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Production of gamma-propalanine and its special reactive column

A technology of aminobutyric acid and reaction column, which is applied in the direction of fermentation, etc., can solve the problems of individual microorganisms that cannot be reused, the cost of transformation increases, and the consumption of cultivation, etc., and achieve the effects of environmental protection, convenient post-processing, and simple materials

Inactive Publication Date: 2009-11-25
山东水晶生物科技股份有限公司
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AI Technical Summary

Problems solved by technology

In terms of microbial transformation, Lactobacillus, Escherichia coli and some Lactobacillus plantarum are commonly used as microbial strains for transformation, but microbial transformation requires additional consumption of culture media and other materials for maintaining microbial growth. In addition, individual microorganisms cannot be reused. Low is also a major factor in the increase in conversion costs

Method used

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  • Production of gamma-propalanine and its special reactive column
  • Production of gamma-propalanine and its special reactive column

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Embodiment 1

[0022] Embodiment 1, immobilized enzyme method reaction production gamma-aminobutyric acid

[0023] Escherichia coli CICC 10003: purchased from China Center of Industrial Culture Collection (CICC). In the agar slant preservation medium, store in a refrigerator at 4°C, and passage once every 3 months.

[0024] Preservation medium or activation medium (CICC29# medium): beef extract 1%, peptone 1%, sodium chloride 0.5%, glucose 1%, agar powder 1.5-2.0% (in solid medium), adjust pH 7.0 .

[0025] Enzyme production medium: peptone 1%, sodium chloride 1%, yeast extract 0.5%, sodium glutamate 0.5%, vitamin B6 (0.01-0.05mmol / L).

[0026] Immobilized enzyme regeneration solution: pH4.6, containing 0.05mol / L CaCl 2 , 0.01-0.05mM pyridoxal phosphate, 1-2.5mm / L sodium glutamate.

[0027] 1. Production of γ-aminobutyric acid

[0028] The production process is as follows:

[0029] Bacteria culture-ultrasonic crushing-enzyme immobilization-enzymolysis reaction-ion exchange-carbon parti...

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Abstract

The invention discloses a method for producing gamma-aminobutyric acid. The method is to crush the bacterium capable of expressing glutamic acid decarboxylase, immobilize it with sodium alginate to form immobilized glutamic acid decarboxylase, and make the immobilized glutamic acid decarboxylase and glutamic acid or glutamine The acid salt is subjected to an enzyme-catalyzed reaction in the reaction column to obtain γ-aminobutyric acid. The method of the invention utilizes biologically active enzymes, only needs to provide suitable buffer conditions, less substances in the conversion system, less by-products, no harmful substances, and is safe and reliable as raw materials for food and medicine. The entire technological process consumes less material and energy, generates less waste, and is conducive to environmental protection.

Description

technical field [0001] The invention relates to a method for producing gamma-aminobutyric acid and a special reaction column thereof. Background technique [0002] γ-aminobutyric acid (GABA for short) is a natural active ingredient. It is not a common amino acid that makes up proteins, so it is called a non-protein amino acid and is widely distributed in animals and plants. Current studies have proven that GABA is an inhibitory transmitter substance in the central nervous system. It is one of the most important neurotransmitters in brain tissue and an important inhibitory neurotransmitter that has been studied in depth. It acts as an inhibitory neurotransmitter in the system and participates in the physiological activities of cerebral circulation. In addition, γ-aminobutyric acid also has many functions such as lowering blood pressure, preventing arteriosclerosis, regulating arrhythmia, and preventing skin aging. Although GABA can be converted from glutamic acid in the bra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00
Inventor 孙君社乔春楠刘萍
Owner 山东水晶生物科技股份有限公司
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