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Compositions for treating spinal muscular atrophy

a technology of spinal muscular atrophy and compositions, applied in the direction of muscular disorder, drug compositions, dispersed delivery, etc., can solve the problems of muscle weakness, poor muscle tone, weak cry,

Inactive Publication Date: 2018-10-11
F HOFFMANN LA ROCHE & CO AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent is about a new treatment for spinal muscle atrophy (SMA), which has several benefits. It can reduce the severity of SMA, delay the onset of symptoms, inhibit the progression of symptoms, reduce hospitalization, improve quality of life, alleviate symptoms, reduce duration of symptoms, prevent symptoms from recurring, inhibit the development of new symptoms, and prevent the progression of new symptoms. The treatment involves a specific compound, and it can be formulated with a sweetener and flavor to make it easier to drink.

Problems solved by technology

Symptoms include muscle weakness, poor muscle tone, weak cry, limpness or a tendency to flop, difficulty sucking or swallowing, accumulation of secretions in the lungs or throat, feeding difficulties, and increased susceptibility to respiratory tract infections.
The legs tend to be weaker than the arms and developmental milestones, such as lifting the head or sitting up, cannot be reached.
The severe forms of the disease are fatal and all forms have no known cure.
Infants with a severe form of SMA frequently succumb to respiratory disease due to weakness in the muscles that support breathing.
Nevertheless, SMN2 is unable to compensate completely for the loss of SMN1 function due to alternative splicing of exon 7 caused by a translationally silent C to T mutation in exon 7.
Medical care for SMA patients at present is limited to supportive therapy including respiratory, nutritional and rehabilitation care; there is no drug known to address the underlying cause of the disease.
The major management issue in Type 1 SMA is the prevention and early treatment of pulmonary problems, which are the cause of death in the majority of the cases.
As a result of improved understanding of the genetic basis and pathophysiology of SMA, several strategies for treatment have been explored, but none have yet demonstrated success in the clinic.
Aminoglycosides have been shown to enhance expression of a stabilized SMN protein produced from Δ7 SMN2 mRNA by promoting the translational read-through of the aberrant stop codon, but have poor central nervous system penetration and are toxic after repeat dosing.
Chemotherapeutic agents, such as aclarubicin, have been shown to increase SMN protein in cell culture; however, the toxicity profile of these drugs prohibits long-term use in SMA patients.
However, the use of the HDAC inhibitors or mRNA stabilizers does not address the underlying cause of SMA and may result in a global increase in transcription and gene expression with potential safety problems in humans.

Method used

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  • Compositions for treating spinal muscular atrophy
  • Compositions for treating spinal muscular atrophy
  • Compositions for treating spinal muscular atrophy

Examples

Experimental program
Comparison scheme
Effect test

example 1

2-(2-methylimidazo[1,2-b]pyridazin-6-yl)-7-(4-methylpiperazin-1-yl)pyrido[1,2-a]pyrimidin-4-one

[0550]

[0551]In a sealed tube, 7-fluoro-2-(2-methylimidazo[1,2-b]pyridazin-6-yl)-4H-pyrido[1,2-a]pyrimidin-4-one (Intermediate 1; 35 mg, 0.119 mmol) and 1-methylpiperazine (47.5 mg, 0.474 mmol, 4 eq.) were stirred in DMSO (1 mL) at 120° C. overnight. LC-MS showed total convertion. The solvent was removed under high vacuum. The crude product was purified by column chromatography (SiO2, CH2Cl2 / MeOH=95 / 5 to 9 / 1) to afford the title product (25 mg, 56%) as a light yellow solid. MS m / z 376.3 [M+H+].

example 2

7-[(8aR)-3,4,6,7,8,8a-hexahydro-1H-pyrrolo[1,2-a]pyrazin-2-yl]-2-(2-methylimidazo[1,2-b]pyridazin-6-yl)pyrido[1,2-a]pyrimidin-4-one

[0552]

