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Globular bacillus and its use

A technology of Bacillus sphaericus and bacterial strains, which is applied in the restoration of bacteria and contaminated soil, water/sludge/sewage treatment, etc., can solve the problem of insufficient xylene selectivity, save processing time, improve processing efficiency, Enhance the effect of biological treatment technology

Active Publication Date: 2007-11-21
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ES2085239 discloses a strain of Pseudomonas (CECT 4501) and its method for the biodegradation of aromatic hydrocarbons. The deficiency of this microorganism is that its selectivity to xylene is not good enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1, isolation and cultivation of Bacillus sphaericus B.sphaericus 2J1

[0015] 1. Isolation of strains

[0016] Sludge samples were collected at the sewage treatment plant of Fushun Petroleum No. 3 Plant. Take 1 drop of sludge aqueous solution on the screening medium, add 0.1g of xylene, spread it evenly with a spreader, put it in an incubator, and cultivate it at 30°C for 2 days; then use an inoculation loop to transfer the screened strains to LB The culture medium is placed in an incubator at 30° C. for 1 day, and the obtained strain is the strain that degrades xylene.

[0017] The composition of described screening medium is:

[0018] (NH 4 ) 2 SO 4 5g; K 2 HPO 4 1g;

[0019] K H 2 PO 4 4g; NaCl 2g;

[0020] MgSO 4 ·7H 2 O 1g; Agar 15g;

[0021] Water 1000mL; pH 7.0.

[0022] 2. Culture of strains

[0023] Use an inoculation loop to inoculate the strains screened in shake flasks with a liquid volume of 50 mL, and culture them ...

Embodiment 2

[0031] The cultivation of B. sphaericus 2J1 strain was the same as in Example 1. Take 2 shake flasks, add 50mL sterile water and 1mL toluene respectively. One of the shake flasks was taken as a blank control group, and the cultured 2J1 bacterial agent was added to the other shake flask to make its OD value 4.2. The two shaking flasks were placed on a shaker to shake the reaction, and the conditions of the shaker were: temperature 30° C.; rotation speed 150 rpm. Sampling and analysis after 10 hours, the degradation results of xylene are shown in Table 2.

[0032] initial

Embodiment 3

[0034] The cultivation of B. sphaericus 2J1 strain was the same as in Example 1. Take 2 shake flasks, fill them with 50mL sterile water, and then add 4g of soil contaminated by 1mL xylene. Take one shake flask as a blank control group; add the cultured B.sphaericus 2J1 bacterial agent to the other shake flask to make its OD value 5.0. Place 2 shake flasks on a shaker to shake the reaction. Shaking table conditions are: temperature 30°C; rotation speed 150 rpm. After 10 hours, samples were taken for analysis, and the results are listed in Table 3.

[0035] initial

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PUM

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Abstract

The Staphylococcus xylous 2J1 is prepared by separating out from active sludge in sewage disposal tank of petrochemical plant, purifying and culturing. The preservative number is CGMCC No. 1486. It has excellent efficient degradable performance for dimethyl benzene and can be used to dispose waste water and waste gas containing dimethyl benzene, polluted water and soil.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of bacillus sphaericus and its application. Background technique [0002] Spills and leaks of petroleum and various fuel oils, as well as leakage of underground oil storage facilities have caused groundwater to be polluted by aromatic hydrocarbons, which has become very serious worldwide. Among them, monocyclic aromatic hydrocarbon pollutants such as benzene, toluene, and xylene have attracted the most attention. They are the most water-soluble compounds in petroleum and various fuel oils, and constitute the most common organic pollutants in groundwater. Moreover, they belong to volatile organic compounds, which have become a large class of air pollutants second only to particle pollutants, which seriously pollute the atmosphere and are extremely harmful. As an important chemical raw material, organic synthesis, rubber synthesis, fiber synthesis, paint, dye, pet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B01D53/84B09C1/10C12R1/07
CPCY02A50/20
Inventor 范峰佟明友黎元生许谦
Owner CHINA PETROLEUM & CHEM CORP
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