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Micro-fluidic chip and its preparation and uses

A microfluidic chip and chip technology, applied in chemical instruments and methods, material testing products, solid adsorbent liquid separation, etc., can solve the problem of limited polymerization reaction system, difficult positioning of the monolithic column for thermally induced polymerization, and organic solvent resistance. Problems such as poor sex, to achieve the effect of a simple production method

Inactive Publication Date: 2008-04-30
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method has developed rapidly, due to the limited polymerization reaction system and the poor tolerance of organic polymer monolithic columns to organic solvents, and the positioning of thermally induced polymerized monolithic columns in microchannels is difficult, it is difficult to become A General Chip SPE Method

Method used

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  • Micro-fluidic chip and its preparation and uses
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  • Micro-fluidic chip and its preparation and uses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Such as figure 1 As shown, a microfluidic chip is formed by sealing the upper and lower two-layer substrates. The upper substrate is provided with a sample inlet 1, a sample outlet 2 and a microchannel. The microchannel of the upper substrate A microparticle introduction channel and its inlet 3 are provided on the top, and a dam structure is respectively provided between the inlet 3 of the microparticle introduction channel, the sample inlet 1 and the sample outlet 2, and the two dam structures form a double cofferdam structure 4, two The microchannel between the two dam structures forms the packed column bed channel 5; the position corresponding to the dam structure on the lower substrate is respectively provided with a microchannel, and there is a gap between the top surface of the dam structure and the microchannel of the lower substrate; The depth of the slot is smaller than the depth of the microchannels in the upper substrate.

[0026] Fabrication of microfluidic...

Embodiment 2

[0034] Example 2 The chip SPE column filled with unmodified silica gel (diameter 5 μm) is used for the extraction of λ-DNA

[0035] The loading, washing and elution buffers used in this example were 6M guanidine hydrochloride (dissolved in 10mM Tris-1mM EDTA buffer, pH 6.1), 80% isopropanol in water and TE buffer (10mM Tris-1mM EDTA , pH 7.5); the sample used for extraction was λ-DNA dissolved in loading buffer (final concentration was 0.5 ng / μL). The self-made chip interface was used to connect the SPE chip to the syringe pump, and the flow rate was controlled at 3 μL / min; the volumes of the sample loading, washing and eluting solutions used were 12 μL, 15 μL and 15 μL, respectively, and the entire chip extraction process took less than 15 minutes. Eluted DNA samples were collected and quantified fluorophotometrically using intercalating fluorescent dyes to determine the extraction efficiency and reproducibility of this chip SPE method.

[0036] From the experimental results...

Embodiment 3

[0037] Example 3 The chip SPE column filled with silica gel is used to purify genomic DNA from actual samples

[0038] The sample used in this example is a human whole blood sample, and the processing method is as follows: 4.6 μL whole blood sample is dissolved in 500 μL loading buffer (6M guanidine hydrochloride, 10 mM Tris-1 mM EDTA buffer, pH 6.1), and then 10 μL proteinase K is added (20mg / mL), and then kept in a 56°C water bath for 10 minutes to obtain a sample solution for extraction. The volumes of loading, washing and elution solutions used were 15 μL, 20 μL and 15 μL, respectively, and the flow rate was 3 μL / min. The eluted DNA samples were collected in 5 μL aliquots, and 2.5 μL in each aliquot was used for PCR amplification. The primer sequences used are:

[0039] 5'-AACTGTTGCTTTATAGGATTTT-3' (sense strand),

[0040] 5'-AGGAGCTTATTGATAACTCAGAC-3' (antisense strand);

[0041] The amplified product is a 650bp fragment of the human genome β-globin gene. The composit...

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Abstract

The invention relates to a microfluidic chip, in particular to a microfluidic chip and its preparation and application as a solid phase extraction column. The sample inlet, the sample outlet and the microchannel are provided with a particle introduction channel and its inlet on the microchannel of the upper substrate, and a dam structure is respectively provided between the inlet of the particle introduction channel and the sample inlet and the sample outlet, The two dam structures form a double cofferdam structure, and the microchannel between the two dam structures forms a packed column bed channel; microchannels are respectively arranged on the lower substrate corresponding to the dam structure, and the top surface of the dam structure and the lower layer There are gaps between the micro-channels of the substrate; the depth of the gaps is smaller than the depth of the micro-channels in the upper substrate. The main advantages of the present invention are: simple and fast production, good repeatability, wide application range and good application effect.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a microfluidic chip and its preparation and application as a solid phase extraction column. Background technique [0002] Solid phase extraction (SPE, or liquid-solid extraction) refers to the process of purifying or concentrating trace components in liquid samples by using the separation principle of selective adsorption and selective desorption. Solid phase extraction (SPE) technology is widely used in separation / purification, concentration or enrichment of trace analytes, removal of interfering substances (such as salts, etc.), and improvement of concentration and detection sensitivity of analytes. Approach. The miniaturization of SPE technology, that is, microfluidic chip SPE technology, refers to the use of microchannel structures formed by chip micromachining technology and control units such as microvalve / micropump to complete SPE operations on a microfluidic chip platform. Miniatu...

Claims

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Application Information

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IPC IPC(8): G01N33/48B01D15/00
Inventor 林炳承钟润涛戴忠鹏
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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