Micro-molecular polypeptides capable of combining with gold staphylococcus virulence factor regulatory protein and its pharmaceutical use

A technology of virulence factor and protein regulation, which can be applied to peptide/protein components, medical preparations containing active ingredients, antibacterial drugs, etc.

Inactive Publication Date: 2008-01-23
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the clinical treatment of Staphylococcus aureus mostly adopts the combined use of antibiotics, but the effect is not ideal
Because Staphylococcus aureus is very prone to drug resistance and there is no good solution, many commonly used antibiotics are ineffective against it. Controlling Staphylococcus aureus infection is one of the problems to be solved in clinical medicine

Method used

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  • Micro-molecular polypeptides capable of combining with gold staphylococcus virulence factor regulatory protein and its pharmaceutical use
  • Micro-molecular polypeptides capable of combining with gold staphylococcus virulence factor regulatory protein and its pharmaceutical use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1. T Genetic Engineering Expression and Isolation and Purification of RAP Protein

[0020] The correctly identified Escherichia coli TRAP gene was connected to the expression vector, and expressed in Escherichia coli. After SDS-PAGE electrophoresis, it was found that there were bands of the target protein in the supernatant and inclusion bodies, and the target protein in the supernatant accounted for the total More than 50% of the amount. The target protein with His tag was purified by metal chelate column, detected by SDS-PAGE, the molecular weight was about 23kD, and the purity was >90%. The His tag was cut off with thrombin, and the molecular weight of the purified TRAP protein was about 21kD, and the purity was >95%. (see accompanying drawing 1, wherein 1: molecular weight protein standard; 2: the TRAP protein of purification.).

Embodiment 2

[0021] Example 2. Identification of Purified TRAP Proteins

[0022] Through WESTEN BLOT detection, our purified TRAP protein can specifically react with the TRAP protein polyclonal antibody (Figure 2)

Embodiment 3

[0023] Example 3. Screening of TRAP-binding peptides

[0024] First, 100 μl of purified TRAP protein was used to coat the enzyme-linked plate, and placed at 4°C overnight. After blocking with 2% gelatin for 1 h, add the phage dodecapeptide library, incubate at room temperature for 1 h, wash the non-specifically bound phage with TBST (50 mmol / LTris-HCl, 0.1% TWEEN20, pH 7.5), and wash with 0.2 mmol / L Glycine-HCl pH2.2 elutes the specifically bound phage, and the eluate is neutralized with 1mmol / L Tris-HCl pH9.0. According to the method provided by the kit, the titer of the phage in the eluate was determined, and the titer of the eluted phage of the uncoated target protein was used as a control to determine the input-output ratio. At the same time, the eluted phages combined with TRAP were amplified and their titers were determined for the next round of screening. After 3 rounds of screening, the input and output of the assay has been significantly improved. The enriched ph...

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Abstract

The invention relates to a group of micro-molecular polypeptides capable of combining with gold staphylococcus virulence factor regulatory protein (TRAP), these small molecular peptides have the amino acid sequence represented by the sequence table. The polypeptides can be applied in preparing staphylococcus aureus resistant medicament.

Description

technical field [0001] The present invention relates to a group of small molecular peptides capable of binding to the Staphylococcus aureus virulence factor regulatory protein TRAP (Target of RNAIII activating protein), the binding peptide can specifically inhibit the toxin production of Staphylococcus aureus, and the present invention also relates to the use of these small molecular peptides in Applications in the field of medicine. Background technique [0002] Staphylococcus aureus (Staphylococcus aureus) is a common Gram-positive pathogen, and it is one of the main microorganisms that cause fatal diseases such as burns and war wound infections, pneumonia, endocarditis, sepsis, and toxic shock. one. The number of people infected with Staphylococcus aureus in hospitals alone exceeds millions every year. At present, the combined use of antibiotics is often used in clinical treatment of Staphylococcus aureus, but the effect is not ideal. Because Staphylococcus aureus is v...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/04A61K38/00
CPCC07K7/08A61K38/00A61P31/04
Inventor 邵宁生杨光柳川高亚萍董洁丁红梅沈倍奋
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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