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Immobilisation of antigenic carbohydrates to support detection of pathogenic micro-organisms.

An antigen, biosensor technology, used in chemistry and diagnostics, that can solve the problems of laborious, cumbersome, and unsuitable for large-scale screening programs

Inactive Publication Date: 2008-06-18
RNA HLDG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method is cumbersome, laborious and not suitable for large-scale screening programs by modern standards

Method used

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  • Immobilisation of antigenic carbohydrates to support detection of pathogenic micro-organisms.
  • Immobilisation of antigenic carbohydrates to support detection of pathogenic micro-organisms.
  • Immobilisation of antigenic carbohydrates to support detection of pathogenic micro-organisms.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0132] Materials and methods

[0133] 1.1 Materials

[0134] 1.1.1 Chemicals

[0135] Amine coupling kits were purchased from Biacore AB (Uppsala, Sweden), which also provided ready-to-use 10 mM glycine and 50 mM sodium hydroxide, the above-mentioned amine coupling kits included N-hydroxysuccinimide (NHS), 1- Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and ethanolamine hydrochloride-sodium hydroxide (pH 8.5) and running buffer (HBS-EP), the flow The buffer contained 10 mM HEPES, 150 mM sodium hydrochloride, 3 mM EDTA and 0.005% (v / v) surfactant P20 (pH 7.4). Ethanol, ethylene glycol, sodium chloride, sodium hydroxide and trichloroacetic acid (TCA) were purchased from Merck (Darmstadt, Germany). Carboxymethylated dextran sodium salt, sodium cyanoborohydride and carbohydrazide were obtained from Fluka Chemie GmbH (Buchs, Switzerland). CHAPS (Plus one) was supplied by Pharmacia Biotech (Uppsala, Sweden). Sodium acetate trihydrate and acetic acid were ...

Embodiment 2

[0271] introduction

[0272] SPR biosensor for detection of yolk antibodies reflecting Salmonella Enteritidis infection [AAB4]

[0273] Salmonella is one of the main causes of bacterial gastroenteritis in humans (Fischer, 2004; van Duynhoven et al., 2005). In the Netherlands, Salmonella Enteritidis serovar (S.e.) was the most frequently isolated serovar between 1994-1998 (Pelt et al., 1999). Within this serovar, eggs and egg products are the most important sources of infection. Despite several control measures, approximately 9% of the Dutch spawning hen flock is still infected each year. Because egg contamination with Salmonella remains a public health threat, it is important that hen flocks be detected for infection as quickly as possible through an appropriate surveillance program.

[0274] The current monitoring system in the Netherlands for grow-out hens during the laying period is based on serum profiles (Bokkers, 2002). The aim is to reduce the prevalence of S.e. and...

Embodiment 3

[0343] introduction

[0344] Direct detection of Campylobacter spp. using LPS-coated beads and monitoring phage infection.

[0345] In developed countries, Campylobacter is the most common foodborne pathogen causing gastroenteritis characterized by watery and / or bloody diarrhea. Campylobacter has been associated with Guillain-Barr syndrome (GBS), Reiter syndrome, and hemolytic uremic syndrome (HUS), as well as reactive arthritis (FSAI, 2002; Lake et al., 2003; Tauxe, 2000). In the last 20 years, the rate of Campylobacter infection has continued to increase in many developed countries, perhaps due to improvements in detection and reporting. In the United States, 2,400,000 cases of campylobacteriosis are reported annually, which corresponds to approximately 1% of the US population (Tauxe, 2000).

[0346] Wild birds and domestic animals are reservoirs of Campylobacter and shed the bacteria into the environment. Poultry are important vectors of Campylobacter infection in humans...

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Abstract

The invention relates to the field of chemistry and diagnosis, more in particular to diagnosis of current and / or past and / or symptomless infections or of a history of exposure to a gram-negative-bacterium (such as an enterobacteriaceae or a legionella). Even more in particular, the invention relates to the screening of animals or animal products for the presence of unwanted / undesired microorganisms. The invention further relates to a method for screening samples for the presence of antibodies directed against unwanted / undesired microorganisms and preferably such a method is performed with help of a biosensor. The invention also relates to a method for immobilising polysaccharides to solid surfaces. The invention furthermore provides solid surfaces with immobilised polysaccharides as well as applications of such surfaces.

Description

technical field [0001] The present invention relates to the fields of chemistry and diagnostics, more particularly to the diagnosis of current and / or past and / or asymptomatic infections or history of exposure to Gram-negative bacteria such as Enterobacter or Legionella. Even more specifically, the present invention relates to screening animals or animal products for the presence of unwanted / undesirable microorganisms. The invention further relates to a method for screening a sample for the presence of antibodies against unwanted / undesirable microorganisms and preferably implements such a method by means of a biosensor. The invention also relates to methods for immobilizing polysaccharides to solid surfaces. The invention further provides solid surfaces with immobilized polysaccharides and uses of such surfaces. Background technique [0002] The world is full of Gram-negative bacteria, many of which are members of the Enterobacteriaceae family. Members of this family are f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543
CPCG01N2400/10G01N33/54353G01N33/56911G01N2469/20G01N33/5308
Inventor 阿尔德特·安东尼·贝格韦夫格尔特鲁达·科尔内利娅·安东尼娅·玛丽亚·博克肯贝尔塔·杰拉尔达·玛丽亚·格特马克
Owner RNA HLDG
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