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Microorganism preparation for modifying water body by using bacterial and manufacturing method thereof

A technology for improving microbial preparations and strains, which can be applied to microorganism-based methods, biochemical equipment and methods, microorganisms, etc., and can solve the problems of many complex strains, disadvantageous promotion and application, and narrow application scope.

Inactive Publication Date: 2007-11-28
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nitrifying bacteria in this technology are only suitable for low-temperature aquaculture environments such as seawater aquaculture, and the application range is narrow. At the same time, they need to cooperate with corresponding bioreactors. There are many compound strains and cumbersome steps, which is not conducive to the promotion and application in production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Step 1: Breeding of production strains

[0047] a. Collection of strain source: Collect a little bottom sludge and 300ml of waste water at the kitchen waste water outlet, add two drops of Gliese reagent dropwise, observe whether the solution is red or pink, and judge the nitrous acid content according to the color depth . Add 2 drops of diphenylamine reagent dropwise, observe whether the color is blue, and judge the nitric acid content according to the depth of the color;

[0048] b. Enrichment culture: Select the above-mentioned waste water with darker color as the screening source, add it to the enrichment medium of sterilized nitrifying bacteria and nitrosifying bacteria at a ratio of 2% respectively, and shake it at 200r / min in an environment of 30°C Bed culture was cultivated for 7 days, and the generation of nitrite was tested on the white porcelain plate with Gliese reagent every 2 days.

[0049] After 7 days, the culture solution turns red, indicating the pres...

Embodiment 2

[0074] Step 1: Breeding of production strains

[0075] c. Separation of strains: Before separation, the enrichment solution was passed into carbon dioxide for 0.5 hours, and then inoculated with the enrichment culture solution on the flat plate and silica gel plate prepared by washing agaric as a coagulant, and cultured at 30°C for 7 days, and observed The obtained pinpoint-sized colony is the purified bacterial species, which is preserved in the test tube of the separation medium.

[0076] e. Production and seed production: transfer the strains of the enriched liquid of nitrifying bacteria into the eggplant bottle equipped with the isolation medium for nitrifying bacteria, and transfer the strains of the enriched liquid of nitrosobacteria into the bottle of the enriched liquid of nitrosobacteria. In the eggplant bottle, transfer the Bacillus licheniformis species into the eggplant bottle of the nutrient agar medium, and cultivate it at 35°C for 45 hours. The surface of the eg...

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Abstract

The invention relates to a Microbial Product which can decompose the organic pollutant in farming waters rapidly and digestion the content of ammonia nitrogen and nitrite in water in time with using nitrobacteria, Bacillus endospores and other bacteria. The characteristic of this product was that the percent by weight before compound was Nitroso-bacteria 20%-40%, nitrifier 20-40%, Bacillus licheniformis 20%-60%. This invention using screening environments and adding concentration technology, at the same time combine directional concentration with fermentation to finish the process of enrich and enlarge the nitrifier by one step . This can reduce the culture time of nitrifier obviously, so that the using of nitrifier would from laboratory to industrialization. The ten times amount about Nitrobacteria than liquid product can complement and assort with high concentration Bacillus endospores to maintain the water with the best farming condition of rich, live, fecund and clear. This product can basically replaced the chemical medicine using in breed aquatics and produce the organic farm produce with green, safe and no antibiotic residue.

Description

technical field [0001] The invention relates to a microbial preparation and a preparation method for improving water bodies by using strains. By directly adding them to water bodies, nitrifying bacteria can be used to quickly degrade the content of ammonia nitrogen and nitrous acid in water bodies. Pollutants are decomposed into small molecular substances, which are utilized and absorbed by beneficial algae and other plankton in the water body to form a benign ecological environment suitable for the growth of aquatic animals, which belongs to the technical field of agricultural biological products. Background technique [0002] With the vigorous development of aquaculture intensification in our country, the pollution of aquaculture water environment is becoming more and more serious. The variety of microorganisms has increased and the speed of transmission has accelerated, and the occurrence of biological diseases in aquaculture has become more and more serious, causing seri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/10
Inventor 江瀚
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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