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Total alkaloid of stephania delavayi and preparation and application thereof

A technology of total alkaloids and jinbuhuan, which is applied in the field of medicine, can solve the problems of pharmacological effects and clinical application of total alkaloids of jinbuhuan, and achieve the effect of reducing the incidence of dysmenorrhea and shrinkage

Active Publication Date: 2011-05-11
GUANGZHOU BOJI MEDICINE SERVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] By analyzing the chemical components of Jinbuhuan medicinal material and the pharmacological effects of individual components, the inventors consulted domestic and foreign literature, and found no reports on the pharmacological effects and clinical application of Jinbuhuan total alkaloids.

Method used

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  • Total alkaloid of stephania delavayi and preparation and application thereof
  • Total alkaloid of stephania delavayi and preparation and application thereof
  • Total alkaloid of stephania delavayi and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Take 1.0Kg of the coarse powder of Jinbuchang medicinal material, add 12 times the amount of 75% ethanol to percolate at a speed of 30ml / min / Kg medicinal material, and collect the percolation liquid. Recover ethanol under reduced pressure, concentrate to a thick paste (relative density 1.30, 50°C), dissolve with 5% sulfuric acid to 1000ml, filter, pass through D101 macroporous resin column for adsorption, wash with water until neutral, then elute with 95% ethanol, The eluate was collected, ethanol was recovered under reduced pressure, and vacuum-dried to obtain 55 g of total alkaloids of Jinbuhuan, which contained 75.8% of total alkaloids in terms of tetrahydropalmatine.

Embodiment 2

[0034] Take 1Kg of the coarse powder of the medicinal material of Jinbuchang, add 16 times the amount of 95% ethanol to percolate at a rate of 20ml / min / Kg of the medicinal material, and collect the percolation liquid. Recover ethanol under reduced pressure, concentrate to a relative density of 1.28 (50°C), dissolve with 5% sulfuric acid solution, filter, adjust the pH value of the filtrate to 9-11 with ammonia water, let it stand for 24 hours, filter, take the filter residue, and wash with water until Neutral, and then dissolved with 12 times the weight of 95% ethanol, recovered the ethanol under reduced pressure, and dried in vacuo to obtain 48g of total alkaloids of Jinbuhuan, containing 68.2% of total alkaloids in terms of tetrahydropalmatine.

Embodiment 3

[0036] Take 1Kg of Jinbuhuan medicinal material coarse powder, add 10 times the amount of 80% ethanol to reflux twice, filter, combine the ethanol liquid, recover ethanol under reduced pressure, concentrate to a thick paste (relative density 1.30, 50°C), dissolve with 0.5% sulfuric acid 8000ml , filtered, adsorbed on a strong acidic cation exchange resin column, washed with water to remove impurities, took out the resin, added 2 times the amount of 5% ammonia water and ethanol to reflux extraction three times, each time for 1.5 hours, the extract was concentrated under reduced pressure, vacuum dried, and the total biological Alkali 40g, containing total alkaloids is 86.1% in terms of tetrahydropalmatine.

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PUM

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Abstract

The invention discloses a total alkaloid of Stephania delavayi Diels, the content of which is measured by tetrahydropalmatine. Tetrahydropalmatine occupies 55-95 percent of the total alkaloid extract of Stephania delavayi Diels, wherein the weight percentage of tetrahydropalmatine contained is no less than 2 percent, while the weight percentage of palmatine hydrochloride contained is no less than0.8 percent. The invention also discloses a preparation method of the total alkaloid of Stephania delavayi Diels, which relates to the steps as follows: adding the Stephania delavayi Diels meal into ethanol for diacolation or reflux to acquire alcohol extract, decompressing and recycling the ethanol and concentrating into a condensed ointment for dissolving and filtrating with acid to acquire a filtrate, passing the filtrate through a macroporous resin column or a strong acid cation exchange resin column for adsorption, washing with water to remove the impurities and extracting with an eluent, collecting the eluent and decompressing and recycling the extract and then concentrating and drying, thus acquiring the total alkaloid of Stephania delavayi Diels; the steps can also be decompressing a percolating liquid to recycle the ethanol, concentrating, dissolving with acid and then filtrating, adjusting the pH value of the filtrate with weak base to produce deposition, filtrating the deposition to acquire a filter residue, adding ethanol into the filter residue to resolve and decompress, recycle the ethanol and then drying, thus acquiring the total alkaloid. The results of a pharmacological study on the acquired total alkaloid show that the total alkaloid of Stephania delavayi Diels has good effect on spasmolysis, pain relieving, antiphlogosis and antibiosis, blood activating and so on, and can combine with the acceptable components in pharmacy to prepare various sustained release or controlled release formulation or other formulations.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a medicinal material extract of Jinbuhuan-Jinbuhuan total alkaloids, and meanwhile, the invention also relates to a preparation method and application of Jinbuhuan total alkaloids. Background technique [0002] Jin Buhuan is the dry tuber root of the Fangheke plant that does not tolerate Stephania kwangsiensis H.S.Lo in Guangxi, Stephania micrantha H.S.Lo et M.Yang in Xiaohuadi or Stephania kuinanensis H.S.Lo et M.Yang in Southern Guangxi. Function Clearing heat and detoxifying, dissipating stasis and reducing swelling, invigorating the stomach and relieving pain; used for stomach and duodenal ulcer pain, upper respiratory tract infection, acute gastroenteritis, bacillary dysentery, toothache, neuralgia, carbuncle sore, swelling and pain from bruises . [0003] Studies have shown that Jinbuhuan medicinal materials mainly contain alkaloids, mainly tetrahydropalmaine,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/59A61K31/4745A61K9/20A61K9/48A61K9/70A61K9/08A61K9/16A61K9/22A61K47/38A61P1/04A61P19/02A61P15/00
Inventor 马仁强陈健文邹红
Owner GUANGZHOU BOJI MEDICINE SERVICES
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