Tobacco virus abrasive paper frictional inoculation method
A tobacco virus and sandpaper technology, applied in the directions of viruses/phages, biochemical equipment and methods, microorganisms, etc., can solve the problems of difficult operation, cumbersome procedures, large error in the amount of inoculated virus, etc., and achieves obvious effects, simple operation, and material selection. easy effect
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Embodiment 2004~20
[0019] EXAMPLES From 2004 to 2006, the field drug efficacy test of tobacco common mosaic disease was carried out in Dafang County, Guizhou Province every year, and there were about 3,500 flue-cured tobacco plants in more than 3 mu of land; from 2004 to 2006, every year in Jinsha County, Guizhou Province The China Tobacco Variety Breeding Base screens and identifies mosaic varieties. The test materials are about 5 mu of land and about 5,000 to 6,000 flue-cured tobacco plants.
[0020] 1. Take 20 g of leaves (leaf veins removed) of typical tobacco common mosaic disease symptoms, add a small amount of water and grind in a mortar, filter with gauze and squeeze out the juice, then use 1 to 5 L of distilled water to make up the volume to obtain the inoculum;
[0021] 2. Take 2 pieces of commercially available sandpaper, divide them into 8 small pieces of equal area, and fold them in half to form edges;
[0022] 3. Dip the inoculum with the edge of the sandpaper and gently draw it fr...
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