Zinc-enriched Armillariella tabescens Polysaccharide and its preparation and use

A zinc-brightening bacteria, zinc-rich technology, applied in fungi, pharmaceutical formulations, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2009-06-10
ANHUI PROVINCE KANGMEILAI DABIESHAN BIOTECHCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solid-state fermentation and liquid submerged fermentation methods of Leucobacterium have been reported in China, but the use of solid-state fermentation and liquid submerged fermentation to enrich Leucobacteria zinc and prepare zinc-enriched Leucobacterium polysaccharides from it, and to further study its anti-radiation biological activity, has not been seen so far. to report

Method used

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  • Zinc-enriched Armillariella tabescens Polysaccharide and its preparation and use
  • Zinc-enriched Armillariella tabescens Polysaccharide and its preparation and use
  • Zinc-enriched Armillariella tabescens Polysaccharide and its preparation and use

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Experimental program
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Embodiment Construction

[0056] (1), the preparation of culture medium

[0057] Take 500ml of distilled water, add each component in the table below, then dilute to 1000ml with distilled water, sterilize and set aside.

[0058]

[0059] (2), fermentation culture and polysaccharide extraction

[0060] Under sterile conditions, the inoculum amount is 5g / L, inoculate the strain of Leucobacterium in the fermentation bottle with any medium in the above table, and carry out liquid static fermentation at 26°C for 20 days. After the cultivation, the bacterial strands of Leucobacterium zincrichum were collected, and the inorganic zinc in the bacterial strands was removed by continuous dialysis. The specific method is to repeatedly dialyze the strain of Leucobacterium zincum in double-distilled water containing 0.02% sodium azide until no amino acid is found in the dialysate by ninhydrin method. Then homogenate the zinc-rich bacteria cord after dialysis, extract it with 80°C water for 3 times, separate and...

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Abstract

The invention discloses a zinc-rich Armilariella tabescens polysaccharide, which is characterized by the following: fermenting in the zinc liquid culture medium to obtain polysaccharide as amorphous grey powder; improving extracting rate to 14% with 75-80% polysaccharide and 105-135mg / g organic zinc; restoring hematopoietic system and immune system function; avoiding radiation; fitting for making radiation-proof drug or hygienic food.

Description

1. Technical field [0001] The present invention relates to a fungus polysaccharide with both medicinal and edible uses and its preparation method and application, in particular to a fungus polysaccharide rich in micronutrient elements and its preparation method and application, to be precise, zinc-rich bright fungus polysaccharide and its preparation Methods and uses. 2. Background technology [0002] Bright fungus [Armillariella tabescens (Scop.ex Fr.) Sing], also known as Armillaria tabescens, is a basidiomycete with medicinal and edible properties discovered for the first time in my country. Traditional Chinese medicine believes that the fungus is cold in nature and sweet in taste, and has health effects such as strengthening muscles and bones, improving eyesight, benefiting the lungs, and benefiting the stomach. The polysaccharide extracted from it has various pharmacological activities such as immune regulation, liver protection, tumor suppression, and whitening. [0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C08B37/00C12N1/14A61K31/715
Inventor 沈业寿郑媛
Owner ANHUI PROVINCE KANGMEILAI DABIESHAN BIOTECHCO
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