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The E type DNA vaccine of Chlamydi trachomatis and its preparation methods and applications

A technology of Chlamydia trachomatis and DNA vaccine, applied in the field of biomedicine, can solve the problem of weak protection against C.tE infection, and achieve the effects of strong repeatability, obvious effect and safe vaccination

Inactive Publication Date: 2008-06-04
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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Problems solved by technology

However, many data show that E type is the most common type of C.t in genitourinary tract infection, that is, E type is the dominant type of infection. Since the protective effect of C.t infection is "type-specific", the DNA vaccine of C.t D type has no effect on C.t E The protective effect of type infection is not strong

Method used

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  • The E type DNA vaccine of Chlamydi trachomatis and its preparation methods and applications
  • The E type DNA vaccine of Chlamydi trachomatis and its preparation methods and applications
  • The E type DNA vaccine of Chlamydi trachomatis and its preparation methods and applications

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Embodiment Construction

[0017] The present invention clones the omp1 gene encoding the main outer membrane protein from the chromosomal gene of Chlamydia trachomatis E-type BOUR strain, and clones it directionally into the eukaryotic expression vector pcDNA3.1(+) to construct the exogenous Chlamydia trachomatis inserted by DNA vaccine The structure of the E-type omp1 gene is described as follows:

[0018] (1) Sequence features

[0019] Length: 1182bp

[0020] Type: nucleic acid

[0021] Number of chains: double chain

[0022] Geometry: Linear

[0023] (2) Molecular type: DNA

[0024] (3) Chlamydia trachomatis E type BOUR strain (Tracoma type E strain BOUR), buy

[0025] From the American Type Culture Collection (ATCC), item number: VR-348BTM.

[0026] (4) The nucleotide sequence of the omp1 gene is described as follows:

[0027] atgaaaaaac tcttgaaatc ggtattagta tttgccgctt tgagttctgc ttcctccttg 60

[0028] caagctctgc ctgtggggaa tcctgctgaa ccaagcctta tgatcgacgg aattctgtgg 120

[0029] gaaggttt...

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Abstract

The invention provides preparation method and use of E-DNA vaccines of chlamydia trachoma. By means of molecular cloning and other genetic engineering technology, the invention clones the major outer membrane protein gene (CR genes) from E-chromosome gene of Chlamydia trachoma using PCR technology then directional connected with eukaryotic expression plasmid pcDNA3.1 (+) to construct the DNA vaccine. Confirmed through animal experiments, the constructed E-DNA vaccine of chlamydia trachoma induces mice to produce certain cell and humoral immunity specific to chlamydia trachoma. Cellular immunity is even more markedly, predominated with the Th1 reaction, and acts as a protection to the attacks of chlamydia trachoma to E-genital tract. The vaccine is expected to improve the high incidence of chlamydia trachoma infections in genitourinary tract, difficult to cure, easy to relapse and costly conditions.

Description

【Technical field】 [0001] The invention relates to the technical field of biomedicine, in particular to a Chlamydia trachomatis E-type DNA vaccine and its preparation method and application. 【Background technique】 [0002] Chlamydia trachomatis (C.t) has caused the widespread prevalence of trachoma worldwide, causing serious harm, and is still the leading cause of blindness in some developing countries. In the mid-1980s, it became the main pathogenic bacteria of sexually transmitted infections again, and the incidence rate ranked first in sexually transmitted infections. The incidence of sexually transmitted infections caused by Chlamydia trachomatis is rising rapidly in recent years. What is more serious is that C.t infection often has mild symptoms or even no symptoms, which cannot attract enough attention from people, cannot be diagnosed and treated in time, and persists for several months or even years, eventually causing pelvic inflammatory disease, infertility, ectopic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61P31/00A61P13/00A61P15/00
Inventor 刘全忠齐蔓莉王敬陈锦英
Owner GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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