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Astaxanthin high-production mutant strain of haematococcus pluvialis

A technology of Haematococcus pluvialis and astaxanthin, applied in the direction of mutant preparation, single-cell algae, etc., can solve the problems of easy contamination by other organisms and slow growth during the cultivation process

Active Publication Date: 2008-05-07
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

[0003] Although Haematococcus pluvialis is currently the best source of astaxanthin for industrial production, it grows relatively slowly and is easily contaminated by other organisms during the cultivation process. In order to optimize the industrial production process and increase the production of astaxanthin, it is necessary to produce natural Astaxanthin provides superior algal strains to increase industrial yields

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[0014] One, the growth rate determination of Haematococcus pluvialis (Haematococcus pluvialis) E1 CCTCC M 207164 strain of the present invention:

[0015] (1) The medium is KM1 medium, formula: 1.2g / l sodium acetate, 2.0g / l yeast extract, 0.4g / l aspartic acid, 0.2g / l magnesium chloride hexahydrate, 0.01g / l heptahydrate Ferrous sulfate, 0.02g / l calcium chloride dihydrate, pH 6.8

[0016] (2) The algae are inoculated into a 250ml Erlenmeyer flask containing 100ml of sterile KM1 medium at an inoculum size of 10%, and in a 12h light-dark cycle, the temperature is 22°C, and the light intensity is 20 μmolm -2 the s -1 Cultured in a light incubator, shaking the flask three times a day.

[0017] (3) Take a sample every 12 hours, count the cells with a hemocytometer, and draw a growth curve according to the cell number (average value) of 3 parallel samples, with the formula μ=(LnNt-LnNo) / (t-t 0 ) to calculate the growth rate (μ is the specific growth rate, Nt is the cell value at ti...

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Abstract

The present invention provides a high-yielding astaxanthin mutant strain of Haematococcus pluvialis (Haematococcus pluvialis) E1 CCTCC M 207164, which uses the wild strain of Haematococcus pluvialis as material , using ultraviolet chemical compound mutagenesis, mutagenesis selection of carotenoid biosynthesis inhibitor screening. The astaxanthin content of the algal strain is 1.83 times higher than that of the original strain, the growth rate is 14% higher than that of the original strain, the physiological and biochemical characteristics are stable, and it has stable genetic properties. It is an excellent algal strain for producing natural astaxanthin and can be used as a source of industrial production. Industrial production of natural astaxanthin.

Description

technical field [0001] The invention relates to a high-yield astaxanthin mutant strain of Haematococcus pluvialis selected for producing natural astaxanthin. Background technique [0002] Astaxanthin is a dark brown-red carbonylated carotenoid, its chemical name is 3,3'-dihydroxy-β,β'-carotene-4,4'-dione, it not only has Unique coloring function, also exhibits super strong antioxidant properties and a variety of biological activities, known as the strongest natural antioxidant vitamin in nature, its antioxidant capacity is 550 times that of vitamin E, more than other carotenoids It has broad application prospects in the fields of aquatic products and poultry farming, food, cosmetics, medicine, etc. For decades, the production of natural astaxanthin by various methods has been a hot issue that has been widely concerned at home and abroad. At present, the best source of natural astaxanthin for industrial production is Haematococcus pluvialis, which is a freshwater unicellular...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N15/01
Inventor 彭娟向文洲何慧陈峰
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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