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Mushroom fermentation oral liquid with anti-tumor activity and preparation method

A technology of anti-tumor activity and oral liquid, which is applied in the field of preparation of products after fermentation of advanced fungi

Inactive Publication Date: 2012-06-13
DALIAN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the anti-tumor preparation of the fermented liquid of Lentinus edodes

Method used

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  • Mushroom fermentation oral liquid with anti-tumor activity and preparation method
  • Mushroom fermentation oral liquid with anti-tumor activity and preparation method
  • Mushroom fermentation oral liquid with anti-tumor activity and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the preparation of mushroom fermented liquid

[0019] (1) Preparation of Lentinus edodes Fermentation Medium

[0020] glucose

1g

fresh brewer's yeast

8g

sucrose

6g

whole milk powder

1g

Peptone

1g

Potassium dihydrogen phosphate

0.3g

Vitamin B1

0.01g

Vitamin B2

0.01g

[0021] Add the above items into 1000ml of distilled water, mix them and put them in a water bath at 50°C to dissolve them completely, adjust the pH to 7.5, divide them into 300ml Erlenmeyer flasks, each bottle is 250ml, and cover with 4 layers of gauze and a layer of kraft paper to seal. , sterilized for 15 minutes, cooled for later use.

[0022] (2) Take the mycelium of shiitake mushroom C91-3 that grew well on the 7th day, and inoculate it into the above-mentioned medium, and inoculate 1 g of mycelium in each bottle.

[0023] (3) Culture on a shaker at room temperature, at 75 rpm for the first two days, a...

Embodiment 2

[0025] Embodiment 2: the preparation of mushroom fermented oral liquid

[0026] (1) Mix the above-mentioned shiitake mushroom yeast liquid with distilled water in a ratio of 1:5, add 4% xylitol at the same time to make an oral liquid, use the existing technology to divide into bottles, disinfect and sterilize it and put it in 4 ℃ refrigerator.

[0027] (2) Mix the above-mentioned shiitake mushroom yeast liquid with distilled water according to the ratio of 1:6, add 6% xylitol at the same time to make oral liquid, utilize the existing technology to divide into bottles, disinfect and sterilize and mix in 4 ℃ refrigerator.

[0028] (3) Mix the above-mentioned shiitake mushroom yeast liquid with distilled water according to the ratio of 1:7, add 8% xylitol at the same time to make oral liquid, utilize the existing technology to divide into bottles, disinfect and sterilize and put in 4 ℃ refrigerator.

Embodiment 3

[0029] Embodiment 3: In vitro antitumor activity test of fermented mushroom preparation

[0030] (1) Selection of cell lines: H22 (mouse ascites type liver cancer cell line), U14 (mouse ascites type cervical cancer cell line), S1 80 (mouse ascites type sarcoma cell line).

[0031] (2) MTT method to measure the anti-tumor rate of mushroom fermented preparation in vitro: choose cells in the logarithmic growth phase of various tumor cells and adjust the concentration to 1×10 5 per ml, and inoculated in 96-well culture plate, 100 μl per well. In the control group, 100 μl of sterile saline was added to each well, and in the experimental group, 100 μl of mushroom fermented oral liquid was added. Set up 3 replicate wells for each group and place at 37°C, 5% CO 2 After culturing in the incubator for 24, 48 and 72 hours, add 100 μl of MTT (1 μg / ml), continue to cultivate for 4 hours, centrifuge to discard the supernatant, then add 100ul DMSO to each well, shake and mix well to fully ...

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Abstract

Mushroom oral liquid with antineoplastic activity comprises a culture medium which is made from glucose, fresh brewers' yeast, sucrose, whole milk powder; peptone, monobasic potassium phosphate, vitamin B1 and B2 charged into distilled water. The culture medium inoculates mycelium of mushroom C91-3 through submerged fermentation, centrifuging under 4 DEG C, being filtered again and fermentation mother liquid is obtained by charging edulcorator. The experiment proves that the ratio of inhibiting tumor of H22 (little mouse ascites liver cancer cell strain), U14 (little mouse ascites uterine neck cell strain) and S180 (little mouse ascites caruncle cell strain) to the fermentation of the medicament after seventy-two hours is separately 71.11%, 72.85% and 76.81% in the external and antineoplastic test with stronger and direct action of killing tumour cell. The tumor bearing mice cure rate of H22 is 35% and the tumor bearing mice cure rate of S180 is 40% in the internal and antineoplastic test. Even though survival phase of dead mice is longer than control group, and the action of controlling tumor is obvious.

Description

Technical field: [0001] The invention belongs to the field of antineoplastic drugs, and relates to a preparation derived from the fermented product of higher fungi. Background technique: [0002] Fungi have been used as a source of medicine for many years, and medicines produced from fungi have been used clinically in my country for a long time. Lentinus edodes is one of the world's rare edible and medicinal fungi, with high nutritional and medicinal value. Lentinus edodes has complex chemical components, strong pharmacological activity, and a wide range of clinical applications. Its anti-virus, anti-tumor, immune function regulation, and stimulation of interferon formation have attracted widespread attention from scholars at home and abroad (Jing Jun, et al. Chinese Journal of Food Hygiene. 2001: 13(2):46-47; Chihara G, et al. Cancer Res. 1970:30:2776). Modern medical research believes that polysaccharides, as biological response modifiers (BRM), play a major role in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/06A61P35/00C12N1/14
Inventor 黄敏宁安红钟民涛曹婧
Owner DALIAN MEDICAL UNIVERSITY
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