Application of alpha-hydroxy-acid in preparing cancer and tumor in vivo injection therapeutic medicine

A technology for treating drugs and hydroxy acids, which is applied in the field of medicine and can solve problems such as limitations in clinical applications

Inactive Publication Date: 2008-07-09
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because it is a radionuclide, the method must be kept up with medical protection measures, coupled with problems such as drug source supply, its clinical application is limited

Method used

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  • Application of alpha-hydroxy-acid in preparing cancer and tumor in vivo injection therapeutic medicine
  • Application of alpha-hydroxy-acid in preparing cancer and tumor in vivo injection therapeutic medicine
  • Application of alpha-hydroxy-acid in preparing cancer and tumor in vivo injection therapeutic medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Nude Mouse Experiment

[0029] Nude mouse human liver cancer tissue model (SMMC-LTNM, 255 generation, provided by Shanghai Jiading District Central Hospital) and 4-6 weeks old male BALB / C nude mice weighing 18-20 g were selected.

[0030]Cut the fresh non-necrotic tissue block of SMMC-LTNM's back subcutaneous transplantation tumor into a homogenate shape, suck a 0.2ml human syringe with a 16-gauge needle, and inject the tumor tissue under the skin of the back of another nude mouse to form a subcutaneous transplantation. After about 2-3 weeks, when it slowly absorbs and starts to grow, the tumor volume is measured at the specified time every day, and the longest diameter (A) and shortest diameter (B) of the tumor are measured with a vernier caliper. The tumor volume is V(mm 3 )=∏A×B 2 / 6, the tumor volume is 0.6±0.05cm as the selection criterion for tumor formation.

[0031] Three concentrations of glycolic acid of 15%, 20%, and 30% were selected respectively (the ...

Embodiment 2

[0035] Example 2 Rat experiment

[0036] After the rat was anesthetized with 10% chloral hydrate intraperitoneally, a small incision was made under the xiphoid process, and the rat liver can be seen after entering the abdominal cavity. Inject 50ul of 15% glycolic acid directly into the liver, and then close the abdomen. In addition, 50ul of 15% acetic acid was injected into mouse liver as a positive control group. The rats were sacrificed on the 4th day after the injection, and the diameter of the necrotic foci formed from the gross specimen was compared.

[0037] The results showed that glycolic acid can cause local coagulative necrosis of rat liver by injection, suggesting that it can be used for local injection to treat liver cancer. Moreover, it can be seen from the necrotic foci formed by acetic acid and glycolic acid on the liver of rats that glycolic acid has a similar effect to acetic acid.

Embodiment 3

[0038] Example 3 Inhibition of liver tumor cells in vitro

[0039] Cell culture: Liver tumor cell line 7721 (purchased from the cell bank of the Chinese Academy of Sciences) in DMEM medium (containing penicillin 100μg / ml and streptomycin 100μg / ml) containing 10% newborn calf serum at 37°C, 5% CO 2 Incubate in a cell incubator, and take cells in the logarithmic growth phase for experiments.

[0040] Inhibition of tumor cells in vitro: 7721 cells were trypsinized, blown away, and the cell concentration was adjusted to 5×10 4 / mL, added to 96-well culture plate, 100μL per well. Set at 37℃, 5% CO 2 , Cultivate for 24h under saturated humidity conditions. Remove the medium, add 120μL of fresh medium, and then add 30μL each of acetic acid and glycolic acid of different concentrations. Add the same amount of medium to the blank wells and control wells, and place them at 37 ℃, 5% CO 2 , Cultivate 48h under saturated humidity conditions. Add MTT at a concentration of 5mg / ml, 15μL / well, 37℃...

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Abstract

The present invention relates to the medicine technical field and concretely indicates an application of alpha-hydroxy acid for preparing a cancerous tumour internal injection treatment drug. The alpha-hydroxy acid of the present invention indicates hydroxyacetic acid or alpha-hydracrylic acid. With the present invention, the hydroxyacetic acid aqueous solution with different concentrations is prepared to be processed for medicine efficacy tests such as a naked rat experiment, a rat experiment and a cell test, which are compared with the medicine efficacy of electropositive medicine acetic acid used for liver cancer internal injection which has the same concentration with the hydroxyacetic acid aqueous solution. The naked rat experiment and a rat liver damnification experiment show that under the same concentration, the hydroxyacetic acid has similar anti-liver-cancer effect as and even better anti-liver-cancer effect than the acetic acid. The cell test shows that the hydroxyacetic acid is applied to the tumour internal injection with great inhibiting effect towards hepatic tumour, pulmonary tumour, oesophagus tumour, etc. The present invention also approves that the alpha-hydracrylic acid (lactic acid) has similar anticancer effect as the hydroxyacetic acid. The present invention provides the novel cancerous tumour internal injection treatment drug which has strong inhibiting effect towards tumour cells, such as the hepatic tumour, the pulmonary tumour, the oesophagus tumour, etc.

Description

Technical field [0001] The invention relates to the technical field of medicine, in particular to the application of alpha-hydroxy acid in the preparation of a therapeutic drug for injecting cancer tumors. Background technique [0002] Primary liver cancer (hepatocarcinoma for short) is one of the common malignant tumors with a high degree of malignancy, mainly hepatocellular carcinoma (HCC). Hepatectomy is still the best treatment method for liver cancer. However, due to the hidden incidence and rapid development of primary liver cancer, only about 10% of patients can be surgically removed at the time of diagnosis. Even if surgery is performed, there are still many cases that cannot be completely removed or tumors. It is difficult to avoid postoperative recurrence. For advanced liver cancer, surgery is usually impossible due to liver insufficiency. This part of patients needs to be treated by various non-surgical resection methods, or first non-surgical treatment to reduce the s...

Claims

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Application Information

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IPC IPC(8): A61K31/19A61P35/00
Inventor 陈建明李莹邓莉孙靖严俊胡育新吴忠斌张扬张悦郭丹
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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