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Method for preparing powdered phosphatidyl serine

A powdered phosphatidylserine and serine technology, which is applied in the field of compound preparation, can solve the problems of high cost, large investment in equipment, and difficult to guarantee the reaction conversion rate, etc., and achieve the effect of low cost, small investment in equipment, and reduction of by-products.

Inactive Publication Date: 2008-07-30
杨凌萃健生物工程技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention provides a method for preparing powdered phosphatidylserine to overcome the problems of large equipment investment, high cost and difficult guarantee of reaction conversion rate in the prior art

Method used

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  • Method for preparing powdered phosphatidyl serine

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Experimental program
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Effect test

Embodiment 1

[0020] Weigh 50 g of soybean lecithin with a phosphatidylcholine (PC) content of 25.32% (powdered, commercially available). Add 400ml of water and stir, heat at 50°C, and disperse evenly after half an hour. In addition, weigh 100g of L-serine, CaCL 2 3.2g, 12g sodium acetate, 1.4ml acetic acid, add 400ml water and stir to dissolve, the measured pH is 5.0, after mixing the two systems, add PLD0.18g, the activity unit is 1000units / g. Start timing, the reaction temperature is 40° C. to 50° C., and the conversion rate is detected after 18 hours of reaction. After complete conversion, add ethyl acetate for extraction, then concentrate the extract, add 0.5mg VE as an antioxidant, and finally treat the mixed phospholipids with acetonitrile to obtain 26.3g powdered PS with a PS content of 43.78%. See Schedule.

Embodiment 2

[0022] Weigh 50g of soybean lecithin with a phosphatidylcholine (PC) content of 48.73% (granular, commercially available), add 300ml of water and stir, heat at 20°C, and disperse evenly after half an hour. In addition, weigh 200g of L-serine, CaCL 2 6.4g, 22.2g sodium acetate, 2.8ml acetic acid, add 300ml water and stir to dissolve, the measured pH is 5.0, after mixing the two systems, add 0.80g PLD, the activity unit is 1500units / g. Start timing, the reaction temperature is 40° C. to 50° C., and the conversion rate is detected after 20 hours of reaction. After complete conversion, add n-hexane for extraction, then concentrate the extract, add 2.5 mg VC as an antioxidant, and finally treat the mixed phospholipids with ethanol to obtain 43.0 g powdered PS with a PS content of 53.68%.

Embodiment 3

[0024] Weigh 50g of soybean lecithin with a phosphatidylcholine (PC) content of 60.30% (block, commercially available), add 400ml of water and stir, heat at 50°C, and disperse evenly after half an hour. In addition, weigh 300g of L-serine, CaCL 27.9g, 27.4g sodium acetate, 3.5ml acetic acid, add 400ml water and stir to dissolve, the measured pH is 5.2, after mixing the two systems, add 1.10g PLD, the activity unit is 1500units / g. Start timing, the reaction temperature is 40° C. to 50° C., and the conversion rate is detected after 20 hours of reaction. After complete conversion, add n-hexane for extraction, then concentrate the extract, add 2.5mg VC as an antioxidant, and finally treat the mixed phospholipids with ethanol to obtain 44.5g powdered PS with a PS content of 62.86%.

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Abstract

The invention relates to a method of preparing compounds, in particular to a method of preparing powder phosphatidylserine. The invention has to overcome the problems that the prior art inputs a lot of equipment and costs a lot, and the transformation ratio of the reaction is hard to be guaranteed. The invention has the technical proposal that a method of preparing powder phosphatidylserine takes natural lecithin as the reaction substrate; and after being separated by water, the reaction substrate can be added with an inorganic system prepared by L-serine, calcium salt and buffer solution to produce rough product under the effect of phospholipase D. Concentrate can be obtained by conducting the solvent extraction to the rough product, and then can be purified by solvent to obtain phosphatidylserine. The invention is characterized in that to separate the reaction substrate needs water three to ten times (w / v) of reaction substrate; and the separating temperature is ranged from 20 DEG C to 60 DEG C. In the system, the weight of the L-serine is two to ten times of the reaction substrate, and the weight of water used in the inorganic system is three to ten times(w / v) of reaction substrate. Ph value of the inorganic system can be adjusted between 4.5 and 6.0 by the buffer solution.

Description

Technical field: [0001] The invention relates to a preparation method of a compound, in particular to a preparation method of powdered phosphatidylserine. Background technique: [0002] Phosphatidylserine (PS) is a member of the phospholipid family, and it is the only phospholipid that can regulate the functional state of key proteins in the cell membrane. Usually PS refers to a group of compounds, rather than a single component, because the acetyl residues of products from different raw materials vary greatly. PS exists in the biofilms of all animals, higher plants, and microorganisms. It is one of the important components of cell membrane phospholipids. It accounts for 10%-20% of all phospholipids in the mammalian brain and plays an important role in many cellular metabolic processes. Regulatory effect. Its main effect can improve the vitality of brain cells, and has a good effect on treating brain atrophy, preventing Alzheimer's disease, and improving the brain function...

Claims

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Application Information

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IPC IPC(8): C12P13/04
Inventor 宋晚平任静王磊李强董少为
Owner 杨凌萃健生物工程技术有限公司
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