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Mushroom fermentation dry powder preparation with anti-tumor activity and preparation method

A technology of anti-tumor activity and dry powder preparation, which is applied in the field of preparation of products after fermentation of advanced fungi

Inactive Publication Date: 2012-06-13
DALIAN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the anti-tumor preparation of the fermented liquid of Lentinus edodes

Method used

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  • Mushroom fermentation dry powder preparation with anti-tumor activity and preparation method
  • Mushroom fermentation dry powder preparation with anti-tumor activity and preparation method
  • Mushroom fermentation dry powder preparation with anti-tumor activity and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the preparation of mushroom fermented liquid

[0023] (1) Preparation of Lentinus edodes Fermentation Medium

[0024]

[0025] Add the above items into 1000ml of distilled water, mix them and put them in a water bath at 50°C to dissolve them completely, adjust the pH to 7.5, divide them into 300ml Erlenmeyer flasks, each bottle is 250ml, and cover with 4 layers of gauze and a layer of kraft paper to seal. , sterilized for 15 minutes, cooled for later use.

[0026] (2) Take the mycelium of shiitake mushroom C91-3 that grew well on the 7th day, and inoculate it into the above-mentioned medium, and inoculate 1 g of mycelium in each bottle.

[0027] (3) Culture on a shaker at room temperature, at 75 rpm for the first two days, and gradually increase the speed to 100 rpm on the third day. Observe the growth every day, aseptically take a small amount of culture to observe the number of spores, if the number of spores reaches 10 7 / ml, the culture was stopp...

Embodiment 2

[0029] Embodiment 2: Preparation of the freeze-dried raw powder of mushroom fermented liquid

[0030] (1) Concentrate and freeze-dry the supernatant in a vacuum freeze dryer to obtain the freeze-dried raw powder of Lentinus edodes C91-3 fermentation broth, and store it in a -20°C refrigerator.

Embodiment 3

[0031] Embodiment 3: the preparation of mushroom fermented dry powder capsule

[0032] Weigh 1 mg of the freeze-dried powder, and pack the freeze-dried powder into capsules using the existing technology.

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Abstract

A mushroom fermentation dried powder medicament with antineoplastic activity takes mushroom C91-3 bacterial strain as raw material, which is alutaceous or golden ocher solid powder through submerged cultuse, filtering and freezing, and the pH value of water solution is 5.0-8.0. Instant granule, troche, capsule, oral liquid and the like can be made from the dried powder and adjunct. The experiment proves that the ratio of inhibiting tumor of H22 (little mouse ascites liver cancer cell strain), U14 (little mouse ascites uterine neck cell strain) and S180 (little mouse ascites caruncle cell strain) to the fermentation of the medicament after seventy-two hours is separately 71.11%, 72.85% and 76.81% in the external and antineoplastic test. The invention has the action of curing the caruncle in the internal and antineoplastic test. The tumor bearing mice cure rate of H22 is 35%, the tumor bearing mice cure rate of S180 is 40% with obvious action of inhibition tumor. Animal toxic experiment proves that the invention has safety and reliability without the toxic.

Description

Technical field: [0001] The invention belongs to the field of antineoplastic drugs, and relates to a preparation derived from the fermented product of higher fungi. [0002] Background technique: [0003] Fungi have been used as a source of medicine for many years, and medicines produced from fungi have been used clinically in my country for a long time. Lentinus edodes is one of the world's rare edible and medicinal fungi, with high nutritional and medicinal value. Lentinus edodes has complex chemical components, strong pharmacological activity, and a wide range of clinical applications. Its anti-virus, anti-tumor, immune function regulation, and stimulation of interferon formation have attracted widespread attention from scholars at home and abroad (Jing Jun, et al. Chinese Journal of Food Hygiene. 2001: 13(2):46-47; Chihara G. et al. Cancer Res. 1970:30:2776). Modern medical research believes that polysaccharides, as biological response modifiers (BRM), play a major rol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/06A61K9/14A61P35/00C12N1/14
Inventor 黄敏钟民涛宁安红曹婧
Owner DALIAN MEDICAL UNIVERSITY
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