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Active largehead atractylodes saccharide complex for reducing blood sugar and its prepn and use

A complex and hypoglycemic technology, applied in the direction of organic active ingredients, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of unreported physical and chemical constants and structures, and achieve simple extraction and separation methods, high yield, Blood sugar lowering effect

Inactive Publication Date: 2008-12-03
SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mao Junhao et al. (Journal of Immunology, 1996, 4: 233) reported that the polysaccharide component PAM of Atractylodes macrocephala regulates the immune function of mouse spleen lymphocytes, and the physical and chemical constants and structures of this component have not been reported.

Method used

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  • Active largehead atractylodes saccharide complex for reducing blood sugar and its prepn and use
  • Active largehead atractylodes saccharide complex for reducing blood sugar and its prepn and use
  • Active largehead atractylodes saccharide complex for reducing blood sugar and its prepn and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] After crushing the rhizome of Atractylodes macrocephala, weigh 200g, add 2000ml of deionized water, soak at room temperature for 48 hours, and stir several times to facilitate the leaching of active ingredients. The soaking solution is first filtered with four layers of gauze, and then the filtrate is centrifuged at a speed of 4500r / min for 8 minutes. The supernatant was concentrated under reduced pressure, the temperature control range was 50°C-55°C, and the concentrated volume was up to 600ml. In the 600ml concentrated solution, add 3600ml of 95% ethanol to carry out alcohol precipitation, and the alcohol precipitation time is 24 hours. After the alcohol precipitation, the supernatant was poured out gently, and the precipitated part was centrifuged at a speed of 4500r / min for 8 minutes. Take out the precipitate and add 500ml of deionized water to dissolve it, and then centrifuge to remove the insoluble matter after dissolution. The supernatant was separated by dialy...

Embodiment 2

[0025] Wash and dry 1 kg of crude drug Atractylodes macrocephala, crush, soak in 10 liters of deionized water at room temperature for 24 hours, and filter. Continue soaking the residue with 5 liters of deionized water for 24 hours, filter, combine the filtrates, and concentrate under reduced pressure at 60°C to 2-4 liters. Then use 95% ethanol for precipitation, the final concentration of ethanol is 80%, and alcohol precipitation for 24 hours. Centrifuge, dissolve the precipitate with 2.5 liters of water, centrifuge, and dialyze with deionized water for 48-72 hours. Concentrate under reduced pressure at 60°C and then freeze-dry to obtain 12 grams of Atractylodes macrophage complex. Elemental analysis: C=37.99%, H=6.20%, N=2.19% Sugar content: 52.7%, uronic acid content: 15.9%; protein content: 16.7%.

Embodiment 3

[0027] Wash and dry 500 g of the crude drug Atractylodes macrocephala, pulverize, soak in 5 liters of deionized water at room temperature for 24 hours, and filter. The residue was soaked in 3 liters of deionized water for 24 hours, filtered, and the combined filtrates were concentrated to 2 liters under reduced pressure at 60°C. Then use 95% ethanol for precipitation, the final concentration of ethanol is 85%, and alcohol precipitation for 24 hours. Centrifuge, dissolve the precipitate with 1 liter of water, centrifuge, and dialyze with deionized water for 48-72 hours. Concentrate under reduced pressure at 60°C and then freeze-dry to obtain 6.2 g of Atractylodes saccharide complex. Elemental analysis: C=45.02%, H=6.01%, N=1.05%: sugar content: 58.0%; uronic acid content: 28.1%; protein content: 11.0%.

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Abstract

An active largehead atractylodes saccharide complex is produced through water soaking largehead atractylodes rhizome, organic solvent precipitating, water dissolving the precipitate, dialyzing or membrane separating the supernatant liquid, concentration and drying to obtain coarse product, column chromatographic separation of the coarse product to obtain fine largehead atractylodes saccharide compound. The process is simple and high in yield and is suitable for industrial production. The largehead atractylodes saccharide complex contains polysaccharide in 40-60 wt%, alduronic acid in 15-45 wt% and protein in 10-25 wt% and the polysaccharide includes glucose, galactose, mannose, arabinose and rhamnose. The largehead atractylodes saccharide complex has obvious blood sugar reducing effect and no toxic side effect.

Description

technical field [0001] The present invention relates to a sugar complex extracted from Chinese medicinal materials, a production method and application, in particular to an Atractylodes macrocephala sugar complex, a production method and an application. technical background [0002] Atractylodes macrocephala is a traditional Chinese medicinal material, which has the effects of "invigorating qi and invigorating the spleen, drying dampness and diuresis, antiperspirant, and anti-abortion. Atractylodes atractylodes contains volatile oils, such as atractylone, atractylodes lactone and trace elements. There are few reports on chemical and biological activity. Gu Yucheng et al. (Chinese herbal medicine, 1992, 10: 507) isolated two kinds of polysaccharides from Atractylodes Rhizoma Atractylodes Rhizome, wherein the molecular weight is 3.1×10 4 Mannan and molecular weight 1.1 x 10 4 fructan, no biological activity reported. Chi Yumei et al. (Chinese Medicinal Materials, 2001, 9:647...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61K36/284A61P3/10A61K31/7004
Inventor 田庚元单俊杰
Owner SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
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