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Bone material preserving fluid and its prepn and use

A bone material and preservation solution technology, which is applied in the field of bone material preservation solution and its preparation, can solve the problems of bone graft material fracture, bone graft failure, and bone graft material contamination, and achieve good tissue compatibility, increase antibacterial performance, low cost effect

Inactive Publication Date: 2008-11-05
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of incompletely rewarmed frozen bones and incompletely hydrated freeze-dried bones may cause fracture of bone graft materials; at the same time, cumbersome operations are likely to cause contamination of bone graft materials, resulting in failure of bone grafts

Method used

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  • Bone material preserving fluid and its prepn and use
  • Bone material preserving fluid and its prepn and use
  • Bone material preserving fluid and its prepn and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Preparation of bone material preservation solution of the present invention

[0050] Table 1 Preparation formula of bone material preservation solution

[0051]

[0052] According to the formula in Table 1, the bone material preservation solutions A, B, C, D, and E of the present invention are configured.

[0053] The configuration steps are as follows:

[0054] 1. Respectively take the medium that can promote the growth and proliferation of osteoblasts and dissolve them in pure water;

[0055] 2. Respectively dissolve the formulated amount of Tobramycin;

[0056] 3. Dissolve the formula amount of vitamin C in it;

[0057] 4. Dissolve dexamethasone in the formula respectively;

[0058] 5. Take the formula amount of sodium β-glycerophosphate and dissolve in it;

[0059] 6. After filter sterilization with Millipall decompression filter with membrane pore size of 0.22um, sealed and stored for later use.

Embodiment 2

[0060] Example 2 Preparation of the bone material of the present invention

[0061] Preparation of bone material: Take fresh allogeneic bone or xenogeneic bone (such as bovine bone, pig bone, etc.), remove attached soft tissue, cartilage, bone marrow, rinse with distilled water, and treat with 10% hydrogen peroxide at room temperature for 48-72 hours , Change the liquid once every 24 hours, rinse with distilled water, treat with ethanol for 8 hours at room temperature, rinse with distilled water, and process it into 6×0.5×0.5cm bone material according to the required specifications, -40℃10 -5 Pa freeze-drying for 24 hours, 25KGy γ-ray irradiation sterilization, every two pieces of materials are sealed with a double-layer medical plastic bag into a package, and the bone prepared in Example 1 of the present invention is added to the medical plastic bag in a sterile environment Material storage solutions A, B, C, D, E until the material is completely immersed, heat-sealed, and stored...

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Abstract

The present invention relates to one kind of bone material preserving fluid and its preparation and use. The bone material preserving fluid consists of osteoblast culture medium in 10-15g / L, sodium beta-glycerophosphate in 8-12 mmol / L, dexamethasone in 0.02- 0.08 mmol / L, vitamin C in 10-100 mg / L, tobramycin in 10<4>-10<6> U / L or tetracycline in 4-12 mg / L, and water as the solvent. The bone material preserving fluid is used in preserving bone repairing material, especially biologically derived bone material, at normal temperature. It can maintain the physical and chemical performances of bone material, enhance its antibiotic performance and cell compatibility, and raise its atopic osteoplastic effect and bone defect repairing effect.

Description

Technical field [0001] The invention belongs to the field of biological materials, and specifically relates to a bone material preservation liquid and a preparation method and application thereof. Background technique [0002] For bone defects caused by trauma, infection, tumors or congenital diseases, the use of allogeneic bone or xenograft to repair the bone defect is a common clinical method. There are currently two main types of preservation methods reported for bone materials: one is wet preservation, that is, preservation is soaked in a preservation solution; the other is cryopreservation, and the third is preservation at room temperature after freeze-drying. [0003] The preservatives used for wet preservation that have been reported include honey, whole blood or plasma, mercury sulfate, alcohol, formalin, chlorhexidine glycerin, dimethyl sulfoxide, etc. Some of them require cryogenic preservation It is cumbersome to use together and cannot overcome the shortcomings of cry...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/00
Inventor 罗静聪杨志明
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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