[0553]In a sealed tube, 7-fluoro-2-(2-methylimidazo[1,2-b]pyridazin-6-yl)-4H-pyrido[1,2-a]pyrimidin-4-one (Intermediate 1; 125 mg, 0.426 mmol) and (R)-octahydropyrrolo-[1,2-a]pyrazine (160 mg, 1.27 mmol, 3 eq.) were stirred in DMSO (5 mL) at 125° C. overnight. The solvent was removed under high vacuum. The residue was taken up in CH2Cl2 and washed with an aqueous saturated solution of NaHCO3. The organic layer was separated and dried over Na2SO4 and concentrated in vacuo. The crude was purified by column chromatography (SiO2, CH2Cl2 / MeOH=98 / 2 to 95 / 5) to afford the title product (65 mg, 38%) as a light yellow solid. MS m / z 402.5 [M+H+].

example 3

7-[(8aS)-3,4,6,7,8,8a-hexahydro-1H-pyrrolo[1,2-a]pyrazin-2-yl]-2-(2,8-dimethylimidazo[1,2-b]pyridazin-6-yl)pyrido[1,2-a]pyrimidin-4-one

[0554]

[0555]In a sealed tube, 2-(2,8-dimethylimidazo[1,2-b]pyridazin-6-yl)-7-fluoro-pyrido[1,2-a]pyrimidin-4-one (Intermediate 2; 200 mg, 0.647 mmol) and (S)-octahydropyrrolo-[1,2-a]pyrazine (286 mg, 2.26 mmol, 3.5 eq.) were stirred in DMSO (5 mL) at 125° C. overnight. The solvent was removed under high vacuum. The residue was taken up in CH2Cl2 and washed with an aqueous saturated solution of NaHCO3. The organic layer was separated and dried over Na2SO4 and concentrated in vacuo. The crude was purified by column chromatography (SiO2, CH2Cl2 / MeOH=98 / 2 to 95 / 5) to afford the title product (115 mg, 43%) as a light yellow solid. MS m / z 416.3 [M+H+].

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Abstract

The present invention provides pharmaceutical compositions comprising a compound of formula (I)wherein A, R1, R2 and R3 are as described herein, as well as pharmaceutically acceptable salts thereof. Further the present invention is concerned with the manufacture of the pharmaceutical compositions comprising a compound of formula (I) and their use as medicaments.

Description

INTRODUCTION[0001]The present invention provides pharmaceutical compositions comprising compounds which are SMN2 gene splicing modulators, their manufacture and their use for the treatment, delay of progression or amelioration of spinal muscular atrophy (SMA). Further, the pharmaceutical compositions of the invention may optionally comprise cytoprotectors. The invention further relates to the combined use of SMN2 gene splicing modulators and cytoprotectors for use in the treatment or amelioration of spinal muscular atrophy (SMA).[0002]In particular, the present invention relates to pharmaceutical compositions comprising compounds of formula (I)[0003]wherein A, R1, R2 and R3 are as described herein, and pharmaceutically acceptable salts thereof.BACKGROUND[0004]Spinal muscular atrophy (SMA), in its broadest sense, describes a collection of inherited and acquired central nervous system (CNS) diseases characterized by progressive motor neuron loss in the spinal cord and brainstem causin...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K31/519A61K9/08A61K9/00A61K9/14A61K47/12A61K47/26A61K47/22A61K47/18
CPCA61K31/519A61K9/08A61K9/0053A61K9/145A61K47/12A61K47/26A61K47/22A61K47/183A61K2300/00A61K31/575A61K9/0095A61K9/14A61K31/5025A61P21/00A61P25/00A61P25/02A61P25/28A61K45/06
Inventor ALSENZ, JOCHEMGRASSMANN, OLAFKUEHL, PETERMETZGER, FRIEDRICHMCCARTHY, KATHLEEN DOROTHYVIANNA, EDUARDO PAULO MORAWSKIWOODHOUSE, MARVIN LLOYD
Owner F HOFFMANN LA ROCHE & CO AG
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