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Carbon nano tube modified blood sugar biosensor

A biosensor and carbon nanotube technology, applied in the field of biosensor development, can solve problems such as low applicability and unstable measurement results, and achieve the effects of improving response sensitivity, accelerating electron transfer rate, and improving electron transfer efficiency

Inactive Publication Date: 2008-01-09
ZHEJIANG UNIV +1
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AI Technical Summary

Problems solved by technology

This technical solution uses glucose oxidase as a catalyst, and its accuracy is affected by the atmospheric oxygen pressure and concentration at the measurement site and the oxygen content in the blood sample. The measurement results are unstable and the applicability is not high.

Method used

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  • Carbon nano tube modified blood sugar biosensor
  • Carbon nano tube modified blood sugar biosensor

Examples

Experimental program
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Effect test

Embodiment

[0035] Silver paste was coated on polypropylene substrate by screen printing technique to form silver / silver chloride pseudo-reference electrode; then, conductive carbon paste was printed on substrate to form working and counter electrodes; insulating layer was printed , keep the exposed area of ​​the working electrode, the counter electrode and the pseudo-reference electrode constant.

[0036] Use 4.8 mg / ml sodium dodecylbenzene sulfonate and 0.5% Triton X-100 by volume to prepare a 2.5 mg / ml suspension of multi-walled carbon nanotubes, vibrate in ultrasonic for 2 hours, and form a stable suspension. Add 3 microliters of the carbon nanotube suspension dropwise into the reaction chamber of each electrode with a pipette, place in a drying oven and dry in the dark for 24 hours to prepare an electrode system modified with carbon nanotubes.

[0037] Then, 2 microliters of glucose dehydrogenase containing 4780 U / ml of pyrroloquinoline quinone as a coenzyme, 0.1 M of potassium ferr...

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Abstract

The invention provides a blood sugar biosensor of carbon nanometer tube, it includes floor, three electrode system, insulation, forming the reaction lumen body around three electrode system, setting the conversion zone in the reaction lumen body, laying over the cover plate on the reaction lumen body, the conversion zone includes, enzyme and electron transport agent, the enzyme is the glc dh pyrrole and quinone for coenzyme. The transducer's corresponding sensitivity improves evidently, it has not infection for measuring the dextrose deepness in the blood with the oxygen pressure, thickness in the measuring site and oxygen content of blood sample. The invention has the excellence of easy manufacture artwork, low cost, suited to volume-produce and the low sample quantity etc.

Description

technical field [0001] The invention belongs to the field of biosensor development, and specifically refers to a glucose dehydrogenase blood glucose biosensor modified with carbon nanotubes and using pyrroloquinoline quinone as a coenzyme and a preparation method thereof. Background technique [0002] As our country enters the information society, the population structure tends to be aging, people pay more and more attention to health, people's living standards improve year by year, and the prevalence of diabetes also increases accordingly. The total prevalence rate in 13 regions of China is 0.67 %, about 1% in big cities, and the prevalence rate increases significantly after the age of 40. The accuracy of the glucose sensor using glucose oxidase as a catalyst is affected by the atmospheric oxygen pressure and concentration at the measurement site and the oxygen content in the blood sample. [0003] In 1991, Professor Iijima of NEC in Japan developed a coaxial tubular nanos...

Claims

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Application Information

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IPC IPC(8): G01N27/403G01N27/327G01N27/26A61B5/1486
Inventor 李光徐惠王酉
Owner ZHEJIANG UNIV
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