Production of fructosan by Cellulomonas sp.nov.GJT07 strain

A technology of fructan and application, which is applied in the field of fructan production with Cellulomonas sp.nov.GJT07 strain, and can solve the problem that there is no cellulomonas and the like

Inactive Publication Date: 2007-09-05
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the production of fructan by Cellulomonas microorganisms at home and abroad.

Method used

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  • Production of fructosan by Cellulomonas sp.nov.GJT07 strain
  • Production of fructosan by Cellulomonas sp.nov.GJT07 strain

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Experimental program
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Embodiment Construction

[0010] Obtaining of the Cellulomonas sp.nov.GJT07 strain of the present invention: collect soil samples from the orchard, add them to a conical flask containing 25ml of enrichment medium, and culture in a shaker flask at 30°C and 160r / min for 48h. Then, after diluting the enriched culture solution 10 times with sterile physiological saline, take 0.2 ml to coat the separation medium plate. Inverted culture at 30°C for 48 hours, colonies with viscous and transparent appearance on the plate were obtained, and a bacterium capable of producing exopolysaccharide was obtained. According to the morphological observation and physiological and biochemical identification of the strain, it was determined to be Cellulomonas according to the ninth edition of Bergey's Manual. Enrichment medium consists of 2g sucrose, 3g yeast extract, NaNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 0.5g, NH 4 Cl 0.5g, FeSO 4 0.01g composition, add distilled water 1000ml, adjust pH 7.5; separation medium consists...

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Abstract

The invention is about the Cellulomonas sp.nov.GJT07 which can secrete the fructosan. The strain which is separated from the soil of the orchard is same as the Cellulomonas with the morphological and the physiological-biochemical characteristic according to the ninth Bergs handbook. The strain has the good stability, so it can reach the industrial production.

Description

field of invention [0001] The invention relates to a microorganism Cellulomonas sp.nov. GJT07 capable of producing fructan and a method for producing fructan by using the strain. Background of the invention [0002] Fructan is the most widely distributed biopolymer in nature, and it is a homopolysaccharide composed of fructofuranose groups linked by β-2,6-bonds and β-2,1-bonds. Fructans are produced in plants as carbohydrate storage substances. As far as the inventors know through consulting information and literature search, some microorganisms can also produce fructan, including bacteria, yeast and fungi. Such as: Acetobacter, Actinomyces, Aspergillus, Bacillus, Corynebacterium, Erwinia, Pseudomonas and Streptococcus. However, there is no report on the production of fructan by Cellulomonas microorganisms at home and abroad. [0003] The qualitative and quantitative analysis methods of fructan mainly adopt: spectrophotometry and chromatography. Among them, chromatograph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/04
Inventor 江晓路张红霞
Owner OCEAN UNIV OF CHINA
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