Total alkaloid of stephania delavayi and preparation and application thereof
A technology of total alkaloids and jinbuhuan, which is applied in the field of medicine, can solve the problems of pharmacological effects and clinical application of total alkaloids of jinbuhuan, and achieve the effect of reducing the incidence of dysmenorrhea and shrinkage
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Embodiment 1
[0032] Take 1.0Kg of the coarse powder of Jinbuchang medicinal material, add 12 times the amount of 75% ethanol to percolate at a speed of 30ml / min / Kg medicinal material, and collect the percolation liquid. Recover ethanol under reduced pressure, concentrate to a thick paste (relative density 1.30, 50°C), dissolve with 5% sulfuric acid to 1000ml, filter, pass through D101 macroporous resin column for adsorption, wash with water until neutral, then elute with 95% ethanol, The eluate was collected, ethanol was recovered under reduced pressure, and vacuum-dried to obtain 55 g of total alkaloids of Jinbuhuan, which contained 75.8% of total alkaloids in terms of tetrahydropalmatine.
Embodiment 2
[0034] Take 1Kg of the coarse powder of the medicinal material of Jinbuchang, add 16 times the amount of 95% ethanol to percolate at a rate of 20ml / min / Kg of the medicinal material, and collect the percolation liquid. Recover ethanol under reduced pressure, concentrate to a relative density of 1.28 (50°C), dissolve with 5% sulfuric acid solution, filter, adjust the pH value of the filtrate to 9-11 with ammonia water, let it stand for 24 hours, filter, take the filter residue, and wash with water until Neutral, and then dissolved with 12 times the weight of 95% ethanol, recovered the ethanol under reduced pressure, and dried in vacuo to obtain 48g of total alkaloids of Jinbuhuan, containing 68.2% of total alkaloids in terms of tetrahydropalmatine.
Embodiment 3
[0036] Take 1Kg of Jinbuhuan medicinal material coarse powder, add 10 times the amount of 80% ethanol to reflux twice, filter, combine the ethanol liquid, recover ethanol under reduced pressure, concentrate to a thick paste (relative density 1.30, 50°C), dissolve with 0.5% sulfuric acid 8000ml , filtered, adsorbed on a strong acidic cation exchange resin column, washed with water to remove impurities, took out the resin, added 2 times the amount of 5% ammonia water and ethanol to reflux extraction three times, each time for 1.5 hours, the extract was concentrated under reduced pressure, vacuum dried, and the total biological Alkali 40g, containing total alkaloids is 86.1% in terms of tetrahydropalmatine.
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