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Horse bean root tip cell microkernel flaking method

A root tip and broad bean technology, which is applied in the field of micronucleus production of broad bean root tip cells, can solve the problems of restricting wide application, achieve the effect of shortening the test time, clear cell outline, and ensuring feasibility and accuracy

Inactive Publication Date: 2010-08-18
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Judging from the currently used production methods, the above-mentioned deficiencies do exist, which to a certain extent limits the wide application of micronucleus technology in the root tip of faba beans in environmental pollution monitoring and mutagen detection.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] (1) Seed soaking to accelerate germination: Select broad bean seeds without pests and diseases and soak them in warm water at 25°C. The germination culture is carried out in a constant temperature incubator at ℃, and the water should be changed frequently during the cultivation period. When the roots grow to 1-2 cm, the seeds with good root growth and consistent length are selected for testing;

[0011] (2) Preparation of fixed eluent: first take absolute ethanol and glacial acetic acid at a volume ratio of 125:3, and mix them to make a stationary solution. Mixed with the eluent to prepare a fixed eluent;

[0012] (3) Fixed segregation: Take a root tip about 1 cm long and place it in a fixed segregation solution at 28°C for 5 minutes, then take out the root tip and wash it with distilled water for 2 to 3 times, each time for 2 to 3 minutes, until the fixed segregation on the root tip liquid cleaning;

[0013] (4) Staining and pressing: place the cleaned root tip on a ...

Embodiment 2

[0015] (1) Seed soaking to accelerate germination: Select broad bean seeds without pests and diseases and soak them in warm water at 25°C. Carry out germination cultivation in a constant temperature box at ℃, change the water frequently during the cultivation period, and when the roots grow to 1-2 cm, select seeds with good root growth and consistent length for experimentation;

[0016] (2) Preparation of fixed eluent: first take absolute ethanol and glacial acetic acid at a volume ratio of 125:3, and mix them to make a stationary solution. The eluent and the eluent are mixed to prepare a fixed eluent;

[0017] (3) Take a root tip of about 1 cm and put it in the fixed educt solution at 29°C for 6 minutes, then take out the root tip and wash it with distilled water for 2 to 3 times, each time for 2 to 3 minutes, until the fixed educt solution on the root tip is cleaned ;

[0018] (4) Staining and pressing: put the cleaned root tip on a glass slide, blot the water, take about ...

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PUM

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Abstract

The present invention discloses a method for preparing the piece of root apiex microkernel of lima beans, the method comprises the steps of soaking seed to hasten germination to the seed of the lima beans, fixed-isolating the root tip, dyeing and performing and the like. The method integrates the fixing and the isolating to one step with a fixed-isolating liquid, lead to that the experiment whichneeds about 25 hours for completion formerly can be completed in about 30 minutes, the experiment time is greatly saved and the chemical consumption is reduced; and through the using of the cell nucleus dye liquor with simple operation and good dyeing effect the optimum microkernel piece-making effects of evident cell nucleus dyeing, large nuclearsubstance contrast ratio and definite cell profileare obtained thereby ensuring the feasibility for monitoring the environment with the microkernel technique of the lima beans root tip and the accuracy.

Description

technical field [0001] The invention relates to a cell micronucleus preparation method, in particular to a faba bean root tip cell micronucleus preparation method. Background technique [0002] Plant broad bean (Vicia faba L) root tip cells have large chromosomes, few in number, rich in DNA, and sensitive to mutagen. Broad bean root tip cell micronucleus technology was established by Francesca and MaTe-Hsiu in 1982, and it has been adopted by China, the United States, the United Nations Environment Program (UNEPA) and the World Health Organization (WHO) in environmental pollution monitoring and mutagen detection. It is recognized by many countries, regions and international organizations. In the "Water and Wastewater Monitoring and Analysis Methods" promulgated by the State Environmental Protection Administration of China in 2002, the broad bean root tip micronucleus test was listed as a B-type method used in the environmental monitoring and law enforcement process, and in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
Inventor 王跃华孙艳郑奕孙雁霞马丹炜徐文俊邬晓勇刘碧崇
Owner CHENGDU UNIV
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