Method for extracting proanthocyanidins from cranberry

A technology of proanthocyanidins and cranberry, applied in the direction of organic chemistry and the like, can solve the problems of high cost and complicated process, and achieve the effects of low production cost, easy industrialization and simple process flow

Inactive Publication Date: 2008-08-13
上海华珠生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the extraction and separation of proanthocyanidins for lingonberry plants, especially cranberries, and the existing ones have to go through a complicated separation and extraction process.
As described in the invention patent (Patent No. 96192723.2) and the literature (Phytochemistry, 2005 (66): 2281.), the former uses cranberry fruit powder to separate proanthocyanidins, which requires a cumbersome raw material processing process, and both of them need to go through two column layers analysis, high cost and complicated process

Method used

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  • Method for extracting proanthocyanidins from cranberry
  • Method for extracting proanthocyanidins from cranberry

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1 The method for extracting proanthocyanidins from cranberries

[0033] 1. Raw material processing:

[0034] Grind 200 grams of cranberries in 50 ml of solvent at 4°C for 5 minutes (solvent is: 70% acetone by volume, 30% methanol by volume, 0.1g / 100ml citric acid), get the supernatant after centrifugation, and remove the pulp , and then the solvent was evaporated to dryness, and the extract was dissolved in water to obtain a cranberry extract.

[0035] 2. Solvent degreasing:

[0036] Add an equal volume of petroleum ether to the cranberry extract for degreasing treatment, repeat once for 3 hours each time. Evaporate and dry the degreased cranberry extract under reduced pressure to obtain cranberry extract.

[0037] 3. Sephadex LH-20 gel column chromatography separation and purification:

[0038] The LH-20 column (purchased from Pharmacia Company) was equilibrated overnight with 50% ethanol aqueous solution by volume percentage; the cranberry extract was d...

Embodiment 2

[0050] Embodiment 2 The method for extracting proanthocyanidins from cranberries

[0051] 1. Raw material processing:

[0052] 200 grams of cranberries were ground for 5 minutes at 25°C in 100ml of solvent (solvent: 70% acetone by volume, 30% methanol by volume, 0.1g / 100ml citric acid), and the pulp was removed by sieve filtration, and then the solvent Evaporate to dryness, and dissolve the extract in water to obtain a cranberry extract.

[0053] 2. Solvent degreasing:

[0054] Add an equal volume of petroleum ether to the cranberry extract for degreasing treatment, and repeat twice for 1 hour each time. Evaporate and dry the degreased cranberry extract under reduced pressure to obtain cranberry extract.

[0055] 3. Sephadex LH-20 gel chromatography:

[0056] The LH-20 column (purchased from Pharmacia Company) was equilibrated overnight with 50% ethanol by volume percentage, and the cranberry extract was dissolved in 10ml volume percentage 50% ethanol and loaded immediately ...

Embodiment 3

[0057] Embodiment 3 The method for extracting proanthocyanidins from cranberries

[0058] 1. Raw material processing:

[0059] Dilute 10 ml of cranberry juice concentrate (commercially available) to 25 ml with water to obtain a cranberry extract.

[0060] 2. Solvent degreasing:

[0061] Add an equal volume of petroleum ether to the cranberry extract for degreasing treatment, repeat once, 1 hour each time. Evaporate and dry the degreased cranberry extract under reduced pressure to obtain cranberry extract.

[0062] 3. Sephadex LH-20 gel chromatography:

[0063] The LH-20 column (purchased from Pharmacia Company) was equilibrated overnight with 50% ethanol by volume, and the cranberry extract was dissolved in 10ml 50% ethanol by volume, and loaded immediately after vortexing (10 grams of LH-20 gel, The column volume is 40ml); wash the column with 50% ethanol by volume, and the flow rate is 2.5ml / min, until the eluate that flows out becomes colorless, discard the washing solu...

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Abstract

The invention relates to a method for extracting proanthocyanidin from cranberryin, comprising: treating material to obtain cranberryin extracting liquid; performing degreasing; and separating and purifying by using column chromatography. The method of the invention is simple in process, low in cost, easy in industrialization.

Description

technical field [0001] The invention relates to a method for extracting proanthocyanidins from cranberries. Background technique [0002] Proanthocyanidins (PACs) is a general term for a large class of polyphenolic compounds that widely exist in the plant kingdom. At first, they were collectively attributed to condensed tannins or flavanols. With the improvement of separation and identification technology and in-depth research on such substances, Has now become a unique category of substances, and called proanthocyanidins. Proanthocyanidins (PACs) are polymers of flavan-3-ols (flavan-3-ols) or flavan-3,4-diols, also known as condensed tannins. That is to say, it is a polymer formed by linking flavan monomers through C-C bond and C-O ester bond. In edible plants, flavan monomers mainly pass through B-type linkage (B-type linkage) [C4-C8, and C4-C6], and A-type linkage (A-type linkage) [C4-C8 And C2-O-C7] mode connection polymerization, the degree of polymerization can be d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C07D311/22
Inventor 史彤高飞
Owner 上海华珠生物科技有限公司
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