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Rat-rabbit family thin element protein and its cDNA sequence

A leptin protein, DNA molecule technology, applied in the field of leptin protein

Inactive Publication Date: 2008-01-30
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, the material basis for the development and utilization of most leptin products at home and abroad are obtained from the isolation of animals in ordinary environments. Sensitive genes for resistance to environmental stress, as a characteristic animal, the scientific research on the ecological adaptation mechanism of this extreme environment, as well as the development and utilization of its products are blank at home and abroad

Method used

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  • Rat-rabbit family thin element protein and its cDNA sequence
  • Rat-rabbit family thin element protein and its cDNA sequence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. The nucleotide sequence and amino acid sequence of the leptin cDNA containing the complete coding region of the Inner Mongolia grassland Ochotona daurica bedfordi pika

[0032] During the implementation of the invention, the total RNA of the white adipose tissue in the Ochotona daurica bedfordi pika in the Inner Mongolia grassland was extracted, and the specific primers PKLEPFOR and PKLEPREV for amplifying the leptin cDNA fragment containing the complete coding region of the pika family were designed (see SEQ ID NO: 3 and SEQ ID NO: 4). The cDNA fragment was amplified by RT-PCR method, and after the target fragment was recovered and purified by electrophoresis gel, it was connected into the PMD-18T cloning vector (Dalian Bao Biological Co., Ltd.), transformed into DH5α E. coli competent bacteria, and after overnight culture, picked White recombinant colonies, after enrichment culture, extract plasmid DNA and identify insert fragments after PstI and EcoR I res...

Embodiment 2

[0034] Example 2. The leptincDNA nucleotide sequence and amino acid sequence containing the complete coding region of the Ochotona nubrica pika on the Qinghai-Tibet Plateau

[0035] During the practice of the present invention, the total RNA of the white adipose tissue in the Ochotona nubrica pika on the Qinghai-Tibet Plateau is extracted, and the amplification primers and cloning method are the same as those shown in Example 1.

[0036] The present invention clones a leptin cDNA sequence containing a complete coding region, a total of 646 bp, named NubrLep gene. The sequence analysis method is the same as in Invention 1. The gene reading frame is located between 11-514bp, 504bp in total, the start codon is ATG, and the stop codon is TGA. The deduced protein sequence shows that the pika leptin protein consists of 167 amino acids, and contains a signal peptide sequence consisting of 21 amino acids at its N-terminus. The mature protein consists of 146 amino acids with a predic...

Embodiment 3

[0037] Example 3. The leptincDNA nucleotide sequence and amino acid sequence containing the complete coding region of Ochotona cansus pika on the Qinghai-Tibet Plateau

[0038] During the implementation of the present invention, the total RNA of the white adipose tissue in the Ochotona cansus pika on the Qinghai-Tibet Plateau is extracted, and the amplification primers and cloning methods are the same as those shown in Example 1.

[0039] The present invention clones a leptin cDNA sequence containing a complete coding region, a total of 646 bp, named CansLep gene. The sequence analysis method is the same as in Invention 1. The gene reading frame is located between 11-514bp, 504bp in total, the start codon is ATG, and the stop codon is TGA. The deduced protein sequence shows that the pika leptin protein consists of 167 amino acids, and contains a signal peptide sequence consisting of 21 amino acids at its N-terminus. The mature protein consists of 146 amino acids with a predi...

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Abstract

The invention provides a new leptin protein and the DNA sequence of the code thereof. The new leptin protein comes from cold-adaption animals-mice and rabbit families in extreme cold environment pressure. The invention lays the foundation for the protection, development and utilization of the leptin proteins of mice and rabbit family materials with Chinese characteristics and for the application in biological engineering products of mice and rabbit families.

Description

technical field [0001] The present invention relates to leptin (leptin) protein, especially the leptin protein of the cold-adapted animal pika family under extreme cold environment pressure and the cDNA sequence containing the complete coding region sequence. Background technique [0002] my country is rich in biological resources, and the effective protection and utilization of its unique resources has become a powerful driving force for my country's social and economic development. The unique animal and plant resources on the Qinghai-Tibet Plateau in my country are unique in the world. The extreme pressure environment of high cold, low oxygen, and strong ultraviolet radiation not only brings challenges to the survival of animals and plants here, but also endows these characteristic animals and plants with new functions or increases in original functions during the long-term adaptive evolution process To meet the needs of survival in such a stressful environment. Advantag...

Claims

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Application Information

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IPC IPC(8): C07K14/575C12N15/12
Inventor 杨洁
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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