Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Soluble maize protein, its preparing method and use

A technology of zein and alkali solubility, which is applied in the preparation method of peptides, chemical instruments and methods, cellulose/protein conjugated artificial filaments, etc., can solve the problems of poor protein film strength, poor temperature resistance, and high brittleness. Achieve good temperature resistance, improve flexibility, high oxygen barrier effect

Inactive Publication Date: 2007-08-29
北京赛特瑞科技发展有限公司
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the traditional zein film is alcohol-soluble, the cost is high, and the protein film has poor strength, high brittleness, and poor temperature resistance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take by weighing 333.3 g of corn gluten powder, wherein protein is calculated by 60% in the corn gluten powder. Soak corn gluten powder in 1000ml of water at 50°C for 3 hours, take it out, rinse with cold water until the liquid is clear, squeeze dry, and the solid with liquid is 45%. Add 15% NaOH aqueous solution at a ratio of 3:1 to adjust the pH to 11. Stir for 4 hours at 60°C with a rotation speed of 700r / min. Centrifuge at 3000r / min for 10min at 20°C to obtain the supernatant. Add 0.05 g / l cysteine ​​to the supernatant, keep warm at 50° C., stir for 2 hours, and rotate at 400 r / min. Centrifuge at 4000r / min; 10min, 20°C, take the supernatant. Add 1.7g / l glutaraldehyde, keep warm at 40°C, stir for 2 hours, and rotate at 400r / min. Add ammonium sulfate, cooperate with zone electrophoresis, the zone width is 4cm, the voltage gradient: 12v / cm, the electrophoresis time: 30min, and the temperature is 40°C to precipitate the protein. Use glucose gel G-25 to desalt the c...

Embodiment 2

[0027] Take by weighing 333.3 g of corn gluten powder, wherein protein is calculated by 60% in the corn gluten powder. Soak the corn gluten powder in 50°C, 1000m water for 2 hours, take it out and rinse it with cold water until the liquid is clear, squeeze dry, and the solid with liquid is 55%. Add 15% NaOH aqueous solution at a ratio of 3:1 to adjust the pH to 11. Stir for 4 hours at 80°C with a rotation speed of 700r / min. Centrifuge at 3000r / min for 10min at 20°C to obtain the supernatant. Add 0.05 g / l cysteine ​​to the supernatant, keep warm at 50° C., stir for 2 hours, and rotate at 400 r / min. Centrifuge at 6000r / min; 10min, 20°C, take the supernatant. Add 1.7g / l glutaraldehyde, keep warm at 40°C, stir for 2 hours, and rotate at 400r / min. Add magnesium sulfate, cooperate with zone electrophoresis, the zone width is 4cm, voltage gradient: 12v / cm, electrophoresis time: 30min, temperature 40°C, to precipitate protein. Use glucose gel G-25 to desalt the column. After dry...

Embodiment 3

[0029] Take by weighing 333.3 g of corn gluten powder, wherein protein is calculated by 60% in the corn gluten powder. Soak the corn gluten powder in 1000ml of water at 50°C for 3 hours, take it out and rinse it with cold water until the liquid is clear, squeeze it dry, and the solid with liquid is 40%. Add 15% NaOH aqueous solution according to the liquid-to-solid ratio of 5:1, and adjust the pH to 14. Stir for 3 hours at 60°C with a rotation speed of 700r / min. Centrifuge at 3000r / min for 10min at 20°C to obtain the supernatant. Add 0.1 g / l cysteine ​​to the supernatant, keep warm at 50° C., stir for 2 hours, and rotate at 400 r / min. Centrifuge at 6000r / min; 10min, 20°C, take the supernatant. Add glutaraldehyde 1.7g / l, keep warm at 40°C, stir for 2 hours, and rotate at 400r / min. Add magnesium sulfate, cooperate with zone electrophoresis, the zone width is 4cm, voltage gradient: 12v / cm, electrophoresis time: 30min, temperature 40°C, to precipitate protein. Use glucose gel...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a caustic solubility zein, manufacture method and the application. The zein molecule chain has aldehyde group, and the molecular weight is 100-50000. The raw material is convenient to gain, and the producing technology is easy. The distilled residue could be used as forage. It has high social benefits and social benefits.

Description

1. Technical field [0001] The present invention relates to a kind of zein and its preparation method and application, more particularly to an alkali-soluble zein and its preparation method and application. 2. Background technology [0002] At present, there is no alkali-soluble zein in China, and the production technology abroad is mainly based on the production of alcohol-soluble technology. The general process is to use corn gluten powder as raw material, extract with monoalcohol or polyalcohol, such as ethanol or isopropanol, and then use freezing or cold water to precipitate the protein. Because the solvent is alcohol in the process, on the one hand, the amount of solvent used is very large, which increases the production cost; on the other hand, the application field of the obtained protein is not wide, and there are certain limitations. [0003] Serious environmental pollution caused by the increasing consumption of non-renewable resources and the non-degradability of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K2/00C07K1/26C07K1/16A61K8/64A61Q1/00D01F8/02C07K14/415
Inventor 陈洪章王开利任玉枝崔进
Owner 北京赛特瑞科技发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com