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A producing method of open-mouthed bait for aquaculture

A technology for opening bait and production method, which is applied in the molding or processing of animal feed, animal feed, animal feed, etc., can solve the problems of high cost and unstable nature, and achieves a short production cycle, is conducive to growth, and is not easy to pollute water quality. Effect

Inactive Publication Date: 2007-08-01
NANJING NORMAL UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Using fermented lower filamentous fungi to produce bacteria to make aquatic open bait, to solve the problem of high cost or unstable properties of existing bait

Method used

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  • A producing method of open-mouthed bait for aquaculture

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Experimental program
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Effect test

Embodiment 1

[0021] The preparation process is shown in Figure 1

[0022] (1) Activation of strains and preparation of seed solution: Take out Saprolegniaceae ATCC36051 from the refrigerator at 4°C, transfer to a PDA slope, grow at 25°C for 3 days, pour sterile water into it, scrape the hyphae, and transfer to human PDA liquid medium, 25°C, 140r / min fermentation culture for 2 days.

[0023] (2) Fermentation tank fermentation mycelium: use GBCS-5 type automatic fermentation tank; 5L tank is filled with 3L fermentation broth, inoculum size: 20%, ventilation rate: 1.5-3VV / M, 200rpm speed, 22 ° C, pH natural During the fermentation process, sterile water was added on the 2nd, 3rd, and 4th day respectively. On the 4th day of fermentation, the temperature was lowered to 15°C, and the aeration was increased to 3 at the same time, and the fermentation time was 6 days. It can be enlarged step by step to 1 ton or 5 tons according to needs.

[0024] (3) Collection of mycelium: the mycelia were fil...

Embodiment 2

[0027] Activate Pythium ultimum (Pythium ultimum) ATCC32231 slant, prepare seed solution, insert 20% inoculum into fermenter culture medium, ferment for 6 days at 22°C, 200rpm, collect mycelium by filtration, add carotene, amino acid, freeze-dry , by using adhesives or water-insoluble substances such as alginate, gelatin, nylon-protein, gliadin, lipids, cholesterol, etc. to mix various nutrients to form film or capsule-like microparticles, which are used for the opening of prawns bait.

Embodiment 3

[0029]Activate the slope of Mortierella alpine (Mortierella alpine) ATCC32222, prepare the seed liquid, put it into the medium of the fermenter according to the inoculation amount of 20%, ferment for 6 days at 22°C and 200rpm, collect the mycelium by filtration, add vitamins and amino acids, and dry Or boiling granulation, the compound feed is emulsified, and a terminating agent is added to make the colloid aggregate to form various particles, and the particles are solidified to form microcapsules, which are used as bait for eels.

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Abstract

The invention relates to a method for producing bait for fish. The method comprises: filtrating and collecting filamentous fungus used for producing polyunsaturated fatty acid after fermentation, adding compound nutritional ingredients, stirring uniformly, and making into microparticle or microcapsule baits with routine method. The inventive method has the advantages of no restriction of the environment and season, short production cycle, low cost, simple production process, no addition of fat, stable property, and convenience for storage and carrying. The mycelium after lyophilizing has better absorbability and adherence to nutritional ingredients, and is in favor of water purification.

Description

technical field [0001] The invention relates to the field of aquatic bait, in particular to a method for producing aquatic bait by using filamentous fungi producing polyunsaturated fatty acids. Background technique [0002] Since the 1970s, researchers at home and abroad have carried out a series of researches on the development and application of open baits for aquatic species, trying to develop artificially combined open baits for the production of rare aquatic products such as shrimp, crabs, shellfish, and seawater fish. To replace the natural biological baits such as unicellular algae, rotifers, cladocerans and artemia that the aquatic animal breeding production relies on, to overcome the shortcomings of natural baits such as high cost, great influence by seasons and weather, serious pollution, and unstable quality ( Jones et al., 1974; Kanazawa et al., 1982; Bautista et al., 1989; D'Abramo, 2002). For a long time, my country's aquatic seed production has mainly adopted...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/16C12N1/14A23K10/18A23K40/10A23K40/25A23K50/80
Inventor 戴传超赵沫杨启银
Owner NANJING NORMAL UNIVERSITY
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