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Portable nucleic acid analysis system and high-performance microfluidic electroactive polymer actuators

a nucleic acid analysis and microfluidic electroactive technology, applied in biochemistry apparatus and processes, laboratories, instruments, etc., can solve the problems of nucleic acid diagnostics that cannot be processed in commercial instruments, cannot be configured to accept raw biological samples, and cannot develop a multiplex, automated, integrated “sample to result” system, etc., to achieve the effect of minimizing cross-contamination and maintaining sterility

Pending Publication Date: 2017-03-02
SRI INTERNATIONAL
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  • Abstract
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  • Application Information

AI Technical Summary

Benefits of technology

[0027]detection is based on probe sets rather than primer sets (easier to build new tests).

Problems solved by technology

The integration of sample preparation with amplification and detection in an easy-to-use system remains a significant challenge for nucleic acid diagnostics.
The research literature is rife with examples of pathogen detection techniques and devices; however, no one has been able to develop a multiplex, automated, integrated “sample to result” system, configured to accept raw biological samples.
However, despite decades of investment, no commercial instruments are available that perform nucleic acid processing at the point of need.

Method used

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  • Portable nucleic acid analysis system and high-performance microfluidic electroactive polymer actuators
  • Portable nucleic acid analysis system and high-performance microfluidic electroactive polymer actuators
  • Portable nucleic acid analysis system and high-performance microfluidic electroactive polymer actuators

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THEREOF

[0090]Our invention provides a portable, inexpensive molecular diagnostic system capable of producing results with no human intervention—human intervention is only needed to input the sample and to read the results. The results are also produced very quickly due to the various techniques described above. The system includes a method to interface with a disposable member, called the iMFC. The methods and the systems allow the iMFC to be configured easily to run different types of tests without the need to modify the underlying hardware. Finally, the card itself is modular, readily modifiable for running different types of tests.

[0091]In an aspect the invention provides a self-contained system that can take a sample as the input, perform molecular diagnostic steps and display the result of the diagnostic tests. Typically, the steps include (i) extraction and purification, e.g. where the DNA sample is extracted from the input sample, which may be blood, tissue, urine, saliva or ...

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Abstract

Devices, systems and methods for the parallel detection of a set of distinct nucleic acid sequences use multiple sequence amplification and simultaneous hybridization readout. An automated nucleic acid analysis system comprises in microfluidic connection sample lysis, purification, PCR and detection modules configured to detect in parallel distinct nucleic acid sequences via multiple sequence amplification and simultaneous microarray hybridization readout. High performance microfluidic electroactive polymer (μEAP) actuators comprising a dead-end fluid chamber in which the floor of the chamber is an electrode covered with an EAP layer of dielectric elastomer are configured for particle sorting.

Description

[0001]This application claims priority to Ser. No. 62 / 081,525; filed Nov. 18, 2014, to 62 / 041,430; filed Aug. 25, 2014, and to Ser. No. 61 / 979,377; filed Apr. 14, 2014.[0002]This invention was made with government support under Defense Advanced Research Projects Agency under contract no. HR0011-14-C-0082. The government has certain rights in the invention.INTRODUCTION[0003]The integration of sample preparation with amplification and detection in an easy-to-use system remains a significant challenge for nucleic acid diagnostics. [1] A number of systems have been approved by the FDA as “moderate complexity” devices for use in near-POC applications [2], including Cepheid's GeneXpert platform, Nanosphere's Verigene platform, the BD Max System from Becton, Dickinson and Company, and Liat's “lab-in-a-tube” disposable device.[0004]The research literature is rife with examples of pathogen detection techniques and devices; however, no one has been able to develop a multiplex, automated, inte...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/68B01L3/00
CPCC12Q1/686B01L3/502715B01L3/502738B01L3/502723B01L3/502746B01L2200/028B01L2400/082B01L2300/0654B01L2200/0684B01L2300/087B01L2300/0819B01L2300/168B01L2300/0887B01L2200/10C40B60/12B01L7/52B01L2300/0816B01L2300/1822B01L2400/0487B01L2300/1805G01N27/4473G01N27/44791
Inventor BALOG, ROBERTSECHLER, NINA
Owner SRI INTERNATIONAL
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