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Method for extracting flavone and polysaccharide from mulberry leaf

A technology for flavonoids and mulberry leaves, applied in the field of extracting flavonoids and polysaccharides, can solve the problems of low yield of active ingredients, low selectivity, long operation time, etc. Effect

Inactive Publication Date: 2008-07-09
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The decoction method has poor reproducibility, low selectivity, easy to destroy active ingredients, large solvent consumption, long operation time, low yield of active ingredients, and the fine powder of medicinal materials is easy to coagulate and coke, which is easy to cause waste of resources and is difficult to achieve mass production
The extraction method uses ethanol, acetone, water, etc. as solvents, which consumes a lot of solvents, takes too long to extract, and has a low yield of active ingredients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The mulberry leaves produced in Zhejiang were selected, air-dried, pulverized into powder with a pulverizer, and passed through a 60-mesh sieve.

[0026] Weigh 50g of mulberry leaf powder, add 1000ml of 70% ethanol according to the material-to-liquid ratio of 1:20, ultrasonically treat for 10min under 200W power; control the extraction temperature to 70°C, and extract for 2 hours.

[0027] Centrifuge the extract at 4000r / min for 10 minutes, take the supernatant and filter it to obtain the flavonoid filtrate of mulberry leaf. The content of flavonoids in mulberry leaf was determined to be 2.88% by spectrophotometry, and the yield of flavonoids was calculated to be 28.8 mg / g.

[0028] The flavonoid filtrate was concentrated under reduced pressure to obtain the flavonoid extract of mulberry leaves.

Embodiment 2

[0030] The mulberry leaves produced in Zhejiang were selected, air-dried, pulverized into powder with a pulverizer, and passed through a 40-mesh sieve.

[0031] Weigh 50g of mulberry leaf powder, add 2000ml of 80% ethanol according to the material-to-liquid ratio of 1:40, ultrasonically treat for 20min under 300W power; control the extraction temperature to 80°C, and extract for 1 hour.

[0032] Centrifuge the extract at 4000r / min for 10 minutes, take the supernatant and filter it to obtain the flavonoid filtrate of mulberry leaf. The content of flavonoids in mulberry leaves was determined to be 2.90% by spectrophotometry, and the yield of flavonoids was calculated to be 29mg / g.

[0033] The flavonoid filtrate is dried in an oven at 50° C. to 90° C. to obtain the flavone extract.

Embodiment 3

[0035] The mulberry leaves produced in Zhejiang were selected, air-dried, pulverized into powder with a pulverizer, and passed through a 60-mesh sieve.

[0036] Weigh 50g of mulberry leaf powder, add distilled water according to the ratio of solid to liquid 1:40, ultrasonically treat for 10min at 300W power; control the extraction temperature at 80°C, and extract for 1 hour.

[0037] Centrifuge the extract at 4000r / min for 10 minutes, take the supernatant and filter to obtain the mulberry leaf polysaccharide filtrate;

[0038] Add high-concentration ethanol to the polysaccharide filtrate to make the ethanol concentration in the final solution reach 70%, place it at 4°C for 18 hours, take the precipitate and dry it, and the yield of mulberry leaf polysaccharide is 122.5mg / g.

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Abstract

The present invention discloses a method of extracting flavone and polyoses from mulberry leaves, including air drying, grinding and screening. Mulberry leaf powder is immersed in 60 to 80 percent ethanol so as to extract flavone or in water so as to extract polyoses; the ultrasonic treatment with 100 to 400W power lasts for 5 to 25 minutes; extraction temperature is controlled within 60 to 85 DEG C and the extraction lasts for 1 to 2 hours; the extracting solution is centrifugalized and filtered by supernatant fluid, thus obtaining the filtrate; the flavone filtrate is concentrated so as to obtain the mulberry leaf flavone extract; or ethanol is added into the polyoses filtrate so as to make the ethanol concentration up to 70 percent in the final solution; the filtrate is placed for 18 to 24 hours under 4 to 10 DEG C, then deposited and dried, thus obtaining the mulberry leaf polyoses. The flavone yield can reach more than 25mg / g and the polyoses yield 110mg / g. The present invention, with the advantages of good repeatability, high selection, short operating time, less solvent consumption, high yield of effective ingredients, no noise, no pollution and relatively good application prospective, is applicable to industrial production.

Description

technical field [0001] The invention relates to a method for extracting flavonoids and polysaccharides from mulberry leaves. Background technique [0002] Mulberry leaves account for about 64% of the output of the aboveground part of mulberry trees, and can be picked 3 to 6 times a year, with strong vitality. Mulberry leaves are rich in bioactive components such as flavonoids and polysaccharides. As one of the main functional components of mulberry leaves, flavonoids account for about 1.0-3.0% of the dry leaf weight. They are the ones with the highest content in all plant stems and leaves. They have anti-oxidation, lower blood pressure, prevent arteriosclerosis, Anti-aging and other effects. The rich polysaccharides in mulberry leaves have significant effects of lowering blood sugar and inhibiting blood lipid elevation, and have multiple regulatory functions in the treatment of type II diabetes. [0003] At present, the extraction of flavonoids and polysaccharides from mu...

Claims

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Application Information

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IPC IPC(8): A61K36/605A61P3/06A61P9/10A61P39/06C07D311/30C08B37/00A61K127/00
Inventor 励建荣王芳应芝韩晓祥
Owner ZHEJIANG GONGSHANG UNIVERSITY
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