Use of safflower loranthus parasiticus polysaccharides in pharmaceuticals

A technology for polysaccharide extraction and mulberry parasitism, which is applied in solid solvent extraction, semi-permeable membrane separation, chemical instruments and methods, etc., and can solve the problems of poor water solubility, low yield and unfavorable application of polysaccharide substances.

Inactive Publication Date: 2010-06-02
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In our research, we found that using the traditional method to extract polysaccharides from the safflower mulberry parasite requires multiple use of precipitating agents to precipitate polysaccharides from the solution, which consumes a large amount of solvent and has a low yield. The extracted polysaccharides have poor water solubility. Not conducive to application

Method used

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  • Use of safflower loranthus parasiticus polysaccharides in pharmaceuticals
  • Use of safflower loranthus parasiticus polysaccharides in pharmaceuticals
  • Use of safflower loranthus parasiticus polysaccharides in pharmaceuticals

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Take 180g of crushed medicinal material powder, add 80% ethanol to reflux and extract for 3 times, each time for 2 hours, take the filter residue by suction filtration, evaporate the solvent, add water according to the ratio of material to liquid 1:10, extract in hot water at 95°C, each time for 2 hours, Extracted 3 times in total, combined the 3 extracts, filtered to remove the residue, concentrated the filtrate under reduced pressure at 60°C to a volume of about 1000ml, added 1 / 3 volume of sevage (chloroform:n-butanol=4:1), and oscillated for 20min Afterwards, centrifuge at 8000rpm for 15min to remove the protein precipitate, take the supernatant and repeat it several times until there is no protein. The deproteinized solution was put into a dialysis bag for dialysis, running water dialysis for 2 days, distilled water dialysis for 1 day. Concentrate under reduced pressure to 1 / 2 volume, add 3 times the amount of 95% ethanol, let it stand in the refrigerator for 24 hou...

Embodiment 2

[0024] Take 180g of crushed medicinal material powder, add 80% ethanol to reflux and extract for 3 times, each time for 2 hours, take the filter residue by suction filtration, evaporate the solvent, add water according to the ratio of material to liquid 1:10, extract in hot water at 95°C, each time for 2 hours, Extract 3 times, combine 3 extracts, remove residue by suction filtration, add 0.5% papain (w / v) to the filtrate, enzymatically hydrolyze at 40°C for 5h, heat to 100°C for 10min to inactivate enzyme activity, concentrate under reduced pressure at 60°C To about 1000ml volume, add 1 / 3 volume of sevage (chloroform:n-butanol=4:1), shake for 20min, centrifuge at 8000rpm for 15min, repeat 3 times. The deproteinized solution was put into a dialysis bag for dialysis, running water dialysis for 2 days, distilled water dialysis for 1 day. Concentrate to 1 / 2 volume, add 3 times the amount of 95% ethanol, put it in the refrigerator for 24 hours to precipitate, centrifuge to collect...

Embodiment 3

[0026] Take 180g of crushed medicinal material powder, add 80% ethanol to reflux and extract for 3 times, each time for 2 hours, take the filter residue by suction filtration, evaporate the solvent, add water according to the ratio of material to liquid 1:10, extract in hot water at 95°C, each time for 2 hours, Extracted 3 times in total, combined 3 extracts, filtered to remove residue, concentrated the filtrate under reduced pressure at 60°C to a volume of about 1000ml, added an equal volume of 30% trichloroacetic acid, oscillated for 20min, then stood at 4°C for 2h, 8000rpm Centrifuge for 15 minutes to remove protein precipitates. The deproteinized solution was put into a dialysis bag for dialysis, running water dialysis for 2 days, distilled water dialysis for 1 day. Concentrate to 1 / 2 volume, add 3 times the amount of 95% ethanol, put it in the refrigerator for 24 hours to precipitate, centrifuge to collect the precipitate, wash with absolute ethanol, acetone, ether in tur...

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Abstract

The invention relates to an extraction method of plant polysaccharide, in particular to an extraction method of scurrula parasitica polysaccharide, which comprises breaking scurrula parasitica leaf, extracting via alcohol, filtering and taking slags, immerging and extracting slags via hot water, removing protein and small molecules to obtain scurrula parasitica polysaccharide. The invention is characterized in that the supernatant extracted by hot water immersion extraction method is treated by sevage method, TCA method and enzyme method or the combination to remove protein and dialyze to remove small molecules. The inventive polysaccharide extraction method only uses precipitant for one time to deposit polysaccharide, thereby saving solvent and improving the polysaccharide yield and solubility. The scurrula parasitica polysaccharide has strong inhibition on mice sarcoma S180 and can prolong the service life H22 mice of hepatic cancer ascites tumor, while the cancer inhibition is not depended on dosage.

Description

technical field [0001] The invention relates to an extraction method and medical use of an extract of Scurrula parasitica L., in particular to the use of a polysaccharide extract of Scurrula parasitica L. in preparing antitumor drugs. Background technique [0002] The safflower mulberry Scurrula parasitica L. is a semi-parasitic plant of the genus Pyroparasitica of the subfamily Morusaceae. It is a unique plant in my country and is mainly distributed in southern regions such as Guangdong, Guangxi, Yunnan and Fujian. Traditionally, safflower mulberry is often used as a substitute for mulberry tree Taxillus chinensis (DC.) Danser. It has the functions of nourishing liver and kidney, dispelling rheumatism, lowering blood pressure, calming blood and nourishing fetus, etc. It is mainly used for rheumatism, joint pain, high blood pressure, sciatic nerve Pain, low back pain, postpartum milk loss embolism. [0003] Plants of the family Morushiaceae generally contain a large amount ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00B01D11/02B01D61/00
Inventor 肖义军陈元仲范延丽
Owner FUJIAN NORMAL UNIV
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