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Application of p53 expression product in preparing medicine for treating tumor

A p53 gene and tumor technology, applied in the field of biomedicine, can solve the problems of limited application, lack of tumor cell specificity, toxic side effects, insensitivity to chemotherapy and radiotherapy, etc.

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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, chemotherapy and radiotherapy are still the first choice for the treatment of cancer. Although the two have achieved considerable curative effect on the treatment of tumors, they have relatively large toxic and side effects due to the lack of specificity to tumor cells, and some tumor cells have a certain effect on chemotherapy. and insensitivity to radiotherapy, thus limiting their clinical application to a large extent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1p53

[0027] Example 1 p53 induces apoptosis of human lung cancer cells (H1299)

[0028] Acquisition of the p53 gene: the full-length cDNA fragment of KILLER was amplified from the human kidney cDNA expression library by PCR, and the length was 1182bp; it was cloned into the eukaryotic expression vector after confirmation by sequencing.

[0029] Human lung cancer cells (H1299) were provided by ATCC. The cell line uses GIBCO company RPMI1640 complete medium to culture H1299 human lung cancer cells. The tumor cells were transfected with the plasmid expressing p53 for 48 hours, and the cells were collected for PI staining, and the cell apoptosis was detected by flow cytometry.

[0030]The result shows that the p53 of the present invention can induce the apoptosis of human lung cancer cells (H1299).

Embodiment 2

[0031] Example 2 p53 induces apoptosis of human colon cancer cell HCT116

[0032] Acquisition of the p53 gene: the full-length cDNA fragment of KILLER was amplified from the human kidney cDNA expression library by PCR, and the length was 1182bp; it was cloned into the eukaryotic expression vector after confirmation by sequencing.

[0033] Human colon cancer cell HCT116 was provided by ATCC. The cell line was cultured with Macoy's 5A complete medium (containing 10% calf serum, 100 U / ml penicillin and 100 U / ml streptomycin) of GIBCO Company. The tumor cells were transfected with the plasmid expressing p53 for 48 hours, and the cells were collected for PI staining, and the cell apoptosis was detected by flow cytometry.

[0034] The results show that the p53 of the present invention can promote the increase of the number of apoptotic cells in human colon cancer (HCT116).

Embodiment 3

[0035] Example 3 Attenuated Salmonella typhimurium expressing p53 can induce tumor cell apoptosis

[0036] The tumor cells were cultured in complete medium without resistance, and the cells were infected with 250 MOI (the ratio of the number of virus particles to the number of tested cells) expressing p53 and negative control attenuated Salmonella typhimurium for 3 hours, and then treated with antibiotics and 100 μg / ml G418 complete medium to grow cells. The cells were infected at different time points, and the induction of tumor cell apoptosis by attenuated Salmonella typhimurium expressing p53 was detected.

[0037] The results showed that the attenuated Salmonella typhimurium expressing p53 could induce tumor cell apoptosis.

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Abstract

The invention belongs to the biomedical field and relates to the application of p53 in preparing for a drug preparation for remedying the tumor. A p53 gene coding product is a transcription regulator which can induce the apoptosis or the block of the cell cycle by regulating lower target gene. An over-expression of the p53 can very effectively induce the apoptosis of tumor cells. Attenuated salmonella typhimurium can be used as a carrier to introduce the p53 to express inside the tumor cells and kill the tumor cells. The development of a novel treatment drug which considers the salmonella with the p53 as orally taken gene not only solves the problem of non-injection path of a protein polypeptide drug but also more importantly provides the tumor treatment with a safe, effective, peculiar and economical method.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of p53 expression products in the preparation of medicaments for treating tumors. Background technique [0002] Tumor disease has now risen to be the No. 2 "killer" in the world, and its death toll is second only to cardiovascular disease. Over the past few years, domestic and foreign medical circles have gained a new understanding of the pathogenesis of tumor diseases on the basis of cells. Based on the further understanding of the pathogenesis of tumors, people use various approaches to research and develop drugs that can specifically and effectively kill tumor cells without toxicity to normal cells. At present, chemotherapy and radiotherapy are still the first choice for the treatment of cancer. Although the two have achieved considerable curative effect on the treatment of tumors, they have relatively large toxic and side effects due to the lack of specificity to tumo...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K38/17A61P35/00C12N15/74
CPCY02A50/30
Inventor 王树林任大明符薇朱向莹
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