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Goat growth trait GH gene clone and its application as molecule mark

A technology of growth traits and genes, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as the relationship between unanalyzed polymorphisms and growth traits, birth weight, etc.

Inactive Publication Date: 2008-03-05
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Malveiro found a polymorphism site in exon 5 of goat GH gene related to milk production; Li Meiyu et al. The Hae III restriction polymorphism was found in the incubators, but the relationship between the above polymorphisms and growth traits was not analyzed; Min Lingjiang et al. showed that the birth weight and year-old weight of GH gene AA individuals were significantly higher than those of BB and AB types (P< 0.05)

Method used

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  • Goat growth trait GH gene clone and its application as molecule mark
  • Goat growth trait GH gene clone and its application as molecule mark

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1: Application of molecular markers prepared by the present invention in association analysis of growth traits of Boer goats

[0062] The breed of the test flock was Boer goat commercial flock, and 194 DNA samples were used for genotype detection. The growth traits for association analysis included: birth weight, birth length, birth height, birth bust, weaning weight, weaning body length, weaning body height, weaning bust, lactation daily gain and 11-month-old body weight.

[0063] The genotype detection results of HaeIII-RFLP polymorphism site of goat GH gene showed that there were 30 individuals with AA genotype and 164 individuals with AB genotype in 194 individuals. The results of the least square mean and standard deviation analysis of traits between the AA and AB genotypes are shown in Table 1. The results of the correlation analysis showed that the growth traits of the AB genotype goats were better than those of the AA genotypes, and the birth bust of the...

Embodiment 2

[0067] Example 2: Application of the molecular markers of the present invention in association analysis of goat growth traits II

[0068] Association analysis of HaeIII-RFLP polymorphism at A43G site of GH gene and some production traits in Boer goat population,

[0069] The sample used and the character of carrying out statistical analysis are the same as example 1.

[0070] The genotype test results show that the CC genotype accounts for the vast majority of the 194 individuals, and 161 individuals have the CD genotype, and 33 individuals have the CD genotype. Table 2. Analysis results show that, except for birth length, other traits of CC genotype individuals are higher than those of CD genotype individuals, and there is a correlation between this site and goat growth traits.

[0071] Table 2: Least square mean analysis of partial production traits of individuals with different GH genotypes

[0072] character

Embodiment 3

[0073] Example 3: Application of the molecular markers of the present invention in association analysis of goat growth traits three

[0074] In the Boer goat population, the association analysis was carried out on the combination genotypes of GH gene A55G and A43G loci and some production traits. The samples used and the traits for statistical analysis were the same as Example 1.

[0075] The results of genotype detection showed that there were 4 combined genotypes in 194 individuals, including 18 AACC types, 143 ABCC types, 12 AACD types, and 21 ABCD types. The least square mean and The results of the standard deviation analysis are shown in Table 3. The analysis results show that: the growth traits of individuals with ABCD genotype are significantly higher than those of other genotypes, and the growth traits of individuals with AACD genotype are lower than those of other genotypes. The genotype effects of different combinations are as follows: : ABCD>AACC>ABCC>AACD.

[0076...

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Abstract

The present invention belongs to the field of animal gene engineering, and is especially the cloning and polymorphism application of goat's growth hormone (GH) gene. The present invention features that two DNA sequences of goat's GH gene as shown is obtained through cloning and two base mutations affecting goat's growth characters are detected. The present invention discloses two goat's GH gene sequences and two base mutational sites affecting goat's growth characters. The present invention also discloses the preparation process and application of the said genes, and provides new molecular markers for the auxiliary selection of goat's growth characters.

Description

technical field [0001] The invention belongs to the technical field of animal genetic engineering, and in particular relates to the cloning of goat growth trait genes and its application in marker-assisted selection. Background technique [0002] Growth traits are one of the most important economic traits in animal husbandry production. The breeding work in the past few decades has made remarkable achievements. However, conventional breeding methods are often limited to the phenotypic traits of animals, and the process of genetic improvement is slow slow. In recent years, with the development of molecular biology technology, animal breeding has gradually penetrated into the molecular field. Scholars at home and abroad have conducted a lot of research on the relationship between molecular genetic markers and production performance. Appropriate genetic markers are of great benefit to carrying out marker-assisted selection, implementing early selection, improving the accuracy ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12Q1/68
Inventor 杨利国滑国华吴灿杰李乔乐张春艳于俊娜张颖
Owner HUAZHONG AGRI UNIV
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