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Nitric oxide-blocked cross-linked tetrameric hemoglobin

A technology of hemoglobin and nitric oxide, applied in hemoglobin/myoglobin, peptide/protein components, blood diseases, etc., can solve problems such as slow infusion rate

Inactive Publication Date: 2008-04-30
AGCO GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in critical trauma patients who urgently require large volumes of blood, it is not possible to use slower infusion rates

Method used

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  • Nitric oxide-blocked cross-linked tetrameric hemoglobin
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  • Nitric oxide-blocked cross-linked tetrameric hemoglobin

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0090] II. Preparation method of amidomethylated cross-linked hemoglobin

[0091] figure 1 The steps of some embodiments of the invention are described. These steps may be performed independently of each other or one after the other, which is not a limitation of the invention. The method of the present invention may omit one or more steps. The preparation method of hemoglobin of the present invention may include: step 101, which includes removing plasma proteins and endotoxins from products containing red blood cells by washing; step 102, which includes lysing these red blood cells; step 103, which includes removing endotoxins from the lysed red blood cells Matrix (comprising membrane and white blood cell) to separate hemoglobin; Step 104, comprises removing the oxygen in hemoglobin; Step 105, comprises adding the reagent that can provide sulfhydryl protecting group for cysteine ​​of hemoglobin in hemoglobin solution; Step 106, comprises Isolating hemoglobin with a sulfhy...

Embodiment 1

[0238] Comparing two methods of primary processing of whole blood

[0239] Material: Bovine Blood Collection: The bovine blood was collected into a 1 gallon container that could hold 100 ml of a 6% sodium ethylenediaminetetraacetate (EDTA) solution and cooled on ice.

[0240] Whole bovine arterial blood was divided into batches A and B. Batch A consisted of 2200 ml whole blood, washed with a haemonetics cell saver (Cell Saver) 5 to obtain platelet-free, coagulation factor-free, extracellular potassium-free, anticoagulant-free and cell-matrix-free concentrated red blood cells (Method A). Batch B consisted of 1800 ml whole blood washed with Millipore 0.65 μm filter (Method B).

[0241] Method A. Removal of plasma proteins with a cell harvester 5: Red blood cells were concentrated from other fractions of freshly collected anticoagulated bovine blood using a Haemonetics cell harvester 5 . Do not burst white or red blood cells at this time.

[0242] After passing through a...

Embodiment 2

[0260] lysed white blood cells

[0261] This example shows that the relative time to WBC lysis was determined. Preferential lysis of RBCs compared to WBCs optimizes erythrocyte lysis for maximum hemoglobin and does not introduce proteases to lysed WBCs.

[0262] Method: 2000ml of whole blood was only filtered through the 100μ reservoir filter of the cell harvester 5 . Then 200ml of blood was injected into 7 beakers. One beaker was designated as control. Then specify specific lysis times for the remaining 6 beakers: 30 s, 1 min, 2 min, 3 min, 4 min, 5 min. Add 910ml of 0.9% saline solution to the control beaker and start timing. At times increasing to 30 seconds, 1, 2, 3, 4 and 5 minutes, 10 ml samples were taken for white blood cell analysis.

[0263] For the remaining 6 beakers, add 800ml DI water. After 30 seconds, 110 ml of 9% saline was added to the first beaker to stop lysis. After stirring for about 30 seconds, a 10 ml sample was taken for leukocyte analysis. A...

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Abstract

The present invention includes compositions containing carboxamidomethylated cross-linked hemoglobin where the cysteine moiety of the hemoglobin includes a thiol protecting group and where the hemoglobin has a reduced ability to bind with nitric oxide. Preferably, the hemoglobin is deoxygenated, endotoxin free, and stroma free. The present invention also includes method of preparation, process of preparation and the method of use including supplementing blood volume in mammals and treating disorders in mammals where oxygen delivery agents are of benefit.

Description

[0001] This application claims priority to US Provisional Application Serial No. 60 / 853,968, filed October 23, 2006, with first author Ross Tye. technical field [0002] The present invention relates to nitric oxide blocked cross-linked tetrameric hemoglobin, more particularly carboxamidomethylated cross-linked tetrameric hemoglobin, which has low reactivity with nitric oxide (NO), Cross-linking stabilizes the tetramer for in vivo use. It also discloses its preparation method and its application as a blood volume expansion agent and an oxygen transport therapeutic agent. Background technique [0003] One of the limitations of using blood in emergencies is the need for blood typing and cross-matching to minimize the risk of transfusion reactions. Blood typing and cross matching takes at least 10 minutes, and complete blood typing and cross matching can take up to an hour. In addition, there is an estimated risk of HIV transmission in 1 in 500,000 blood samples and an estima...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/805A61K38/42A61P7/06
Inventor R·W·泰
Owner AGCO GMBH
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