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Microbial inoculants and fertilizer compositions comprising the same

Inactive Publication Date: 2014-08-21
TERRAGEN HLDG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent describes a method for improving plant growth and productivity by applying a microbial inoculant or fertilizer composition to plants or soil. The methods can lead to healthier plants, better soil quality, and increased yield of plants and products.

Problems solved by technology

Such chemical fertilizers can be expensive to produce, can be hazardous to use and are often associated with environmentally damaging consequences, such as nitrate contamination in run off and ground water.
However bacteria of these genera are often unable to compete effectively with native soil and plant flora, thereby requiring the application of impractically large volumes of inoculum.
To date, biofertilizers have typically met with limited success, often not proving to be efficacious under real farming conditions.

Method used

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  • Microbial inoculants and fertilizer compositions comprising the same
  • Microbial inoculants and fertilizer compositions comprising the same
  • Microbial inoculants and fertilizer compositions comprising the same

Examples

Experimental program
Comparison scheme
Effect test

example 1

Microbial Strains

[0065]The following microbial strains were used in the production of a biofertilizer.

[0066]Lactobacillus parafarraginis Lp18 was isolated from an environmental source. Partial 16S rRNA sequencing indicated 100% similarity to Lactobacillus parafarraginis AB 262735 which has a risk group of 1 (TRBA). When cultured on MRS media for 3 days at 34° C., anaerobically, Lp18 produces cream, round, slight sheen, convex, colony diameter 1-2 mm (facultative anaerobe). Its microscopic appearance is Gram positive, non-motile, short rods rectangular, mainly diploid. Lactobacillus parafarraginis Lp18 was deposited with the National Measurement Institute, Australia on 27 Oct. 2011 under Accession Number V11 / 022945.

[0067]Lactobacillus buchneri Lb23 was isolated from an environmental source. Partial 16S rRNA sequencing indicated 99% similarity to Lactobacillus buchneri AB 429368 which has a risk group of 1 (TRBA). When cultured on MRS media for 4 days at 34° C., anaerobically, Lb23 pr...

example 2

Pasture Trials

[0079]Field trials on pasture were conducted using a biofertlizer as disclosed herein, in comparison to untreated pasture and pasture treated with conventional inorganic fertilizers.

[0080]The biofertilizer (hereinafter “IMP Bio”) comprised the six microbial strains listed in Example 1, at final concentrations of 2.5×105 cfu / ml for each of the Lactobacillus strains, 1.0×105 cfu / ml for Candida ethanolica and 1.0×106 cfu / ml for Acetobacter fabarum. The strains were grown as described in Example 1 and mixed with 2% trace elements, 0.3% humate (Soluble Humate, Lawrie Co), 3% molasses and 0.1-0.2% phosphoric acid. Phosphoric acid was added to the point where pH was in the range 3.8 to 4.0. The trace elements component typically comprised the following (per 1,000 L):

TABLE 1Trace elements component of biofertilizerMaterialVolume (kg)Water200kgPotassium Sulphate15.25kgCopper Complex125.6kgMagnesium Citrate2175.0kgChromium Citrate310.0kgCalcium Sokolate452.0kgCitric Acid11.15kgF...

example 3

Soil Quality

[0087]To determine the effect of a biofertilizer as disclosed herein on soil quality, 2×150 g of soil from a farm in Tasmania were each weighed into 2× clean 150 ml Schott bottle. 10 mls of a 1:10 dilution of IMP Bio fertilizer (see Examples 1 and 2) was dripped over the top of the soil in one bottle and the lid replaced and incubated at 34° C. for one week. The second bottle had no biofertilizer added was incubated 34° C. The soil from both bottles was analysed by Environmental Analytical Laboratories (EAL, Southern Cross University Lismore, NSW) using standard soil testing methods.

[0088]The results for the one week treatment of soil with IMP Bio are summarised in Table 4. Soil tests on the untreated incubated sample are not shown as these were substantially the same as the initial untreated soil test. It is clear from the soil tests on the two treated samples that there is a marked difference in the soil after incubation with IMP Bio. The second sample analysed, shows ...

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Abstract

Provided herein are microbial inoculants for use in increasing plant growth, plant productivity and / or soil quality, comprising strains of one or more bacterial species selected from Lactobacillus parafarraginis, Lactobacillus buchneri, Lactobacillus rapi and Lactobacillus zeae. Optionally the microbial inoculants also comprise a strain of Acetobacter fabarum and / or a strain of Candida ethanolica. Also provided are fertilizer compositions comprising said microbial inoculants.

Description

FIELD OF THE ART[0001]The present disclosure relates generally to microbial inoculants, particularly for use as fertilizers, comprising one or more microbial species or strains as described herein, and to fertilizer compositions comprising such organisms. The disclosure also relates to methods of promoting plant growth, increasing availability of nutrients in the soil and remediating degraded soils and pastures using microbial inoculants and fertilizer compositions of the present disclosure.BACKGROUND[0002]The use of fertilizers to enhance plant and crop production and overcome poor soil quality is widespread. Most commonly employed commercially available fertilizers are inorganic chemical fertilizers. Such chemical fertilizers can be expensive to produce, can be hazardous to use and are often associated with environmentally damaging consequences, such as nitrate contamination in run off and ground water. Environmental sustainability can be promoted by limiting the use of chemical f...

Claims

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Application Information

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IPC IPC(8): C05F11/08C12R1/225
CPCC12R1/225C05F11/08C09K17/00C12N1/20C12N11/02C12N1/16C05F11/02Y02P20/145C05F17/939Y02W30/40C12R2001/02C12N1/205C12R2001/225C12R2001/72C12N1/165A01G2/00C12N1/00
Inventor FINLAYSON, WAYNEJURY, KAREN
Owner TERRAGEN HLDG
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