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CBA reagent kit for detecting EB virus gp78 antibody and method for making same

A technology of Epstein-Barr virus and kit, applied in the field of medicine and biology, to achieve high sensitivity, eliminate subjectivity, and reduce errors

Active Publication Date: 2008-01-30
SUN YAT SEN UNIV CANCER CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology has the advantages of fast measurement speed, easy operation, good stability, and high sensitivity. It has been used in routine clinical examinations of many diseases. However, at present, there is still no successful detection of anti-EB in serum by flow cytometry. Viral membrane antigen gp78 antibody kit

Method used

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  • CBA reagent kit for detecting EB virus gp78 antibody and method for making same

Examples

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Embodiment Construction

[0023] The Epstein-Barr virus gp78 antigen used in this example is a chemically synthesized polypeptide. The amino acid sequence of this polypeptide is an antigenic determinant obtained by computer software analysis. It has strong immunogenicity, hydrophobicity and high surface exposure probability. During the synthesis process Six carbon atoms and biotin are coupled to the amino terminus. The polypeptide sequence is as follows: gp78(BILF2): TK-19, Biotin-Ahx-TSTSHRPHRRPVSKRPTHK, (AHX=6 carbons).

[0024] The synthetic peptides listed in the series table were synthesized by Shenzhen Hanyu Bioengineering Co., Ltd.

[0025] Goat anti human IgG, Fc γ specific R-PE (goat anti-human IgG, Fc γ Segment, R-PE marker) was purchased from JacksonImmunoResearch;

[0026] R-Phycoerythrin-AffiniPure Goat Anti-Human Serum IgA, alpha Chain Specific (goat anti-human IgA, alpha chain, R-PE label) was purchased from Jackson ImmunoResearch;

[0027] Elution buffer: 1×PBS, 0.05% Tween-20; Stor...

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Abstract

The invention discloses a CBA kit used for detecting anti-EB virus gp78 antibody, which includes coupling superbead, the superbead is made by coupling blank superbead and NeutrAvidin in a coupling way, the NeutrAvidin binds with the bioepiderm labeled EB virus gp78 antigen which is coupled with Biotin. The gp78 antibody CBA kit is suitable for crown screening, evaluation of the high incidence of nasopharyngeal carcinoma, diagnose and prognosis effect appraisal, observation, furthermore, the kit can be applied directly in nasopharyngeal carcinoma early diagnosis, judge prognosis of patients, etc. with the characteristics of rapid, objectivity and accurate.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to a CBA kit for detecting serum Epstein-Barr virus gp78 antibody for flow cytometry and a preparation method thereof. Background technique [0002] Epstein-Barr virus (EBV) belongs to the Gammaherpesviridae family, and its basic structure includes three parts: nucleus, capsid and envelope. The capsid is icosahedral and consists of 162 shell particles. The envelope is composed of the nuclear membrane of the infected cell, on which there is a membrane glycoprotein encoded by the virus, which has the functions of recognizing the Epstein-Barr virus receptor on the lymphocyte and fusion with the cell. In addition, there is a protein envelope between the capsule and the capsid. Epstein-Barr virus can only proliferate in B lymphocytes, which can transform them and pass them down for a long time. Cells infected by the virus have the genome of EBV and can produce...

Claims

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Application Information

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IPC IPC(8): G01N33/571G01N33/53
Inventor 曾益新谷爱娣谢彦博
Owner SUN YAT SEN UNIV CANCER CENT
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