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High ORAC value oligomeric proanthocyanidin and purifying method for the same

A technology of oligomeric proanthocyanidins and proanthocyanidins, which is applied in the direction of pharmaceutical formulations, plant raw materials, medical preparations containing active ingredients, etc., can solve the problems of low yield of oligomeric proanthocyanidins, small sample loading, and low membrane flux, etc., and achieve Increased membrane flux and sample loading, shortened ultrafiltration time, and reduced energy consumption

Inactive Publication Date: 2008-01-09
连平劲创生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The purpose of the present invention is aimed at above-mentioned existing method and the membrane flux that the product obtained according to these methods exists is little, required membrane area is big, loading sample amount is little, ultrafiltration time is long and it is difficult to realize large-scale industrial production, oligomeric proanthocyanidin content Not high, low yield of oligomeric proanthocyanidins and other deficiencies and defects, provide a high ORAC value oligomeric proanthocyanidins and purification method to obtain the product

Method used

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  • High ORAC value oligomeric proanthocyanidin and purifying method for the same
  • High ORAC value oligomeric proanthocyanidin and purifying method for the same
  • High ORAC value oligomeric proanthocyanidin and purifying method for the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] 1. The purification of proanthocyanidins rough purification product

[0056] 1. After crushing the grape seeds, use 55% ethanol to carry out dynamic circulation extraction in a multifunctional ethanol extraction tank to obtain an extraction concentrate;

[0057] 2. Extract the concentrated solution and add 6 times the volume of water, stir and mix well, then sink in water for 16 hours;

[0058] 3. Filtrate and separate the above-mentioned water sinking liquid to obtain a clear filtrate;

[0059] 4. Adsorb the filtrate to the HPD400A macroporous adsorption resin column, so that the active ingredients in the filtrate are adsorbed by the resin. The loading was 0.625 grams of filtrate of the feedstock per gram of dry resin.

[0060] 5. Wash the resin with 3-4 column volumes of deionized water at a flow rate of 3-4 column volumes / hour;

[0061] 6. Using 50% ethanol to desorb the active ingredient adsorbed by the resin to obtain a desorption liquid. The flow rate of the e...

Embodiment 2

[0070] 1. The purification of proanthocyanidins rough purification product

[0071] Same as Example 1

[0072] 2. Purification of oligomeric proanthocyanidins with high ORAC value by membrane ultrafiltration

[0073] 1. Weigh 10kg of the crudely purified proanthocyanidin product above, fully stir and dissolve it with 200L 70°C reverse osmosis water, pass the solution through a 100-mesh sieve, and add it to the feed liquid tank of the organic membrane flat-panel ultrafiltration device;

[0074] 2 Use a flat organic membrane with a relative molecular weight cut-off of 1 million (membrane model JK S-172) for continuous ultrafiltration. During ultrafiltration, the temperature of the material is controlled at about 40°C, and the return valve is adjusted to control the pressure of the feed liquid inlet to 8.0bar. , the outlet pressure was 7.0 bar, and the filtrate was collected, concentrated by reverse osmosis, and spray-dried to obtain 2520 grams of oligomeric proanthocyanidins wi...

Embodiment 3

[0077] 1. The purification of proanthocyanidins rough purification product

[0078] 1. After crushing the pine bark, use 55% ethanol to carry out dynamic circulation extraction in a multifunctional ethanol extraction tank to obtain an extraction concentrate;

[0079] 2. Extract the concentrated solution and add 6 times the volume of water, stir and mix well, then sink in water for 16 hours;

[0080] 3. Filtrate and separate the above-mentioned water sinking liquid to obtain a clear filtrate;

[0081] 4. Adsorb the filtrate to the HPD400A macroporous adsorption resin column, so that the active ingredients in the filtrate are adsorbed by the resin. 0.625 grams of filtrate of starting material per gram of dry resin.

[0082] 5. Wash the resin with 3-4 column volumes of deionized water at a flow rate of 3-4 column volumes / hour;

[0083] 6. Using 50% ethanol to desorb the active ingredient adsorbed by the resin to obtain a desorption solution. The flow rate of the ethanol solut...

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Abstract

An oligo-proanthocyanidin with high ORAC value and its purification are disclosed. The products contain oligo-proanthocyanidin 45-65wt%, ORAC value is 15000 - 19000 mu-mol TE / g. The process is carried out by dissolving for proanthocyanidin crude product in solvent proportionally, adding into organic feed barrel of organic film ultra-filtration device, ultra-filtering for feed by organic film with molecular weight between 50000-1000000, concentrating and drying to obtain final product. It's convenient and efficient, has more film throughput and yield, and its costs low.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, in particular to extracting and separating oligomeric proanthocyanidins with high ORAC value from grape seeds. Background technique [0002] Proanthocyanidins are composed of monomers such as catechin ((+)-catechin), epicatechin ((-)-epicatechin) and epicatechin gallate ((-)-epicatechin-3-gallate) through C4→ Polyphenols formed by condensation of C8 or C4→C6 bonds. Because such substances can produce colored substances anthocyanidins (anthocyanidins) when heated in an acidic medium, they are named proanthocyanidins. [0003] [0004] According to different degrees of polymerization, proanthocyanidins are further divided into oligomeric proathocyanidins (OPC) and polymeric proathocyanidins (PPC). Proanthocyanidins with a degree of polymerization above 7 are called high-polymeric proanthocyanidins, and proanthocyanidins with a degree of polymerization of 2-7 are called low-p...

Claims

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Application Information

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IPC IPC(8): C07D311/04A61K36/756A61K131/00
Inventor 张孝友冯建光
Owner 连平劲创生物技术有限公司
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