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Novel biosurfactant produced by aureobasidium pullulans

a biosurfactant and aureobasidium pullulans technology, applied in the direction of surface-active detergent compositions, organic chemistry, detergent compositions, etc., can solve the problems of water pollution, serious environmental problems, chemically synthesized surfactants are not easily degradable, etc., to confirm the activity of the surfactant and verify the novelty of the compound

Inactive Publication Date: 2014-02-27
GYEONGBUK INST FOR MARINE BIOIND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemically synthesized surfactants are manufactured via a complicated process, are threatening to aquatic ecosystems due to their formation of bubbles on water surfaces thereby blocking the penetration of sunlight and oxygen, and become a cause of water pollution due to eutrophication induced from phosphates that are formed from phosphorus added to the surfactants to improve detergency.
In addition, chemically synthesized surfactants are not easily degradable, due to very low biodegradability, and they readily accumulate in the ecosystem, thereby causing serious environmental problems.
Furthermore, biosurfactants can be used for highly specific purposes due to their complex chemical structures and they are not easily synthesized by conventional methods.
However, it has not yet been reported that Aureobasidium pullulans strains produce biosurfactants.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Isolation of L9 from Aureobasidium Pullulans and Purification of the Same

[0042]Aureobasidium pullulans L-3-GPY was deposited at the Korean Culture Center of Microorganisms (KCCM) on Jul. 5, 2011 under accession number KCCM 11200P. 100 g of a sample prepared by concentrating a broth filtrate of Aureobasidium pullulans L-3-GPY isolated from Lilium lanciforlium Thunb was dissolved in water, and flash reversed-phase column chromatography was performed. Elution was attempted using 70% and 90% aqueous methanol, and a compound having activity according to an embodiment of the present invention was eluted in 90% aqueous methanol. The eluted active ingredient was concentrated under reduced pressure to remove methanol and treated with ethyl acetate (EA) to perform phase separation. As a result of measuring activity, activity was detected in the ethyl acetate phase. Then, ethyl acetate was concentrated under reduced pressure, and silica gel chromatography was performed using a solution of chlo...

example 2

Interpretation of Chemical Structure of Active Ingredient L9 using Spectroscopy

[0043]1. Measurement and Interpretation of NMR Spectra

[0044]In order to confirm the chemical structure of active Compound L9, Compound L9 was dissolved in CD3OD, and 1H NMR, 13C NMR, DEPT, 1H-1H COSY, HMQC, HMBC, and NOESY spectra were measured and interpreted.

[0045]1) Measurement and interpretation of 1H NMR spectrum: As a result of measuring 1H NMR spectrum (FIG. 2), 11 protons derived from oxygenated methines (tertiary carbons) and methylenes (secondary carbons) were observed at 3.5 to 5.0 ppm. In addition, 6 protons derived from three methylenes connected to a carbonyl group were observed at 2.4 to 2.6 ppm. Further, a plurality of methylene protons derived from an alkyl group were observed at 1.2 to 1.8 ppm, and 9 protons derived from three methyl groups were observed at around 0.8 ppm.

[0046]2) Measurement and interpretation of 13C NMR spectrum: As a result of measuring 13C NMR spectrum (FIG. 3), thre...

example 3

Observation of Performance as Surfactant

[0053]1) Measurement of Surface Tension

[0054]Compound L9 isolated in Example 1 was dropped on a hydrophobic film, and surface tension variation was observed using a surface tension meter (Densitometer, Sigma 700 Densitometer (KSV Instruments Ltd., Finland)). Water was a control, and a culture medium and the size of compound L9 were compared. The results are shown in FIG. 14.

[0055]As intermolecular interaction increases, surface tension increases. Since hydrocarbons, organic polymers, and the like have week intermolecular interactions, the surface tension thereof is low.

[0056]The numerical value of the surface tension was shown as (dyne / cm (or mN / m)). When a surfactant that has a hydrophobic moiety and a hydrophilic moiety is added to water, surface tension decreases. Table 1 below shows surface tension of water, mercury, and glycerin. As the intermolecular interaction increases, the surface tension increases.

[0057]Compound L9 according to the ...

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Abstract

Disclosed is a novel compound having biosurfactant activity that is produced by Aureobasidium pullulans L-3-GPY strains (KCCM11200P).The novelty of the structure of the compound having biosurfactant activity was evaluated using spectroscopy, and it was determined that the biosurfactant produced by the strains has excellent surfactant activity.Active ingredients of the biosurfactant may be used for various purposes, for example, for producing a composition for washing and cleaning agents. Furthermore, they can be used in a variety of industrial fields where chemically synthesized surfactants are used such as medicines, foods, cosmetics, onshore and offshore oil decontamination, degradation of oil and fat in treatment tanks, and the like where chemically synthesized surfactants are used.

Description

CROSS-REFERENCE TO RELATED APPLICATION[0001]This application claims under 35 U.S.C. §119(a) the benefit of Korean Patent Application No. 10-2012-0093185 filed Aug. 24, 2012, the entire contents of which are incorporated herein by reference.BACKGROUND[0002](a) Technical Field[0003]The present invention relates to a novel biosurfactant produced by Aureobasidium pullulans. [0004](b) Background Art[0005]The present invention relates to a biosurfactant produced by Aureobasidium pullulans) sp. L-3-GPY that is a biosurfactant-producing strain.[0006]A surfactant is a substance having both a hydrophilic moiety and a hydrophobic moiety in a single molecule. This characteristic can change the nature of the surface of a material and / or the interface between materials, thereby reducing a material's surface tension. In an aqueous phase, a surfactant reduces the attractive forces between water molecules. As the concentration of the surfactant increases, a micelle structure can form. A micelle stru...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C07C69/675
CPCC07C69/675A61K8/375A61K2800/805A61Q19/10C11D1/667C12P7/625A61K8/37C07C69/34C12P7/62
Inventor KIM, JONG SHIKPARK, NYUN HOKIM, CHOONG GON
Owner GYEONGBUK INST FOR MARINE BIOIND
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