Colour development protein chip using BCIP/NBT as substrate as well as application in detection of autoantibody thereof

Abstract

The invention discloses a color protein chip which substrate is BCIP / NBT. The invention is composed of a substrate, an array, 12 antigens coated on the array, negative contrast, positive contrast and blank contrast, wherein the substrate is a glass slide decorated by APES, the kinds and point sample densities of the 12 antigens are 520ug / ml ANA, 465ug / ml Ro-60, 530ug / ml La / SSb, 530ug / ml Jo-1, 525ug / ml Scl-70, 520ug / ml Sm, 615ug / ml Ro-52 / SSa, 340ug / ml RF, 465ug / ml CCP, 410ug / ml u1RNP, 490ug / ml CENP-B, 580ug / ml dsDNA. The invention uses the alkali phosphatase coloring method whose substrate is BCIP / NBT as the final check signal of protein chip to be widely used for self antibody check, while the check sensitivity has higher coincidence rate than prior art, with reduced check cost and time. The invention can be used in clinical check, sanitary supervision, regular sanitary supervision and scientific research or the like.

Description

(1) Technical field [0001] The invention relates to a protein chip and its application, especially an alkaline phosphatase chromogenic protein chip with BCIP / NBT as a substrate and its application in autoantibody detection; it belongs to technologies such as biochip and diagnostic reagents field. (2) Background technology [0002] Autoimmune diseases (AID) can involve multiple organs and systems, causing great harm to patients. In recent years, the incidence of AID has increased significantly, accounting for about 3% to 5% of the world's total population, and there are more than 120 million patients in my country. The appearance of autoantibodies is the most important feature of AID. The detection of autoantibodies not only plays an important role in the early diagnosis of AID, but also has important significance in many aspects such as disease typing, prognosis judgment and treatment effect monitoring. [0003] At present, the clinical autoantibody detection methods mainl...

AI Technical Summary

Problems solved by technology

However, the application of the NBT / BCIP colorimetric method in protein chips has not been reported at home and abroad so far. Therefore, it is necessary to combine this commonly used detection method with a new protein chip technology system to develop more suitable for different protein chips. A protein chip that is simple, fast, and simultaneously detects multiple antibodies or antigens required for level clinical testing

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