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Compositon for preventing or treating osteoporosis

a technology for osteoporosis and composition, applied in the field of composition for preventing or treating osteoporosis, can solve the problems of difficult control of when to administer, reported side effects and disadvantages, and achieve the effects of increasing bioaffinity, inhibiting bone resorption, and increasing bone formation

Inactive Publication Date: 2021-01-07
PENS CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The composition described in this patent can help increase bone formation and decrease bone resorption, which can prevent or treat osteoporosis. It is made from natural products, such as a Gentiana scabra extract and a Sambucus williamsii extract, which are safe and effective. This formula can provide a stable and reliable way to prevent or treat osteoporosis.

Problems solved by technology

As a known treatment method of osteoporosis, there are estrogen therapy, calcitonin therapy, calcium supplements, vitamin D derivative therapy, and so on, and estrogen therapy and calcitonin therapy have been reported as the most commonly used treatment methods, but they have been reported to have various side effects and disadvantages that are difficult to control when to administer.
However, the above-mentioned prior arts shows that the proliferation and differentiation ability of osteoblasts or the expression of factors related to osteoblasts may be increased by using natural products, but since they do not disclose the effect on bone resorption by osteoclasts, it is necessary to develop a therapeutic agent for osteoporosis of a new natural material capable of maintaining homeostasis of bone formation and bone resorption.

Method used

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  • Compositon for preventing or treating osteoporosis
  • Compositon for preventing or treating osteoporosis
  • Compositon for preventing or treating osteoporosis

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

Preparation of Gentiana scabra and Sambucus williamsii Extracts

[0090]In order to prepare a Gentiana scabra extract and a Sambucus williamsii extract, 2 L of distilled water was placed into each 100 g of the original and hot water extraction was carried out at 90° C. for 5 hours. The filtered extract solutions were concentrated under reduced pressure at 50° C. to obtain samples in powder form through lyophilization. The yields of the Gentiana scabra extract and the Sambucus williamsii extract were 15.4% and 9.27%, respectively.

experimental example 1

Cytotoxicity Evaluation

[0092]In this experimental example, an experiment was carried out to confirm the effect on the cytotoxicity when treated with the Sambucus williamsii extract and the Gentiana scabra extract of the present invention.

[0093]1×104 MC3T3-L1 cells were seeded into a 96-well plate, and DMEM containing 10% FBS was used as the culture medium. After incubation for 12 hours, each extract (Examples 1 to 5) was treated at 100-1000 μg / ml and incubated for 24 hours. Then, 20 μl of WST reagent was added thereto, light was blocked with foil to minimize WST reduction by light, and the reaction was carried out for 3 hours. Optical density was measured at a wavelength of 560 nm using an iMARK™ Microplate Reader (Bio-Rad Laboratories Headquarters, Hercules, Calif., USA).

[0094]The obtained results of the cytotoxicity for each extract with ED50 (which is a concentration at which cell viability is 50%) are shown in Table 2 below. As a result, it was shown that the Gentiana scabra ext...

experimental example 2

Alkaline Phosphatase Activity (ALP Activity) Measurement

[0095]In this experimental example, an experiment was carried out to confirm the effect on the alkaline phosphatase acitivity when treated with the Gentiana scabra extract and the Sambucus williamsii extract of the present invention by concentration. On the other hand, alkaline phosphatase (ALP; basic phosphatase) is present in most tissues, and ALP present in bone tissue is known to increase its activity when bone growth is actively occurring.

[0096]5×103 MC3T3-L1 cells were seeded into a 6-well plate, and 50 μg / ml ascorbic acid and 10 mM β-glycerophosphate were added to induce differentiation into osteoblasts. After they were treated with each of Reference Examples 1 and 2 and Examples 1 to 3 at concentrations of 50 μg / ml, 100 μg / ml, and 200 μg / ml, and incubated for 48 hours at 37° C. in 5% CO2 incubator, and then washed twice with PBS, and 0.2% triton X-100 was added thereto, and subsequently, lysis was carried out in a 37° C...

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Abstract

The present invention relates to a composition for preventing or treating osteoporosis, comprising a Gentiana scabra extract and a Sambucus williamsii extract, and it is an object of the present invention to provide the composition for preventing or treating osteoporosis, which is capable of increasing bone formation and inhibiting bone resorption. In addition, it is another object of the present invention to provide the composition for preventing or treating osteoporosis, which increases bioaffinity and has no side effects by comprising a Gentiana scabra extract and a Sambucus williamsii extract, which are natural products, thereby stably exhibiting an effect of preventing or treating osteoporosis.

Description

TECHNICAL FIELD[0001]This application claims the benefit of priority based on Korean Patent Application No. 10-2019-0049946, filed on Apr. 29, 2019, and the entire contents disclosed in the literatures of said Korean Patent Application are incorporated as part of the present specification.[0002]The present invention relates to a composition for preventing or treating osteoporosis, comprising a Gentiana scabra extract and a Sambucus williamsii extract.BACKGROUND ART[0003]Bone metabolism disease is emerging as a major problem as we enter the aging society, and osteoporosis accounts for the largest proportion of bone metabolic diseases. On the other hand, osteoporosis is a disease in which the composition of the skeleton is changed due to the imbalance of calcium metabolism, thereby reducing bone mass and easily causing fractures of the radius, femoral, and vertebrae, and in particular has the highest incidence in post-menopausal women, and estrogen secretion has been reported as the m...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K36/515A61K36/35A61P19/10
CPCA61K36/515A61K36/35A61K2236/37A61K2236/331A61P19/10A61K36/48A61K2300/00
Inventor KIM, HONG SIK
Owner PENS CO LTD
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