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Industrial fermentation ramee rapid extraction technique for Erwinia

A technology of Erwinia and ramie, which is applied in the field of ramie biological degumming, can solve the problems of difficulty in guaranteeing activity, high probability of contamination by bacteria, and high transportation costs, and achieve the effects of reducing the loss of effective fibers, shortening the fermentation cycle, and reducing technical difficulty

Active Publication Date: 2010-07-21
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The current hemp degumming and grass pulping enterprises all use chemical methods. There are three outstanding problems in chemical degumming or pulping methods: one is high cost and high energy consumption, two is a lot of fiber loss and structure damage, and the third is environmental pollution serious
The enzyme preparation production workshop was built in the ramie processing enterprise. Due to the lack of relevant professionals, the long production cycle of enzyme preparation (more than 100h), and the high probability of contamination by bacteria, the company's degumming production stability was poor; the enzyme preparation production workshop was moved to In other places, if the crude enzyme solution is used for ramie degumming, the activity is difficult to guarantee and the transportation cost is high. If the refined enzyme is used, the production cost of the enzyme preparation will exceed the bottom line that the current enterprise can bear

Method used

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Experimental program
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Effect test

Embodiment Construction

[0024] (1) Breeding of Erwinia mutant strain CGMCC2194

[0025] 1. Implementation location

[0026] The Engineering Microbiology Laboratory of the Hemp Research Institute of the Chinese Academy of Agricultural Sciences (an important part of the Key Open Laboratory of Hemp Genetic Improvement and Engineering Microorganisms of the Ministry of Agriculture).

[0027] 2. Research on strain selection and breeding

[0028] Using the T85-260 (CGMCC0245) strain as the originating bacteria, irradiate it with ultraviolet light for 120s, and then, according to the routine microbial selection procedures and methods, the conventional microbial strain enzyme production performance identification and the laboratory's original ramie degumming strain selection CGMCC2194, a mutant strain of Erwinia, was isolated, purified, identified and screened from the ultraviolet mutagenized bacteria solution.

[0029] (2) Study on the biological characteristics of Erwinia mutant strain CGMCC2194 and the p...

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Abstract

The invention discloses a technique for rapidly extracting ramie hemp erwinia bacterium factory production fermentation, the technique comprises preprocessing material, immersing or sprinkling and inoculating, immersing or fermenting, deactivating with hot water, and the procedure of circulation washing, water dressing and fiber finish, erwinia bacterium which has broad-spectrum and high effective to degumming, pulping and saccharifying of herb fiber material, and bacterial agent preparation and activated technical parameter. The erwinia bacterium addicts the mannose, the peak for secreting non-cellulose to degrade key enzyme (pectic enzyme, beta-mannase and xylanase) can be achieved through purely culturing for 7h to 9h. Bacterial agent is inoculated on herb fiber material such as ramie and the like for activating the bacterial agent for 5h-6h, fermentation forage fodder turns to blue after fermenting for 5h-6h, pectine, hemicellulose and partial lignin which are partially degraded and associated in fiber cell are stripped, non-cellulose residues which are absorbed on fiber can be removed by means of the flushing of high-pressure flushing, and the purpose of extracting net fiber can be reached.

Description

technical field [0001] The invention belongs to the field of ramie biological degumming in the textile industry, and in particular relates to an Erwinia bacillus and a process for extracting ramie fibers. Background technique [0002] The current hemp degumming and grass pulping enterprises all use chemical methods. There are three outstanding problems in chemical degumming or pulping methods: one is high cost and high energy consumption, two is a lot of fiber loss and structure damage, and the third is environmental pollution serious. In particular, chemical methods damage the inherent morphology and structure of fibers while degrading non-cellulose, making fiber products lose many excellent properties. These problems have already become the restrictive factors of industrial development. In order to solve the problems existing in traditional and conventional methods, extensive researches on "biological degumming", "biological pulping" and "biological saccharification" hav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N15/01D01C1/04C12S3/06C12R1/18
Inventor 刘正初彭源德冯湘沅郑科段盛文胡镇修
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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