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An extracting method of carnosine

An extraction method and carnosine technology, applied in the field of carnosine extraction, can solve the problems of jeopardizing production efficiency and high production cost, and achieve the effects of reducing production links, reducing production cost and simple preparation process

Inactive Publication Date: 2009-03-04
HAINAN STAND BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of harm, low production efficiency and high production cost in the existing carnosine preparation method, the purpose of the present invention is to provide a production process for extracting carnosine from animal skeletal muscle

Method used

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Examples

Experimental program
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Embodiment

[0030] Take 100.0 kg of fresh or frozen healthy beef that has passed the inspection, remove connective tissue, grease, bone fragments and other non-muscle tissue, wash with water for injection, drain, and weigh. First grind it into pieces with a meat grinder, grind it finely with a colloid mill with a scale adjusted to 200 mesh, collect the homogenate, add 150.0kg (1.5 times the weight) of water for injection and stir well. Warm the slurry to 42°C-45°C, adjust the pH to 1.5-1.7 with 4.5% hydrochloric acid, add 100,000 international units (specific activity) of pepsin per kg of slurry, and add 25 million international units (specific activity) of gastric Protease, after stirring well, maintain the conditions of pH 1.5-1.7, 42°C-45°C for 8 hours for hydrolysis. Use 18% NaOH to adjust the pH of the pepsin hydrolysis slurry to 7.5-7.7, add 10 million international units (specific activity) trypsin according to the ratio of 40,000 international units (specific activity) trypsin per...

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PUM

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Abstract

The invention discloses an extracting method of carnosine, which comprises the following steps: removing non-muscle tissue in the animal meat; washing; drying; grinding; adding water to stir into slurry; proceeding double-enzyme hydrolysis; proceeding enzyme inactivation; separating supernatant; adsorbing through hydroxy agustite chromatographic column; hyperfiltering through hyperfiltering film; collecting filtrate; spraying to dry carnosine liquid; collecting dried powder; packing in the vacuum.

Description

technical field [0001] The present invention relates to the production process of medicine and chemical industry, in particular to the method for extracting carnosine from animal skeletal muscle. Background technique [0002] Carnosine is a natural dipeptide found in muscle and consists of beta-alanine and histidine. Boldyrev (1988) etc. reported that carnosine has antioxidative effect and can inhibit the fat oxidation reaction caused by iron, copper, myoglobin and lipoxygenase. Carnosine has a stronger antioxidant effect than BHA, BHT, TBHQ, PG and α-tocopherol. Decker et al. (1990) also found that carnosine can inhibit the oxidation reaction catalyzed by iron, heme protein, lipoxygenase and elemental oxygen in vivo. Carnosine can also inhibit the formation of rancid smell and the change of color. The rancid smell of the sample added with carnosine is significantly lower than that of the sample added with BHT, α-tocopherol and sodium tripolyphosphate after storage for 1 m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02C07K1/36
Inventor 伍曾利
Owner HAINAN STAND BIO TECH
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