Identification of protein binding sites

Abstract

The disclosure relates to the field of molecular recognition or detection of discontinuous or conformational binding sites or epitopes corresponding to a binding molecule, in particular, in relation to protein-protein, protein-nucleic acid, nucleic acid-nucleic acid or biomolecule-ligand interactions. Provided is a synthetic molecular library allowing testing for, identification, characterization, or detection of a discontinuous binding site able to interact with a binding molecule, the library having been provided with a plurality of test entities, each test entity comprising at least one first segment spotted next to a second segment, each segment having the capacity of being a potential single part of a discontinuous binding site.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of co-pending application Ser. No. 10 / 411,869, filed on Apr. 10, 2003, now U.S. Pat. No. ______, which is a continuation of International Application PCT / NL01 / 00744, filed Oct. 10, 2001, designating the United States, published in English Apr. 18, 2002, as WO 02 / 31510 A1, which claims priority from EP 00203518.6 filed Oct. 11, 2000, the contents of each of which are incorporated herein by this reference.FIELD OF THE INVENTION[0002]The invention relates to the field of molecular recognition or detection of discontinuous or conformational binding sites or epitopes corresponding to or interacting with a binding molecule, in particular, in relation to protein-protein or protein-ligand interactions.BACKGROUND OF THE INVENTION[0003]Interactions between binding molecules, which in general are biomolecules and their corresponding ligands, are central to life. Cells often bear or contain receptor molecules that i...

AI Technical Summary

Benefits of technology

This approach enables rapid and straightforward identification of discontinuous binding sites, facilitating the development of compounds that can modulate specific interactions, and provides a diverse library of binding bodies that can interact with binding molecules, offering advantages over traditional methods by not requiring prior identification of site components.

Problems solved by technology

However, with existing libraries, no attention is given to synthesizing specific segments in close proximity to each other so that they together can represent a putative binding site.

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