different parts of sea cucumbers, Food Industry Science and Technology, Volume 31, Issue 5, 2010 ). [0003] Taurine has many physiological functions and is an essential nutrient for human health. There are many reports on the extraction of taurine from marine organisms, such as extracting taurine from oysters, the usual method is as follows: fresh oysters→washing away the sedim...

AI Technical Summary

Problems solved by technology

[0002] Sea cucumber sausage is a by-product resource produced during the processing of fresh sea cucumber. Because it contains a large amount of sediment, it is generally used to remove the sediment manually, but it is difficult to use it on a large scale

At present, there is no literature report on extracting taurine from sea cucumber intestines